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Neotinea maculata. A: dry seeds; B: germinated seeds in water agar (WA) at the developing rhizoids stage; C: germinated seed in modified (type 1) Malmgren (Mm) at the protocorm plus initial shoot stage. Scale bars = 1 mm.
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The asymbiotic seed germination of orchids is a complex process with considerable technical difficulties, due to the natural requirement of a plant-fungus symbiotic relationship under field conditions. Only on rare occasions, orchid seeds are known to readily germinate in water. Neotinea maculata is a relatively common orchid species with its main...
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... of the species to readily germinate in water agar without any nutrients added, while we additionally reveal the inhibition of seed germination by light for this species. Seeds sown in nutrient media reached a stage 4-5 in the Zettler & Hofer scale (Zettler & Hofer 1998), while seeds imbibed in water agar reached stage 1-2 in the same scale (Fig. 1). The optimal germination temperature was 20°C and the highest, reported so far, germination (96.1%) was achieved in Mm medium. High germination (84.0%) was also achieved in water agar with no seed pretreatment, a result that seems to indicate the water permeability of the seed testa in this species. N. maculata is one of the few orchid ...
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Citations
... currently, a total of 56 taxa, i.e. more than one third of the orchid flora of Greece are conserved in the NKUA Seed Bank. During the ongoing PhD research, both the germinability (Oikonomidis et al. 2020 and the longevity of the collected species are investigated ( Figure 5). In addition, a database for the orchids of Greece and their functional traits is, currently, under development. ...
The asymbiotic seed germination of orchids is a complex process with considerable technical
difficulties, due to the natural requirement of a plant-fungus symbiotic relationship under
field conditions. Only on rare occasions, orchid seeds are known to germinate readily in
water. Anacamptis sancta is a relatively common orchid species with its main distribution
around the East Mediterranean Sea (Greece, Cyprus, Turkey, Lebanon, Syria and Israel).
Germination of A. sancta seeds was tested in different nutrient media, water agar and under
various light regimes, in asymbiotic culture. In most experimental conditions tested, high germination
percentages were observed with the highest germination (85.5%) obtained with a
modified version of Malmgren medium without coconut water, under white light (12/12 h) at
20oC. In this study, results from all the experiments carried out are presented.
This is the second issue of the series of germination reports from Mediterranean areas (sensu Med-Checklist). It comprises germination protocols for 23 taxa: Neotinea from Greece by S. Oikonomidis & al. (No. 23); Berardia, Potentilla, Silene, and Tephroseris from North Italy by V. Carasso & al. (Nos. 24-27); Aethionema, Arenaria, Iberis, and Odontarrhena from Sicily by F. Carruggio & al. (Nos. 28-31); Astragalus, Erysimum, Senecio, Silene, and Tanacetum from Sicily by C. Salmeri & M. Castrogiovanni (Nos. 32-36); Onopordum from central Italy by S. Zitti & al. (No. 37); Viola from central Italy and Sicily by S. Magrini & L. Zucconi (Nos. 38-41); Hieracium and Pilosella from Sicily and South Italy by E. Di Gristina & al. (Nos. 42-45).
