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Stability of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) isolates during repeated in vitro subculture and evaluation of an oil-in-water mycoinsecticide

Published online by Cambridge University Press:  24 May 2022

Emine Sönmez ,
Hülya Uzunoğlu ,
Ardahan Eski ,
Zihni Demirbağ  and
İsmail Demir Open the ORCID record for İsmail Demir [Opens in a new window]
Emine Sönmez
Affiliation:
1Düzce University, Environment and Health Centre, 81620, Düzce, Turkey
Hülya Uzunoğlu
Affiliation:
2Karadeniz Technical University, Faculty of Science, Department of Biology, 61080, Trabzon, Turkey
Ardahan Eski
Affiliation:
3Bilecik Şeyh Edebali University, Vocational School, Program of Biomedical Equipment Technology, 11100, Bilecik, Turkey
Zihni Demirbağ
Affiliation:
2Karadeniz Technical University, Faculty of Science, Department of Biology, 61080, Trabzon, Turkey
İsmail Demir*
Affiliation:
2Karadeniz Technical University, Faculty of Science, Department of Biology, 61080, Trabzon, Turkey
*
*Corresponding author. Email: idemir@ktu.edu.tr
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Abstract

Nine Metarhizium anisopliae (Hypocreales: Clavicipitaceae) isolates were evaluated for efficacy against Melolontha melolontha (Coleoptera: Scarabaeidae) larvae, stability in culture, and the superior isolate used to produce an oil-in-water mycoinsecticide. The phenotypic and genotypic characters of four isolates with high virulence were evaluated for their stability after repeating 12 cycles of in vitro subculture. Repeated subculture did not affect the germination of conidia; however, the morphology of some isolates changed significantly. Three isolates lost their virulence, whereas the KTU-2 isolate remained highly pathogenic. Therefore, KTU-2 was selected as the superior isolate for mycoinsecticide production. After the conidia of KTU-2 were produced by solid-state fermentation using cracked rice as the substrate, the conidia were formulated as an oil-in-water emulsion and its efficacy was assessed. The formulation caused 80% mortality on Me. melolontha larvae even at the lowest application rate (1 × 105 conidia/mL) in pot experiments, and complete mortality was obtained with the concentration of 1 × 107 conidia/mL. Lethal concentrations that kill 50% and 95% of Me. melolontha larvae present were estimated as 9.29 × 103 and 2.1 × 106 conidia/mL, respectively. Oil-in-water mycoinsecticide could be a potential candidate for the commercial control of Me. melolontha and other white grubs.

Type
Research Paper
Information
The Canadian Entomologist , Volume 154 , Issue 1 , 2022 , e26
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Copyright
© The Author(s), 2022. Published by Cambridge University Press on behalf of the Entomological Society of Canada

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Footnotes

Subject editor: Zhen Zhou

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