Application of Ascophyllum nodosum-Based Soluble Extract on Micropropagation and Regeneration of Nicotiana benthamiana and Prunus domestica
Abstract
:1. Introduction
2. Results
2.1. Analysis of Plant Growth Regulators Composition of Ascophyllum Nudosum Extract
2.2. Effect of Ascophyllum Nodosum Extract on In Vitro Micropropagation of Nicotiana benthamiana
2.3. Effect of Ascophyllum nodosum Extract on Regeneration of Nicotiana benthamiana
2.4. Effect of Ascophyllum nodosum Extracts on Regeneration of Prunus domestica
3. Discussion
4. Materials and Methods
4.1. Seaweed Extracts and Plant Materials
4.2. Analysis of Plant Growth Regulators Present in Seaweed Extract
4.3. In Vitro Micropropagation Experiments
4.4. In Vitro Regeneration Experiments
4.5. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ACC | TZ | ZR | 2-iP | GA1 | GA3 | GA4 | IAA | ABA | JA | SA |
---|---|---|---|---|---|---|---|---|---|---|
NF | NF | NF | 0.47 | NF | NF | 0.11 | NF | 0.52 | NF | 10.20 |
Culture Media | Shoot Length (mm) | Shoot Diameter (mm) | Root Length (mm) | Leaf Area (mm2) |
---|---|---|---|---|
MS/2 | 25.0 ± 0.7 | 1.34 ± 0.06 | 5.3.0 ± 2.0 | 363 ± 30 |
MS/2 + 20 AE | 31.1 ± 1.5 * | 1.30 ± 0.11 | 54.0 ± 4.5 | 402 ± 39 |
MS/2 + 40 AE | 34.7 ± 1.9 * | 1.40 ± 0.12 | 61.3 ± 4.0 | 378 ± 32 |
MS/2 + 60 AE | 30.8 ± 2.3 * | 1.40 ± 0.12 | 5.67 ± 2.1 | 404 ± 43 |
MS/2 + 80 AE | 31.7 ± 1.8 * | 1.20 ± 0.07 * | 5.92 ± 4.6 | 381 ± 36 |
MS/2 + 100 AE | 24.4 ± 2.5 | 1.04 ± 0.04 * | 67.1 ± 3.7 * | 244 ± 53 * |
20 AE | 9.2 ± 1.5 * | 1.00 ± 0.03 * | 48.3 ± 6.0 | 60 ± 8 * |
40 AE | 9.2 ± 0.8 * | 1.00 ± 0.04 * | 38.3 ± 5.4 * | 61 ± 7 * |
60 AE | 10.8 ± 0.8 * | 0.96 ± 0.04 * | 25.0 ± 4.3 * | 48 ± 3 * |
80 AE | 10.0 ± 0.7 * | 1.00 ± 0.04 * | 38.3 ± 6.0 * | 59 ± 10 * |
100 AE | 11.7 ± 1.1 * | 0.92 ± 0.05 * | 38.3 ± 6.5 * | 63 ± 13 * |
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Faize, M.; Faize, L.; Burgos, L.; Critchley, A.T.; Alburquerque, N. Application of Ascophyllum nodosum-Based Soluble Extract on Micropropagation and Regeneration of Nicotiana benthamiana and Prunus domestica. Plants 2021, 10, 1354. https://doi.org/10.3390/plants10071354
Faize M, Faize L, Burgos L, Critchley AT, Alburquerque N. Application of Ascophyllum nodosum-Based Soluble Extract on Micropropagation and Regeneration of Nicotiana benthamiana and Prunus domestica. Plants. 2021; 10(7):1354. https://doi.org/10.3390/plants10071354
Chicago/Turabian StyleFaize, Mohamed, Lydia Faize, Lorenzo Burgos, Alan T. Critchley, and Nuria Alburquerque. 2021. "Application of Ascophyllum nodosum-Based Soluble Extract on Micropropagation and Regeneration of Nicotiana benthamiana and Prunus domestica" Plants 10, no. 7: 1354. https://doi.org/10.3390/plants10071354