Complete Plastome of Three Korean Asarum (Aristolochiaceae): Confirmation Tripartite Structure within Korean Asarum and Comparative Analyses
Abstract
:1. Introduction
2. Results
2.1. Plastome Features and Gene Content
2.2. Comparative Plastome Structure and Polymorphism
2.3. Repeat Sequences in the Plastome of Asarum Species
2.4. Phylogenetic Inference
3. Discussion
3.1. Structural Changes in the Plastome of Korean Asarum Species
3.2. Inferring the Molecular Phylogeny of Aristolochiaceae
3.3. Molecular Identification
4. Materials and Methods
4.1. Sampling, DNA Library Preparation, and Sequencing
4.2. Plastome Assembly and Annotation
4.3. Genome Structure and Comparative Analysis
4.4. Phylogenetic Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Scientific Name | Asarum heterotropoides | Asarum maculatum | Asarum misandrum | Aristolochia contorta |
---|---|---|---|---|
Genome length (bp) | 190,168 | 193,105 | 193,163 | 160,556 |
LSC size (bp) (=SC in Asarum) | 93,336 | 96,301 | 96,357 | 89,756 |
SSC size (bp) | - | - | - | 19,882 |
Each IR size (bp) | 48,416 | 48,402 | 48,403 | 25,459 |
Genomic structure | Tripartite (1) | Tripartite (1) | Tripartite (1) | Quadripartite |
GC contents (%) | 36.78 | 36.24 | 36.22 | 38.49 |
No. of total genes | 113 | 113 | 112 | 113 |
No. of protein-coding genes | 79 | 79 | 78 | 79 |
No. of ribosomal RNA genes | 4 | 4 | 4 | 4 |
No. of transfer RNA genes | 30 | 30 | 30 | 30 |
Group of Genes | Three Asarum Species | Aristolochia contorta |
---|---|---|
Photosystem I | psaA, psaB, psaC (×2), psaI, psaJ, ycf3 2, ycf4 | psaA, psaB, psaC, psaI, psaJ, ycf32, ycf4 |
Photosystem II | psbA, psbB, psbD, psbC, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ | psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ |
Cytochrome b6/f | petA, petB1, petD1, petG, petL, petN | petA, petB1, petD1, petG, petL, petN |
ATP synthase | atpA, atpB, atpE, atpF1, atpH, atpI | atpA, atpB, atpE, atpF1, atpH, atpI |
Rubisco | rbcL | rbcL |
NADH oxidoreductase | ndhA1 (×2), ndhB 1 (×2), ndhC, ndhD (×2), ndhE (×2), ndhF (×2), ndhG (×2), ndhH (×2), ndhI (×2), ndhJ, ndhK | ndhA1, ndhB1 (×2), ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK |
Large subunit ribosomal proteins | rpl21 (×2), rpl14, rpl16 1, rpl20, rpl22 (×2), rpl23 (×2), rpl32 (×2), rpl33, rpl36 | rpl21 (×2), rpl14, rpl16 1, rpl20, rpl22, rpl23 (×2), rpl32, rpl33, rpl36 |
Small subunit ribosomal proteins | rps2, rps3 (×2), rps4, rps7 (×2), rps8, rps11, rps12 2 (×2), rps14, rps15 (×2), rps161, rps18, rps19 (×2) | rps2, rps3, rps4, rps7, rps8, rps11, rps122 (×2), rps14, rps15, rps16 1, rps18, rps19 |
RNA polymerase | rpoA, rpoB, rpoC11, rpoC2 | rpoA, rpoB, rpoC11, rpoC2 |
Unknown function genes | ycf1 (×2), ycf2 (×2) | ycf1, ycf2 (×2) |
Other genes | accD, ccsA (×2), cemA a, clpP 2, infA, matK | accD, ccsA, cemA, clpP2, infA, matK |
Ribosomal RNAs | rrn16 (×2), rrn23 (×2), rrn4.5 (×2), rrn5 (×2) | rrn16 (×2), rrn23 (×2), rrn4.5 (×2), rrn5 (×2) |
Transfer RNAs | trnA-UGC1 (×2), trnC-GCA, trnD-GUC, trnE-UUC, trnF-GAA, trnG-GCC, trnG-UCC 1, trnH-GUG, trnI-CAU (×2), trnI-GAU 1 (×2), trnK-UUU 1, trnL-CAA (×2), trnL-UAA 1, trnL-UAG (×2), trnM-CAU, trnfM-CAU, trnN-GUU (×2), trnP-UGG, trnQ-UUG, trnR-ACG (×2), trnR-UCU, trnS-GCU, trnS-GGA, trnS-UGA, trnT-GGU, trnT-UGU, trnV-GAC (×2), trnV-UAC 1, trnW-CCA, trnY-GUA | trnA-UGC1 (×2), trnC-GCA, trnD-GUC, trnE-UUC, trnF-GAA, trnG-GCC, trnG-UCC 1, trnH-GUG, trnI-CAU (×2), trnI-GAU 1 (×2), trnK-UUU 1, trnL-CAA (×2), trnL-UAA 1, trnL-UAG, trnM-CAU, trnfM-CAU, trnN-GUU (×2), trnP-UGG, trnQ-UUG, trnR-ACG (×2), trnR-UCU, trnS-GCU, trnS-GGA, trnS-UGA, trnT-GGU, trnT-UGU, trnV-GAC (×2), trnV-UAC 1, trnW-CCA, trnY-GUA |
No. of genes | 113 (112 in A. misandrum) | 113 |
Unit Size | ||||||||
---|---|---|---|---|---|---|---|---|
Species | Mono | Di | Tri | Tetra | Penta | Hexa | C * | Total |
Asarum sieboldii | 76 | 20 | 15 | 6 | 547 | 241 | 134 | 1039 |
A. misandrum | 77 | 20 | 15 | 6 | 536 | 244 | 139 | 1037 |
A. maculatum | 78 | 18 | 12 | 6 | 536 | 243 | 135 | 1028 |
A. heterotropoides | 77 | 17 | 10 | 7 | 494 | 232 | 116 | 953 |
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Yoo, M.-J.; Jin, D.-P.; Lee, H.-O.; Lim, C.E. Complete Plastome of Three Korean Asarum (Aristolochiaceae): Confirmation Tripartite Structure within Korean Asarum and Comparative Analyses. Plants 2021, 10, 2056. https://doi.org/10.3390/plants10102056
Yoo M-J, Jin D-P, Lee H-O, Lim CE. Complete Plastome of Three Korean Asarum (Aristolochiaceae): Confirmation Tripartite Structure within Korean Asarum and Comparative Analyses. Plants. 2021; 10(10):2056. https://doi.org/10.3390/plants10102056
Chicago/Turabian StyleYoo, Mi-Jeong, Dong-Pil Jin, Hyun-Oh Lee, and Chae Eun Lim. 2021. "Complete Plastome of Three Korean Asarum (Aristolochiaceae): Confirmation Tripartite Structure within Korean Asarum and Comparative Analyses" Plants 10, no. 10: 2056. https://doi.org/10.3390/plants10102056