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Preliminary Communication

Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms

    Fernanda Lourenção Brighenti

    Faculty of Dentistry, Univ. Estadual Paulista/UNESP, Araraquara, R. Humaitá, 1680, Araraquara, SP 14801-903, Brazil

    ,
    Marcos José Salvador

    Institute of Biology, University of Campinas/UNICAMP, Postal Box 6109, Campinas, SP 3083-970, Brazil

    ,
    Aline Vidal Lacerda Gontijo

    Institute of Biology, University of Campinas/UNICAMP, Postal Box 6109, Campinas, SP 3083-970, Brazil

    ,
    Alberto Carlos Botazzo Delbem

    Faculty of Dentistry, Univ. Estadual Paulista/UNESP, Araçatuba, R. José Bonifácio, 1193, Araçatuba, SP 16015-050, Brazil

    ,
    Ádina Cléia Botazzo Delbem

    Faculty of Dentistry, Univ. Estadual Paulista/UNESP, Araçatuba, R. José Bonifácio, 1193, Araçatuba, SP 16015-050, Brazil

    ,
    Cristina Pacheco Soares

    Universidade do Vale do Paraíba – UNIVAP, Av. Shishima Hifumi, 2911, São José dos Campos, SP 12244-390, Brazil

    ,
    Maria Alcionéia Carvalho de Oliveira

    Institute of Science & Technology, Univ. Estadual Paulista/UNESP, São José dos Campos, Av. Eng. Francisco José Longo, 777, São José dos Campos, SP 12245-000, Brazil

    ,
    Camila Miorelli Girondi

    Institute of Science & Technology, Univ. Estadual Paulista/UNESP, São José dos Campos, Av. Eng. Francisco José Longo, 777, São José dos Campos, SP 12245-000, Brazil

    &
    Cristiane Yumi Koga-Ito

    *Author for correspondence:

    E-mail Address: cristiane@fosjc.unesp.br

    Institute of Science & Technology, Univ. Estadual Paulista/UNESP, São José dos Campos, Av. Eng. Francisco José Longo, 777, São José dos Campos, SP 12245-000, Brazil

    Published Online:https://doi.org/10.2217/fmb-2016-0094

    Aim: This study screened plants from Brazilian Pantanal for Candida albicans antibiofilm activity. Material & Methods: Sixty extracts were obtained from ten plants using different extraction methods. Antifungal activity was assessed. Effects on biofilm inhibition and disruption and cytotoxicity were also evaluated. The most active extract was chemically characterized. Results: Buchenavia tomentosa ethanolic extract showed noticeable antifungal activity and was selected for biofilm experiments. Subinhibitory concentration of extract inhibited fungal adhesion. Maximum killing reached 90% of C. albicans cells in suspension and 65% of cells in biofilms. The active extract was noncytotoxic. Chemical characterization showed the presence of phenols. Ellagic and gallic acids showed activity on C. albicans. Conclusion: B. tomentosa extract and its isolated compound, ellagic acid, presented antibiofilm activity and low toxicity.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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