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In vitro assessment of the anthelmintic activity of Hedysarum carnosum Desf. at different phenological stages and from six locations in Tunisia

Published online by Cambridge University Press:  03 March 2016

A. AISSA ,
F. MANOLARAKI ,
H. BEN SALEM ,
H. HOSTE  and
K. KRAIEM
A. AISSA*
Affiliation:
Institut Supérieur Agronomique de Chott-Mariem – Université de Sousse, BP 47, 4042 Chott-Mariem, Tunisie UMR 1225 INRA/ENVT, Ecole Nationale Vétérinaire de Toulouse – 23 Chemin des Capelles, 31076 Toulouse Cedex, France
F. MANOLARAKI
Affiliation:
UMR 1225 INRA/ENVT, Ecole Nationale Vétérinaire de Toulouse – 23 Chemin des Capelles, 31076 Toulouse Cedex, France
H. BEN SALEM
Affiliation:
International Center for Agricultural Research in the Dry Areas (ICARDA), DSIPS Program, 11195 Amman, Jordan
H. HOSTE
Affiliation:
UMR 1225 INRA/ENVT, Ecole Nationale Vétérinaire de Toulouse – 23 Chemin des Capelles, 31076 Toulouse Cedex, France
K. KRAIEM
Affiliation:
Institut Supérieur Agronomique de Chott-Mariem – Université de Sousse, BP 47, 4042 Chott-Mariem, Tunisie
*
*Corresponding author. Institut Supérieur Agronomique de Chott-Mariem, 38 RUE DAR SULTAN, SOUSSE 4000, Tunisie. E-mail: asaissa@laposte.net
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Summary

Gastrointestinal nematodes are compromising productivity of grazing sheep and goats. Therefore, scientists have been looking for cost-effective alternative options. Forage legumes (Fabacea Family) contain tannins that could improve livestock performance and their health as well. The present study aimed to (i) determine the in vitro anthelmintic (AH) activity of 19 acetonic extracts of Hedysarum carnosum Desf on Haemonchus contortus by a larval exsheathment assay (LEA); (ii) test the anthelmintic activity of condensed tannins using a deactivating reagent, polyvinylpolypyrrolidone (PVPP); (iii) study the effect of location and the phenological stage on the percentage of exsheathment. The LEA was used at different concentrations (150, 300, 600, 1200 µg mL−1 of acetonic extract/mL of purified buffer solution (PBS)). The larval exsheathment is concentration, location, phenological stage dependent. All extracts, caused a delay of the percentage of exsheathment over 50% so the AH activity of H. carnosum was confirmed. After addition of PVPP, the % exsheathment was similar to the 150 µg mL−1 concentration. The biplot showed that Loc1(S), Loc4(B), Loc 5(PF), Loc 6(BM) and Loc 6(PF) were isolated from other plant extract sample. Our in vitro study showed that H. carnosum seems to be a promising alternative to AH drugs.

Keywords

Hedysarum carnosum DesflocationTanninslarval exsheathment assaynematodes
Type
Research Article
Information
Parasitology , Volume 143 , Issue 6 , May 2016 , pp. 778 - 786
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Copyright
Copyright © Cambridge University Press 2016 

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