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2022, Pharmacia
Effects of increased concentration of calcium chloride on growth and production of flavonoids in newly established shoot and callus Gypsophila glomerata cultures were studied. The highest impact of CaCl2 on the growth index was determined in callus cultures (GI = 0.92), while in shoot cultures calcium treatment reduced the amount of biomass (GI = 0.38). Total flavonoids in shoot cultures grown on MS medium and MS medium supplemented with double amount of CaCl2 were 0.36 mg/g d. w. In both callus cultures, 2 mg/g d. w. total flavonoids were quantified. Shoots and callus grown on non-modified media accumulated 0.02 mg/g d. w. quercetin derivatives. Unlike these, both shoots and callus grown on calcium-enriched media accumulated 0.03 and 0.05 mg/g d. w. of isorhamnetin derivatives. In vitro shoot cultures grown on MS medium enriched in twice the amount of CaCl2 accumulated the highest amount of saponarin (0.138 mg/mg d. w.).
Establishment of in vitro cultures from Astragalus glycyphyllos, determination of biomass and analysis of total flavonoids, rutin and camelliaside A were performed. To increase flavonoid production various combinations of plant hormones and light/dark regimen were investigated. Suspension cultures with exogenous quercetin were evaluated for possible increase in flavonoid production. Shoots, calli and suspensions were successfully established. Rutin and camelliaside A were proved in highest amount in shoots. Calli, cultivated on modified G48 medium, with double amount of Ca2+ and Mg2+, achieved higher total flavonoid content (2.37 and 2.03 mg/g DW). Suspensions cultures, cultivated on modified G48 medium with 10, 20 and 30 mg/mL quercetin achieved higher total flavonoid content (0.09, 0.10 and 0.13 mg/mg DW). Biotransformation of quercetin to isoquercitrin was achieved. The highest concentration of isoquercitrin (56.73 ng/mg DW) was observed on suspensions cultures cultivated on modi...
Phytochemistry Reviews
Flavonoids of the Caryophyllaceae2021 •
The plant family Caryophyllaceae, commonly known as the pink family, is divided into 3 subfamilies and contains over 80 genera with more than 2600 species that are widely distributed in temperate climate zones. Plants belonging to this family produce a variety of secondary metabolites important in an ecological context; however, some of these metabolites also show health-promoting activities. The most important classes of phytochemicals include saponins, phytoecdysteroids, other sterols, flavonoids, lignans, other polyphenols, essential oils, andN-containing compounds such as vitamins, alkaloids or cyclopeptides. Flavonoids are polyphenolic compounds that remain one of the most extensively studied constituents of the Caryophyllaceae family. Numerous structurally diverse aglycones, including flavones, flavonols, flavonones (dihydroflavones), flavonols, isoflavones, and theirO- orC-glycosides, exhibit multiple interesting biological and pharmacological activities, such as antioxidant,...
Biotechnology & Biotechnological Equipment
Production of saponins from in vitro cultures of Astragalus glycyphyllos and their antineoplastic activity2021 •
Five isoflavonoids, i.e. 5-hydroxy-7-methoxy-2’, 5’-dihydroxyisoflavone (AV4), 5, 7-dihydroxy-4’-methoxyisoflavone (AV6), 7-methoxy-5-hydroxy-4’-methoxy-2’-hydroxyisoflavone (AV7), 8-pregnyl genistein (AV9), 5,7-dihydroxy-8-pregnyl-4’-methoxy-2’-hydroxyisoflavone (AV10) and one coumarochromone – sophorophenolone (AV8) were isolated from EtOAc of in vitro callus cultures of Astragalus vesicarius ssp. carniolicus, after enzymatic hydrolysis with β-glucosidase. Their structures were tentatively elucidated by spectroscopic mean (1H NMR and HR-ESI-MS spectra). Antiproliferative activity of EtOAc extract and isolated aglycones against chemosensitive human promyelocyte cell line HL-60 and its multidrug-resistant variant HL-60/Dox was assessed in vitro. Despite the strong activity of EtOAc (IC50 8.8 µg/mL (HL-6, 72 h) to 11.8 µg/mL (HL-60/Dox, 72 h)), prenylated compound AV9 showed also antiproliferative activity – 36.1 µg/mL (HL-60 and HL-60/Dox, 72 h).
Pharmacognosy Reviews
Anticancer compounds from in vitro cultures of rare medicinal plants2008 •
Malignant diseases are the second mortality cause within the human population. Due to their complex structure with several chiral centres important anticancer agents are still extracted from plants and not synthesized chemically on a commercial scale. In the coming decades, several new enabling technologies will be required to develop the next generation of advanced plantbased pharmaceuticals. This review summarizes our results concerning the production of certain anticancer compounds from tissue cultures of rare medicinal Astragalus and Linum plants. The various problems involved and the possible ways to overcome using biotechnological approaches are discussed on the basis of examples from our own research.
The aim of this study is a comparative metabolomic analysis between the endangered species Astragalus membranaceus and endemic species Astragalus thracicus concerning cycloartane saponins. In addition, in vitro shoots, callus, and suspension cultures of A. thracicus were successfully established to conserve the biodiversity of those endemic species and to increase the amount of produced saponins. The comparison was made according to the quantity of cycloartane saponins astragaloside I (1), astragaloside II (2), and astragaloside IV (4) to the reference standards for the same compounds by UHPLC-HRESI-MS analysis. The in vitro root cultures of A. thracicus reached two folds higher amounts of saponins (1.50 mg/g DW (1), 1.01 mg/g DW (2), and 0.91 mg/g DW (3)) than the native root of A. thracicus (1.14 mg/g DW (1) 0.47 mg/g DW (2), 0.40 mg/g DW (3)), and up to six times higher when compared with roots A. membranaceus (0.23 mg/g DW (1), 0.18 mg/g DW (2) and 0.05 mg/g DW (3)).
Journal of Medicinal Plants Research
A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures2009 •
2019 •
Central European Journal of Biology
Hairy roots of Hypericum perforatum L.: a promising system for xanthone production2013 •
Croatica Chemica Acta
Phenolic Compounds in Centaurea rupestris Tissues and Their Antiphytoviral Activity2014 •
Biotechnology & Biotechnological Equipment
Exploitation of the Bulgarian Flora's Biodiversity as a Source of Immunomodulatory and/or Antineoplastic Agents: Current Challenges and Perspectives2007 •
In Vitro Cellular & Developmental Biology - Plant
The impact of media composition on production of flavonoids in agitated shoot cultures of the three Hypericum perforatum L. cultivars ‘Elixir,’ ‘Helos,’ and ‘Topas’Phytochemistry Letters
Flavonol glycosides from aerial parts of Astragalus thracicus Griseb2021 •
Al-Azhar Journal of Agricultural Research
Effect of chitosan and light conditions on the production of callus biomass, total flavonoids and total phenolics in Ginkgo biloba L2021 •
The Scientific World Journal
Phenolic Profile of Dark-Grown and Photoperiod-ExposedHypericum perforatumL. Hairy Root Cultures2013 •
Asian Journal of Plant Sciences
Phytoconstituents Profile and UPLC-ESI-MS/MS Analysis of Centaurea pumilio L. Callus Culture Following Elicitation2021 •
2021 •
2011 •
Phytochemistry Reviews
Recent progress in phytochemistry, pharmacology and biotechnology of Astragalus saponins2014 •
Biotechnology & Biotechnological Equipment
Hepato-, neuroprotective effects and QSAR studies on flavoalkaloids and flavonoids fromAstragalus monspessulanusPharmacognosy Communications
The 3rd International Symposium on Medicinal Plants, Their Cultivation And Aspects of Uses2012 •
Middle-East Journal of …
Assessment of Free Radical Scavenging Activity of Crude Extracts of Some Medicinal Plants2010 •
2021 •
TURKISH JOURNAL OF BIOLOGY
The effects of nutrient and macronutrient stress on certain secondary metabolite accumulations and redox regulation in callus cultures of Bellis perennis L2016 •
2012 •
2021 •
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
In vitro/in vivo antioxidant and hepatoprotective potential of defatted extract and flavonoids isolated from Astragalus spruneri Boiss. (Fabaceae)2018 •
Natural Bio-active Compounds
Transgenic Plant Cell Cultures: A Promising Approach for Secondary Metabolite Production2019 •
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Synthesis, structural and spectral analysis of 1-(pyrazin-2-yl) piperidin-2-ol by density functional theory2015 •
Physiology and Molecular Biology of Plants
Enhancement of rhamnetin production in Vernonia anthelmintica (L.) Willd. cell suspension cultures by eliciting with methyl jasmonate and salicylic acid2020 •
Current Issues in Molecular Biology
A Study on the Safety and Effects of Amorpha fruticosa Fruit Extract on Spontaneously Hypertensive Rats with Induced Type 2 Diabetes2021 •
Journal of Genetic Engineering and Biotechnology
Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)Molecular biology reports
Effect of explant type and plant growth regulators on callus induction, growth and secondary metabolites production in Cnidium officinale Makino2018 •
Enzyme and Microbial Technology
Root in vitro cultures of six Gypsophila species and their saponin contents2010 •
Nova Biotechnologica et Chimica
Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)2021 •
Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi
RP-HPLC Analysis of Phenolic Compounds of Gypsophıla Aucherı Boıss.: Investigation of Antioxidant and Cytotoxic ActivityBulletin of the National Research Centre
Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid2019 •