Research Article Turk J Bot 34 (2010) 159-170 © TÜBİTAK doi:10.3906/bot-0912-3 Morphological, karyological and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle Tuncay DİRMENCİ1, Ekrem DÜNDAR2,*, Görkem DENİZ2, Turan ARABACI3, Esra MARTIN4, Ziba JAMZAD5 1 Balıkesir University, Necatibey Faculty of Education, Department of Biology Education, 10100, Balıkesir - TURKEY 2Balıkesir University, Faculty of Arts and Sciences, Department of Biology, 10145, Balıkesir - TURKEY 3İnönü University, Faculty of Sciences and Arts, Malatya - TURKEY 4Niğde University, Faculty of Sciences and Arts, Niğde - TURKEY 5Research Institute of Forests & Rangelands, 13185-116, Tehran, IRAN Received: 04.12.2009 Accepted: 22.02.2010 Abstract: The genus Cyclotrichium, a member of the tribe Mentheae subtribe Menthinae (Lamiaceae, Nepetoideae), was analysed with respect to morphological revision, phylogenetic analysis, and cytogenetic properties. All species of the genus were investigated for morphological characters and ITS (internal transcribed spacers) of nrDNA sequence comparison (except C. hausknechtii for ITS). Six members of the genus were also analysed for chromosome numbers. The combined results strongly suggested that Cyclotrichium is a separate genus in Nepetoideae with distinct morphological, phylogenetic, and cytogenetic characteristics. For intrageneric phylogeny of Cyclotrichium, 3 groups were recognised: 1. C. niveum; 2. C. origanifolium; and 3. the remaining 6 species. Clinopodium s.l. and Mentha appear to be most closely related to Cyclotrichium. The phylogenetic relationship of Cyclotrichium with Clinopodium s.l., Mentha, Micromeria, Melissa, and Satureja is discussed. This is the first report on the somatic chromosome numbers of 6 Cyclotrichium species and phylogenetic analysis of Cyclotrichium based on (nrDNA) ITS sequences. Key words: Cyclotrichium, Clinopodium, Satureja, ITS, phylogeny Cyclotrichium cinsinin morfolojik, karyolojik ve filogenetik analizi: tribus Mentheae bulmacasında küçük bir çözüm Özet: Bu çalışmada Mentheae tribusunun Menthinae alt tribusuna ait Cyclotrichium cinsi (Lamiaceae, Nepetoideae), morfolojik, filogenetik ve sitogenetik yönlerden analiz edilmiştir. Cinsin bütün türleri morfolojik karakterleri ve çekirdek ribozomal ITS (internal transcribed spacers) DNA dizileri açısından incelenmiş ancak C. hausknechtii tanımlandığından beri arazide hiç bulunamadığı için ITS dizi analizine katılamamıştır (sadece tip örneğinden morfolojik inceleme yapılmıştır). Cinsin altı türü karyotip analizine tabi tutulmuş ve kromozom sayıları tespit edilmiştir. Sonuçlar Cyclotrichium’un belirgin morfolojik, filogenetik ve sitogenetik özellikleriyle Nepetoideae içerisinde farklı bir cins * E-mail: dundar@balikesir.edu.tr 159 Morphological, karyological and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle olduğunu göstermiştir. Cins içi filogenetiğe bakıldığında Cyclotrichium’un üç gruba ayrıldığı görülmüştür: 1. C. niveum, 2. C. origanifolium ve 3. geriye kalan altı tür. Clinopodium s.l. ve Mentha cinsi Cyclotrichium’a en yakın olarak gözlenmiştir. Cyclotrichium’un Clinopodium s.l., Mentha, Micromeria, Melissa ve Satureja ile ilişkileri tartışılmıştır. Altı Cyclotrichium türünün somatik kromozom sayısı ve Cyclotrichium cinsinin filogenetik analizi bu çalışma ile ilk defa rapor edilmektedir. Anahtar sözcükler: Cyclotrichium, Clinopodium, Satureja, ITS, filogenetik Introduction A group of Lamiaceae that has caused much confusion over its generic boundaries is the Satureja L. complex. Briquet (1897) included a number of genera (e.g., Satureja, Micromeria Benth., Clinopodium L., Acinos Mill., and Calamintha Mill.) in Satureja s.l., while other taxonomists (Bentham, 1848; Ball, 1972; Davis, 1982; Doroszenko, 1986) preferred a narrower circumscription of taxa and kept these genera separate. The genus Cyclotrichium Manden. & Scheng. is related to these genera in the tribe Mentheae subtribe Menthinae (Lamiaceae, Nepetoideae) (Harley et al., 2004). In earlier classification, some species of Cyclotrichium were included in the genera Micromeria (sect. Piperella Benth.), Melissa L. (sect. Clinopodium), and Clinopodium (Bentham, 1834, 1848). The Cyclotrichium was first described as sect. Cyclotrichium Boiss. with 6 species in the genus Calamintha by Boissier in Flora Orientalis (1879). Later, Briquet classified it in Satureia as sect. Cyclotrichium Briq. (Briquet, 1897). Finally, Mandenova and Schengelia (1953) introduced Cyclotrichium as a separate genus with 6 species. The small genus Cyclotrichium contains 9 species distributed in Turkey, Lebanon, Iraq, and Iran. Only one species of the genus is a Mediterranean element (C. origanifolium (Labill.) Manden. & Scheng.) and it is distributed from Turkey to Lebanon, while all others are Irano-Turanian elements and are distributed nearby at the intersection of the Turkish, Iranian, and Iraqi boundaries (Figure 1). South-west Asia is a speciation centre for Cyclotrichium (Leblebici, 1974, 1982; Rechinger 1952, 1982; Davis, 1988; Güner et al., 2000). According to the latest generic description by Harley et al. (2004), all Cyclotrichium species are perennial subshrubs, hairs simple or dendroid; leaves entire or toothed; inflorescence of auxiliary cymes, sometimes very shortly pedunculate, forming terminal thyrse of 6 to many flowered, usually remote verticillasters; bracts, at least the lowest leaf-like, upper small, inconspicuous, bracteoles ± inconspicuous, smaller than to equalling calyx; calyx weakly 2-lipped, 5-lobed (3/2), lobes triangularsubulate, posterior lobes usually shorter than anterior, calyx-tube cylindrical, straight or shortly curved, 13nerved, throat hairy; corolla resupinate (Figure 2), 2lipped, 4-lobed (3/1), white or lilac, posterior (lower) lip entire or emerginate, anterior (upper) lip 3-lobed, slightly deflexed, median lobe larger, corolla tube twisted, annulate, near middle; stamens 4, ± equal, long exserted, anthers ellipsoid, thecae 2, parallel, distinct with short connective; stigma-lobes unequal, ± subulate, posterior lobe longer; disc weakly lobed; nutlets ovoid, smooth, glabrous. C. origanifolium C. niveum C. leucotrichum C. longiflorum C. glabrescens C. straussii C. haussknechtii Figure 1. Distribution map of Cyclotrichium species. 160 C. stamineum C. depauperatum T. DİRMENCİ, E. DÜNDAR, G. DENİZ, T. ARABACI, E. MARTIN, Z. JAMZAD A C B D Figure 2. Verticillasters of C. longiflorum (A); C. stamineum (B); C. niveum (C) and C. glabrescens (D). Species of Cyclotrichium are as follows (pictures are provided if available): C. depauperatum (Bunge) Manden. & Scheng., C. glabrescens (Boiss. & Kotschy ex Rech.f.) Leblebici, C. leucotrichum (Stapf. ex Rech.f.) Leblebici, C. longiflorum Leblebici (Figure 2A), C. hausknechtii (Bunge) Manden. & Scheng., C. niveum (Boiss.) Manden. & Scheng., C. origanifolium, C. stamineum (Boiss. & Hohen) Manden. & Scheng. (Figure 2B), and C. straussii (Bornm.) Rech.f. C. niveum (Figure 2C) and C. glabrescens (Figure 2D) are endemic to Turkey, while C. haussknechtii, C. straussii, and C. depauperatum are endemics of Iran. Cyclotrichium species contain aromatic essential oils and hence they are often utilised as spices. They are also consumed as herbal tea in Turkey. Local names of these plants are “dağ nanesi”, “kız otu”, “köpek nanesi”, “karabaş otu”, and “naneruhu” (Baser et al., 1996). 161 Morphological, karyological and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle Major secondary metabolites of Cyclotrichium species are isopinocamphone, terpinen-4-ol, spathulenol, menthone, thymol, carvacrol, and pulegone (Baser et al., 2001; Kilic et al., 2007). Secondary compounds based on species are as follows: C. niveum contains pulegone; C. glabrescens contains thymol and carvacrol; C. longiflorum and C. stamineum contains isopinacamphone; C. leucotrichum contains pulegone, p-mentha-3.8-diene, and t-cadinol; C. origanifolium contains isopinacomphone and menthone (Baser et al., 1994; Baser et al., 1996; Baser et al., 2001; Tepe et al., 2005; Aslan et al., 2007, Kilic et al., 2007); C. straussii contains β-caryophyllene and germacrene D (NoriSharg & Baharvand, 2006); and C. depauperatum contains cis-pinocamphone and pulegone (Sajjadi & Mehregan, 2006). Although Cyclotrichium has been established as a separate genus since 1953 (Mandenova & Schengelia, 1953), it had long been confused with Micromeria, Melissa, Clinopodium, Calamintha, Acinos, and Satureja (see above). Moreover, Calamintha and Acinos, which are closely related to Cyclotrichium, have recently been transferred into Clinopodium (Govaerts, 1999; Harley & Granda, 2000; Ryding, 2005; Bräuchler et al., 2006) although the word Calamintha has not been completely abandoned (Özhatay et al., 2009). Hence a molecular phylogenetic analysis will greatly help in the establishment of the correct classification of this complex in addition to intrageneric revision of Cyclotrichium. For this purpose, an ITS (Internal Transcribed Spacers) sequence based phylogenetic analysis covering all of the Cyclotrichium species (except C. hausknechtii, which was never recollected) along with the related genera mentioned above were conducted. Utilisation of ITS phylogeny in plants has been effective (Baldwin et al., 1995; Álvarez & Wendel, 2003) and it has been validated by numerous reports (e.g., Liston et al., 1999; Steane et al., 1999; Bellarosa et al., 2005). Cytogenetic analysis comparing the chromosome number of Cyclotrichium species and morphological revision (including C. hausknechtii from the type specimen and isotype specimens) of the genus was also conducted. 162 Materials and methods Morphological Evaluation All Cyclotrichium species were morphologically evaluated from collected specimens and/or herbarium specimens (including type specimens and isotype specimens) in ANK (Ankara University), BM (Natural History Museum, London), E (Edinburgh), G-Boiss (Geneva), GAZI (Gazi University), HUB (Hacettepe University), ISTE (İstanbul University, School of Pharmacy), ISTO (İstanbul University, Faculty of Forestry), K (Kew), W (Natural History Museum), and in WU (Wien University). Voucher information can be found in the Appendix. Genomic DNA isolation, PCR, and Sequencing Genomic DNA isolation was performed both manually as described by Dellaporta et al. (1983) and using a Plant DNeasy kit (Qiagen GmbH, Hilden, Germany). PCR was run using the published ITS primers (White et al., 1990; Sang et al., 1995) with the following protocol on a Techne Thermal Cycler (Techne, Cambridge, UK): 5 min 95 °C initial denaturation, 35 cycles of 30 s 94 °C denaturation, 30 s 50 °C annealing and 1 min 72 °C extension, followed by a 10 min final extension at 72 °C. The primers used to amplify ITS regions were also used for sequencing, which was accomplished commercially by a biotechnology company (RefGen, Ankara, Turkey). Phylogenetic Analysis Phylogenetic analysis was conducted using the programs BioEdit (Hall, 1999) and PAUP 4.0b10 (Swofford, 2001). Multiple ITS sequences for the same species collected from conditions with potential phylogenetic importance were included in the tree although no sequence difference was detected. ITS sequences of 3 species to represent each of the related genera were obtained from GenBank and their accession numbers were indicated in parentheses on the tree. Since Clinopodium s.l. is the most complex genus related to Cyclotrichium, members of this genus from the Old World (former Calamintha taxa Clinopodium betulifolium (Boiss. & Balansa) Kuntze, C. tauricolum (P.H.Davis) Govaertz, and C. grandiflorum (L.) Kuntze, and former Acinos species (Clinopodium alpinum) and from the New World (Clinopodium georgianum R.M.Harper, C. dentatum (Chapm.) Kuntze, and C. coccineum (Nutt. ex Hook.) Kuntze) were also included in the tree in addition to T. DİRMENCİ, E. DÜNDAR, G. DENİZ, T. ARABACI, E. MARTIN, Z. JAMZAD the fact that the Old World species are also new transfers from former genera (Calamintha and Acinos). For both Melissa and Acinos there was only one ITS sequence for each in the databases available. ITS sequences of Clinopodium betulifolium, Clinopodium tauricolum, and Clinopodium grandiflorum (former Calamintha taxa) were obtained through collecting specimens, PCR amplification, and sequencing as described above. Karyology Plant materials from Turkey were collected between 2005 and 2008. Vouchers have been deposited at the Balıkesir Herbarium and Biology Department of Balıkesir University, Turkey. Root tips were obtained from surface-sterilised seeds germinated for 2 days on wet filter paper for the somatic chromosome numbers. Root tips were pretreated with α- monobromonaphthalene for 16 h at 4 °C, fixed in 3:1 ethanol:glacial acetic acid for 24 h, and stored at 4 °C until use. The root tips were washed in distilled water to remove the fixative, hydrolysed in 1 N HCl for 12 min at room temperature, and stained with 2% acetoorcein for 2 h. Permanent slides were made with the standard liquid nitrogen method. The slides were dried for 24 h at room temperature and mounted in Depex. Results Revised Dichotomous Key Below is a revised dichotomous key generated in light of the characters listed in the Table, based on the Flora of Turkey and the East Aegean Islands (Davis, 1982) and the Flora Iranica (Leblebici, 1982; Rechinger, 1982), along with our morphological evaluation of both collected specimens and herbarium materials. 1. Plant densely white-tomentellous with dendroid hairs ......................................................... C. niveum 1. Plant glabrous to various hairy, glandular or not 2. Calyx conspicuously bilabiate, upper lip divided to halfway into ±recurved, triangularacuminate teeth ..................... C. origanifolium 2. Calyx sub-bilabiate, (often inconspicuously); teeth erect, triangular-lanceolate to subulate 3. Calyx glabrous or with minute glandular papillose and sessile glands, without long hairs 4. Stems 10-15 cm, canescent, leaves ovate, acute, verticillasters many flowered, bracteoles as long as calyx, calyx grabrous or with minute glandular papillose (see Discussion) .................. C. haussknechtii 4. Stems to 35 cm, hirsute-villose, leaves suborbicular, obtuse to rounded, verticillasters lax, bracteoles as long as pedicel ........................ C. depauperatum 3. Calyx glandular puberulent or glandular pruinose, sometimes with pilose or villose hairs. 5. Stems at least densely long pilose at verticillasters, eglandular or glandular, calyx densely long pilose 6. Bracteoles 2.5-5 mm, calyx 5-7 mm ............................................ C. straussii 6. Bracteoles 4.5-6 mm, calyx 8-11 mm .................................... C. longiflorum 5. Stems glandular-puberulent or glandular-pruinose with or without long hairs 7. Stems glandular puberulent, with long spreading eglandular hairs at least at nodes; lower calyx teeth 1.5-2.5 mm, not ciliate ................. C. leucotrichum 7. Stems glandular-pruinose, lacking long eglandular hairs; lower calyx teeth 2.5-4 mm, often ciliate. 8. Bracteoles 3-4 mm, linear-lanceolate, shorter than tube, calyx straight, tube with or without long hairs; teeth ciliate or not ............... C. stamineum 8. Bracteoles 5-6 mm, lanceolate, as long as or longer than tube, calyx slightly curved, without long hairs, teeth ciliate ................ C. glabrescens Phylogenetic Tree A cladogram displaying the phylogenetic position of Cyclotrichium species with respect to each other and with respect to related genera (Clinopodium s.l., Mentha, Micromeria, Satureja, Thymus, and Melissa) was constructed (Figure 3). ITS sequences for Clinopodium betulifolium, C. tauricolum and C. 163 Species / Trait Stem Leaves Bracteoles Calyx Corolla C. haussknechtii 10-15 cm, glandular pubescent, canescent ovate, 8-10 × 5-7 mm, glandular papillate, hairy as long as calyx, 5.5-7 mm, lanceolate 6.5-8 mm, scarcely bilabiate, glabrous rarely glandular, teeth lanceolate, ciliate, upper teeth 1.7-2 mm lower teeth c. 2.5 6-7 mm C. straussii 25-30 cm, hirsute-villose, glandular papillate ovate-orbicular, 10-15 × 8-14 mm, hirsute-villose 2.5-5 mm, linear-lanceolate subbilabiate, 5-7 mm, hirsute-villose with glandular papillate, teeth lanceolate, the uppers to 2 mm, the lowers c. 3 mm 10-12 mm C. depauperatum 35 cm, pubescent with short crispy hairs, sessile glands ovate-orbicular, c. 10 × 10 mm, short villose-tomentose as long as pedicel, 2-3 mm, lanceolate ±actinomorphic, 6-7 mm, without long hairs (rarely villose), glandular papillate with sessile glands, teeth 1.5-2 mm 9-11 mm C. niveum 20-50 cm, whitetomentellous, dendroid hairy, sessile glands ovate-elliptic, 8-14 × 4-9 mm dendroid hairy shorter than tube, 1.5-2 mm, linear to linear-subulate 4-6 mm, subbilabiate dendroid hairy upper teeth 0.5-1 mm, lower teeth 1-1.5 mm 7-9 mm C. origanifolium to 25 cm, hirsute or glabrous, with sessile or subsessile glands ovate to ovate-orbicular, 6-15 × (4-)5-13 mm, hirsute, with sessile glands or subsessile glands as long as calyx tube, 4-5 mm, lanceolateacuminate to subulate clearly bilabiate, 4.5-7.5 mm, slightly curved, glabrous to densely hirsute, upper teeth 0.8-1.5 mm, lower teeth, 1.2-2.5 mm 8-12 mm C. leucotrichum 15-45 cm, yellowish-green glandular-puberulent ovate, ovate-elliptic, 18-25 × 10-15 mm, glandular papillate, long hairy shorter than tube, 3.5-5 mm, lanceolate-subulate subbilabiate, 5.5-8 mm, straight, densely glandular papillate upper teeth 1-1.5 mm, lower teeth 1.5-2.5 mm 9-12 mm C. glabrescens 10-25 cm, glaucescentgreen. glandular pruinose, lacking long hairs ovate to broadly ovate, 10-23 × 8-20 mm, densely glandular papillate with sessile glands below 5-6 mm, lanceolate subbilabiate, 6.5-8 mm, slightly curved, glandular papillate, lower teeth 2.5-3 mm, upper teeth 2-2.5 mm 9-12 mm C. stamineum to 35 cm, glaucescentgreen, minutely glandular-pruinose without or sparsely long hairs ovate, 15-20 × 7-15 mm densely glandular papillate with or without sparsely long hairs ½-1 × tube, 3-4 mm, linear-lanceolate subbilabiate, 6-8 mm, straight, glandular papillate, upper teeth 1.2-2 mm, lower teeth 2-3 (3.5) mm 8-12 mm C. longiflorum to 45 cm, puberulent, sessile glands, slightly glandular papillate ovate to broadly ovate, 15-25 × 10-18 mm, without long hairs, with sessile glands shorter than tube, 4.5-6 mm, linear-lanceolate subbilabiate to bilabiate, 8-11 mm, slightly curved, densely pilose, upper teeth 2-3 mm, lower teeth 3-4 mm 10-13 mm Morphological, karyological and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle 164 Table. Morphological traits of Cyclotrichium species. T. DİRMENCİ, E. DÜNDAR, G. DENİZ, T. ARABACI, E. MARTIN, Z. JAMZAD 64 91 C. longiflorum (gu011996, 1200 m, Hakkari, TURKEY) C. longiflorum (gu011996, 600 m, Hakkari, TURKEY) 67 C. stamineum (gu011999, Mosul , IRAQ) C. origanifolium (gu011998, Niğde, TURKEY) C. origanifolium (gu011998, Antalya, TURKEY) C. niveum (gu011997, Sivas, TURKEY) 100 67 C. niveum (gu011997, Malatya, TURKEY) Clinopodium betulifolium (gu012001)* 87 100 Clinopodium tauricolum (gu012003)* 93 Clinopodium grandiflorum (gu012002)* Clinopodium alpinum (gi:30013366)** Clinopodium georgianum (gi:62997136) 100 Clinopodium dentatum (gi:62997135) 66 67 100 Cyc l o t ri c h i u m C. leuchotrichum (gu011995, Mardin, TURKEY) C. glabrescens (gu011994, 1100 m, Bitlis, TURKEY) C. glabrescens (gu011994, 1700 m, Bitlis, TURKEY) C. depauperatum (gu011993, Chaharmahal-e Bakhtiari, IRAN) C. straussii (gu012000, Chaharmahal-e Bakhtiari , IRAN) Clinopodium coccineum 100 98 Mentha spicata 100 Mentha sp. (gi: 62997144) Mentha suaveolens (gi:42556299) 94 Satureja hortensis (gi:30013368) Satureja subspicata (gi:194140359) Satureja cuneifolia (gi:194140361) 100 100 100 (gi:20379190) (gi: 111119913) Thymus daenensis (gi:211728816) Thymus persicus (gi:193228037) 100 51 94 Thymus vulgaris (gi:32966265) Micromeria hyssopifolia (gi:30011367) Melissa officinalis (gi:93735352) Rosmarinus officinalis (gi:42556303) Figure 3. The cladogram (generated from ITS sequences) displaying the phylogenetic position of Cyclotrichium. Rosmarinus officinalis was used as an outgroup. The alignment of the sequences was generated using the Clustal W algorithm (Thompson et al., 1994) and DNADist function of BioEdit program (Hall, 1999) was used to generate the tree. Percentage bootstrap values (of 1000 replicates) shown on branches of the tree were calculated by PAUP program (Swofford, 2001). Branch lengths are proportional to distances. The accession numbers of ITS sequences obtained from GenBank are indicated in parentheses. Cyclotrichium species are highlighted in a rounded rectangle. Factors with potential phylogenetic importance such as geographical distance and altitude for the same species are indicated in parentheses for each relevant taxon of Cyclotrichium (also see the Discussion). *Former Calamintha species. **Former Acinos species. Accession numbers starting with ”gu” belong to sequences that were obtained through this study. grandiflorum were generated along with Cyclotrichium specimens as described in the Materials and Methods, while all other sequences were taken from GenBank. Cyclotrichium species clearly formed a distinct branch displaying a monophyletic genus (Figure 3). Specimens of the same species collected from different geographical regions and from different altitudes displayed no ITS sequence difference. ITS sequences for C. hausknechtii were not included in the phylogenetic tree because this species has never been re-collected anywhere in the field since it was first collected. Morphological evaluation, however, has been conducted on the type specimen (in G-Boiss.) and on isotype specimens (in K and in BM). 165 Morphological, karyological and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle Karyological features The somatic chromosome numbers of 6 Cyclotrichium species, collected from different localities in Turkey, were determined for the first time. Cyclotrichium species investigated were diploid with chromosome numbers counted as 2n = 16. The basic chromosome number of the genus is x = 8. Analyses were performed in the following species: Cyclotrichium leucotrichum (T. Dirmenci 3593), C. glabrescens (T. Dirmenci 3592-b), C. origanifolium (T. Dirmenci 2178), C. niveum (T. Dirmenci 3566), C. stamineum (B. Yıldız 16935), and C. longiflorum (B. Yıldız 16922). Discussion Although Cyclotrichium has been established as a distinct genus since 1953 (Mandenova & Schengelia, 1953), it has a long confused taxonomical history involving Clinopodium s.l., Micromeria, Melissa, and Satureja (see Introduction). The Satureja L. complex that Cyclotrichium belongs to is still undergoing taxonomical changes as in the examples of Calamintha, Micromeria sect. Pseudomelissa, and Acinos’s recent transfer into Clinopodium (Govaerts, 1999; Harley & Granda, 2000; Ryding, 2005; Bräuchler et al., 2006). With this respect, revised taxonomy of these genera backed with molecular analysis is still needed. For this purpose, phylogenetic analysis of Cyclotrichium has been conducted for the first time. The Old World members of Clinopodium s.l. (former Calamintha taxa Clinopodium betulifolium, C. tauricolum, and C. grandiflorum) appeared closer to Cyclotrichium than other previously confused genera (such as Satureja, Micromeria, and Melissa) in the phylogenetic tree, suggesting its closer relation to Clinopodium s.l. The phylogenetic tree (Figure 3) clearly displays Cyclotrichium as a monophyletic genus and it is in very good accordance with morphological taxonomy. The tree also displays all the genera included as separate groups as well as Cyclotrichium, which was previously quite confused with the other mentioned genera. Hence, our phylogenetic analysis strongly supports Mandenova and Shengalia’s (1953) suggestion based on morphological taxonomy that Cyclotrichium is a distinct genus from all the others in question. 166 As for the intrageneric phylogeny, the cladogram is in very good accordance with the morphological classification. As can be seen from the dichotomous key and the detailed morphological traits table (Table), the genus splits into 3 groups: 1. C. niveum group, 2. C. origanifolium group, and 3. the remaining 6 species. C. niveum is full of white-tomentellous and dendroid hairs on all surfaces of the plant. In this respect, C. niveum is distinctly separated from all other species. C. origanifolium has a clearly bilabiate calyx with a recurved upper lip that is also distinct from all other species. The third group has a subbilabiate calyx and simple hairs in common. This is almost exactly the case in the phylogenetic tree, where the 3 distinct groups mentioned can be clearly seen (Figure 3). Morphological analysis of C. hausknechtii revealed some observations that are important for the dichotomous key. In the original description of C. hausknechtii there is no mention of a hairy calyx. Likewise, in Flora Orientalis (Boissier, 1879) and in Flora Iranica (Rechinger, 1982), it differs from the other species with its calyx having no hair. In our detailed morphological evaluation of type specimen (in G-Boiss.) and isotype specimens (in K and in BM), however, dense minute glandular papillose hairs on the calyx were observed. These hairs cannot be clearly seen on the isotype in K because the specimen is highly deformed and very little material is left. The type specimen in G-Boiss., however, has been very well preserved and all the morphological details can be clearly seen. Based on these observations, we suggest C. hausknechtii is more closely related to C. leucotrichum rather than to C. depauperatum. Therefore, to locate and collect new specimens of C. hausknechtii is essential to assess its correct systematic position as well as to finalise the necessary modifications in the key. One interesting observation based on both morphology and molecular data is that significant differences with potential phylogenetic importance (such as long geographical distance and high altitude differences) caused no difference in Cyclotrichium’s intrageneric taxonomy. Two different specimens of C. origanifolium collected from Antalya and Niğde for instance, displayed no taxonomical difference despite about 550 km distance and a different climate. T. DİRMENCİ, E. DÜNDAR, G. DENİZ, T. ARABACI, E. MARTIN, Z. JAMZAD Likewise, no taxonomical difference was observed for the 2 specimens of C. longiflorum that were collected at 600 m and 1200 m respectively, in Hakkari (Figure 3). Related to the genus Cyclotrichium, no karyological studies have been reported in the literature. This is the first record on the somatic chromosome numbers of 6 species of the genus that are natively distributed in Turkey. As in the historical confusion based on morphology, Clinopodium s.l., Mentha, Micromeria, Satureja, and Melissa are somewhat intermingled in terms of chromosome numbers. Somatic chromosome number (2n) of Clinopodium s.l. is 2n = 18, 20, 22, 24, 48 (Nilsson & Lassen, 1971; Löve & Kjellqvist, 1974; Ubera, 1979; Fernandes & Leitao, 1985; Morales, 1990, 1994), Mentha has 2n = 18, 20, 24, 36, 40, 48, 50, 72, 96, 120 (Harley & Brighton, 1977; Chambers & Hummer, 1994) Micromeria has 2n = 20, 22, 26, 30, 48, 60 (Cardona, 1973; Cardona & Contandriopoulos, 1983; Fernandes & Leitao, 1985; Morales, 1990, 1991; Luque & Lifante, 1991; Bräuchler, et al., 2008), Satureja has 2n = 30 (Löve & Kjellqvist, 1974; Lopez, 1982), and Melissa has 2n = 32 (Fernandes & Leitao, 1985). Cyclotrichium, however, has 16 somatic chromosomes (2n) and this number clearly separates this genus from all the others mentioned. In summary, this is the first report that presents a morphological and phylogenetic revision including all species of Cyclotrichium, and also suggests a different position of the genus based on chromosome numbers. Acknowledgements This research was supported by TÜBİTAK (grant number 104T293), Balıkesir University and the SYNTHESYS program (GB-TAF-3087). Special thanks to the curators of MB, E, G, K, W, WU and Turkish herbaria for letting us examine their specimens. Further thanks to E and Prof. Dr. Bayram Yıldız providing us with plant materials for DNA extraction. References Álvarez I & Wendel JF (2003). Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol 29: 417-434. Bentham G (1834). Labiatarum, genara et species. J. Ridgway and Sons, London Aslan S, Firat M & Konuklugil B (2007). Essential oil of Cyclotrichium longiflorum Leblebici. Chem Nat Comp 43: 724-725. Bentham G (1848). Labiatae. In: AP De Candolle, (ed.) Prodramus naturalis regni vegetabilis. pp. 212-226. Paris: Victoris Masson. Baldwin BG, Sanderson MJ, Porter JM, Wojciechowski MF, Campbell CS & Donoghue MJ (1995). The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Mo Bot Gard 82: 247-277. Boissier E (1879). Flora Orientalis, Vol 4, pp. 575-583. Basel/Genève: Georg. Ball PW (1972). Taxonomic and nomenclatural notes on European Labiatae (Acinos Mill., Calamintha Mill., Lamium L., Satureja L.). Bot J Linn Soc 65: 341-358. Baser KHC, Demirci B, Demirci F, Kirimer N & Hedge IC (2001). Microdistillation as a useful tool for the analysis of minute amounts of aromatic plant materials. Chem Nat Comp 37: 336338. Baser KHC, Kirimer N, Kürkçüoğlu M, Özek T & Tümen G (1996). Essential oil of Cyclotrichium origanifolium (Labill.) Manden. & Scheng. from Turkey. J Essent Oil Res 8: 569-570. Baser KHC, Sarikardasoglu S & Tümen G (1994). The essential oil of Cyclotrichium niveum (Boiss.) Manden & Scheng. J Essent Oil Res 6: 9-12. Bellarosa R, Simeone MC, Papini A & Schirone B (2005). Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp. Mol Phylogenet Evol 34: 355-370. Bräuchler C, Meimberg H & Heubl G (2006). New names in Old World Clinopodium - the transfer of the species of Micromeria sect. Pseudomelissa to Clinopodium. Taxon 55: 977-981. Bräuchler C, Ryding O & Heubl G (2008). The genus Micromeria (Lamiaceae), a synoptical update. Willdenowia 38: 363-410. Briquet J (1897). Labiatae. In: A Engler, K Prantl, (eds.) Die Natürlichen Pflanzenfamilien. Leipzig: Verlag von Wilhelm Engelmann. Cardona MA (1973). Contribution à l’étude cytotaxonomique de la flore das Baléares. Acta Phytotaxon Sin 14: 1-20. Cardona MA & Contandriopoulos J (1983). Chromosome number reports. Taxon 32: 323-324. Chambers HL & Hummer KE (1994). Chromosome counts in the Mentha collection at the USDA: ARS National Clonal Germplasm Repository. Taxon 43: 423-432. Davis PH, Mill RR & Tan K (1982). Flora of Turkey and the East Aegean Islands, Vol. 7. Edinburgh: Edinburgh University Press. 167 Morphological, karyological and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle Davis PH, Mill RR & Tan K (1988). Flora of Turkey and the East Aegean Islands (Suppl. 1). Edinburgh: Edinburgh University Press. Dellaporta SL, Wood J & Hicks JB (1983). A plant DNA minipreparation: version II. Plant Mol Biol Rep 1: 19-21. Doroszenko A (1986). Taxonomic Studies on the Satureja L. Complex (Labiatae). Edinburgh: Edinburgh University Press. Fernandes A & Leitao MT (1985). Contribution a l’étude citotaxonomique des spermatophyta du Portugal. XVIII. Lamiaceae. Mem Soc Brot 27: 27-75. Mandenova I & Schengelia E (1953). Novum genus Labiatarum Antasiaticum. Not Syst 15: 332-337. Morales R (1990). Números cromosomáticos de plantas occidentales. Ann Jard Bot Madrid 47: 193-198. Morales R (1991). El género Micromeria Bentham (Labiatae) en la Península Ibérica e islas Baleares. Ann Jard Bot Madrid 48: 131156. Morales R (1994). Números cromosómicos para la Flora Española. Lagascalia 17: 388-391. Govaerts R (1999). World checklist of seed plants - the species, Vol 3, pp. 15-19. Antwerp: Book & Media Publ. Nilsson O & Lassen P (1971). Chromosome numbers of vascular plants from Austria, Mallorca and Yugoslavia. Bot Not 124: 270276. Güner A, Özhatay N, Ekim T & Baser KHC (2000). Flora of Turkey and the East Aegean Islands (Suppl. 2). Edinburgh: Edinburgh University Press. Nori-Shargh D & Baharvand B (2006). Volatile constituents analysis of Cyclotrichium straussii Bornm. from Iran. J Essent Oil Res 18: 261-262. Hall TA (1999). BioEdit: a user-friendly biological sequence alignment editor and analyses program for Windows 95/98/NT. pp. 95-98. In: Nucleic Acids Symp. Özhatay N, Kültür Ş & Aslan S (2009). Check-list of additional taxa to the supplement Flora of Turkey IV. Turk J Bot 33: 191-196. Harley RM & Brighton CA (1977). Chromosome numbers in the genus Mentha L. Bot J Linn Soc 74: 71-96. Harley RM, Atkins S, Budantsev AL, Canlino PD, Conn BJ, Grayer R, Harley MM, De Kok R, KresLovskaja T, Morales R, Palon AJ, Ryding O & Upson T (2004). Labiatae. In: JW Kadereit, (ed.) The Families and Genera of Vascular Plants, VII, Flowering Plants-Dicotyledons, Lamiales, except Acanthaceae including Avicenniaceae. pp. 167-229. Berlin: Springer-Verlag. Harley RM & Granda AP (2000). List of species of tropical American Clinopodium (Labiatae), with new combinations. Kew Bull 55: 917-927. Kilic T, Karahan A, Dirmenci T, Arabaci T, Kocabas E & Gören AC (2007). Essential oil compositions of some species Cyclotrichium and antimicrobial activities. Chem Nat Comp 43: 733-735. Leblebici E (1974). The Calaminthoid genera in Turkey: New names in Acinos and Cylotrichium, including a new species from N. Iraq. Bitki 1: 403-408. Leblebici E (1982). Cyclotrichium (Boiss.) Manden. & Scheng. In: PH Davis, (ed.) Flora of Turkey and the East Aegean Islands, Vol. 7. pp. 346-349. Edinburgh: Edinburgh University Press. Liston A, Robinson WA, Pinero D & Alvarez-Buylla ER (1999). Phylogenetics of Pinus (Pinaceae) based on nuclear ribosomal DNA Internal transcribed spacer region sequences. Mol Phylogenet Evol 11: 95-109. Lopez G (1982). Conspectus saturejarum ibericarum cum potioribus adnotationibus ad quasdam earum praesertim aspicientibus. Ann Jard Bot Madrid 38: 361-415. Löve A & Kjellqvist E (1974). Cytotaxonomy of Spanish plants. IV.Dicotyledons: Caesalpinacea-Asteracea. Lagascalia 4: 153-211. Luque T & Lifante DZ (1991). Chromosome numbers of plants collected during Iter Mediterraneum I in the SE of Spain. Bocconea 1: 303-364. 168 Rechinger KH (1952). Calamintha leucotricha Stapf., Cyclotrichium glabrescens Boiss. & Kotschy. Oesterreichische Bot Zeit 10: 6264. Rechinger KH (1982). Flora Iranica, Vol. 150. Graz: Verlagsanstalt. Ryding O (2005). Revision of the Clinopodium abyssinicum group (Labiatae). Bot J Linn Soc 150: 391-408. Sajjadi E & Mehregan I (2006). Chemical composition of the essential oil of Cyclotrichium depauperatum. Chem Nat Comp 42: 294295. Sang T, Crawford DJ & Stuessy TF (1995). Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: Implications for biogeography and concerted evolution. Proc Natl Acad Sci 92: 6813-6817. Steane DA, Scotland RW, Mabberley DJ & Olmstead RG (1999). Molecular systematics of Clerodendrum (Lamiaceae): ITS sequences and total evidence. Am J Bot 86: 98-107. Swofford DL (2001). PAUP. Phylogenetic Analysis Using Parsimony (and other methods), Version 4. Sinauer. Tepe B, Sokmen M, Sokmen A, Daferera D & Polissiou M (2005). Antimicrobial and antioxidative activity of the essential oil and various extracts of Cyclotrichium origanifolium (Labill.) Manden. & Scheng. J Food Eng 69: 335-342. Ubera JL (1979). Números cromosómicos para la Flora Española. Lagascalia 9: 123-125. White T, Bruns T, Lee S & Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M Innis, D Gelfald, J Sninsky, T White, (eds.) PCR Protocols: A Guide to Methods and Applications. pp. 315-322. San Francisco: Academic Press. T.Dirmenci, E. DÜNDAR, G. DENİZ, T. ARABACI, E. MARTIN, Z. JAMZAD Appendix Voucher specimens of the genera Cyclotrichium Manden. & Scheng. and Clinopodium s.l. Mill., which are examined in the present study. *specimens that were used for genomic DNA extraction. Cyclotrichium depauperatum: Iran Iter Syriaco-Armeniacum, Bors valley fl. Chyrsan (Khersan), 3000 ped. 08.1868, Hausskn. s.n. (holotype G, isotype K, BM). Chaharmahal-e Bakhtiari, Dareh Bazoft, Kuh-e Keynu, 2150-2400m, Mozaffarian 58000* (TARI). Cyclotrichium glabrescens: Turkey B8 Muş: Muş in subalpinis, c. 2000 m, 09.09.1859, Kotschy 1859: Suppl. 1688 (isotype G, K). Diyarbakır: Hazro, Uzunargıt, around Değirmen, stream banks and rocky slopes, 890-930 m, 03.08.1974, H.Demir, S.Akkuş, H.Olgaç (Dicle Ün. Herb.). B9 between Bitlis and Baykan 26 km, N 38º 14´860´´, E 041º 57´ 329´´, ca. 1100 m, 07.08.2007, Dirmenci 3592-b. Bitlis: Hizan, Karbastı village, Yamaç, 1500 m, 24.07.2002, T.Dirmenci 1440*. Between Bitlis and Baykan 26 km, N 38º 14´ 860´´, E 041º 57´ 329´´, ca. 1100 m, 27.06.2007, T.Dirmenci 3441*; ibid., 07.08.2007, T.Dirmenci 3592-b & M. Fırat. Şeyh Habib Mt., 1700 m, 05.08.2002, A.Altıok 2759 (VANF). Kurtboğan hill, 1650 m, 08.08.2001, A.Altıok 1756 (VABF). Around Karınca Karakolu, 1300 m, A.Altıok 1539 (VANF). ibid., 1300 m, 15.07.2001, A.Altıok 1536 (VANF). Bitlis: Hizan, Karbastı village, Gerzemel Mountain, rocky places, 16001700 m, 17.07.2001, Dirmenci 1440. Cyclotrichium haussknechtii: Iran: in fissuris rup. Teng Tokab, Nekbehan, 3000 ped., 06.1868, Hausskn. s.n. (holotype G, isotype K, BM). Cyclotrichium leucotrichum: Turkey C8 Mardin: Richemil (Rişmil), 23.07.1888, Sintenis 1352 (isotype E). Mardin: 20 km from Mardin to Diyarbakır, S. of Sultan Şeyhmus village, 15.09.2007, T.Dirmenci 3592. between Kızıltepe and Mardin, 1500 m, 23.07.1974, M.Koyuncu 4466 (AEF, GAZI, E). between Mardin and Kızıltepe, 1 km, calcareous rocky place, 22.07.2005, Arabacı 2139*; ibid., rocky slopes, ca. 1000 m, 15.09.2007, T.Dirmenci 3593. Mardin: Karaman village, 24.07.1970, T.Baytop, ISTE 18228 (E). Batman: 2 km from Gerçüş to Mardin, rocky slopes, 15.09.2007, T.Dirmenci 3594. 3 km from Hasankeyf to Mardin, rocky slopes, 16.09.2007, T.Dirmenci (observation). Cyclotrichium longiflorum: Iraq: Mosul: ad confinae Turciae prov. Hakkari, in ditione pagi Sharanish, in montibus calc. a Zakho, septentrionem versus. Jabal Khantur, in saxosis, 1200 m. 4-9.07.1957, K.H.Rechinger 10794 (holotype E, isotype G, W). Mosul: ad confinae Turciae prov. Hakkari, in ditione pagi Sharanish, in jugo montis Zawita, ca. 1800 m, 09.07.1957, KH.Rechinger 11992 (E, G, W). Mosul: ad confinae Turciae prov. Hakkari, in ditione pagi Sharanish, in jugo montis calc. a Zakho, septentrionem versus, in saxosis faucium supra marsis, ca. 1200 m, 09.07.1957, KH.Rechinger 10891 (E, G, W). Mosul: In fissures rupium calc. Septentr. Versus expositis supra pagum Basingera, ca 1200 m, 09.07.1957, K.H.Rechinger 11512 (W). Turkey C9 Hakkari: between Hakkari and Çukurca, 13 km, 1200 m, 17.06.2004, Dirmenci 2476*; ibid., 15 km, rocky places, c. 1200 m, 09.06.2006. Dirmenci 3356. Şırnak: 60 km from Şırnak to Hakkari, 600 m, 08.06.2002, Yıldız 15158*, Dirmenci & Arabacı. 63 km from Şenoba to Hakkari, rocky slopes, 15.07.2001, Z.Aytaç 8171 (ESSE, GAZI, ANK). Cyclotrichium niveum: Turkey B6 Malatya: in Cappadocia centrali inter Ketche-Mesera et Guruno (Gürün), Tchihatcheff 1854:561 (holotype GBoiss.). B6 Sivas: Gürün, Barsakdere, 1500-1600 m, 29.07.1992, Yıldız 9902*. Divriği, kale çevresi, hareketli yamaç, kalker, 1100 m, 08.09.1994, A.A.Dönmez (4270) & Z.Yeşilyurt (GAZI). 20 km from Gürün to Darande, 23.08.1991, M.Kara (ESSE 9228). Malatya: between Malatya and Darende, 1 km west of Develi village, c. 1600 m, N 38º 23´ 184´´, E 037º 54´ 571´´, 10.08.2007, Dirmenci 3506 & Arabacı. Between Gürün and Darende, 15 km, 1570 m, 11.07.2008, Z.Aytaç (9094) et al., (GAZI). Malatya/Sivas: between Gürün-Darende, rocky slopes, 1600 m, 28.06.1974, K.Karamanoğlu & M.Koyuncu (AEF-4532). Sivas: Deli Da., Bornm. 1893:3482 (E, K). Malatya: between Gürün and Malatya, 65 km from Malatya, c. 1500 m, 07.08.1956, McNeil 450 (E). Gürün, 40 km from Malatya, c. 1400 m, McNeill 466 (E, K). Malatya: between Malatya and Darende, South of Develi village, rocky place, 1400 m, 20.07.2005, Arabacı 2137*. Doğanşehir, between Erkenek and Alıçlı, 28.07.1987, Aktoklu 855 (HUB). Darande, kayalıklar, 12001800 m, A.Rıza Gürgen (AEF-17918). Between Malatya and Darende, 1 km E of Develi village, N 38 23 184, E 037 54 571, 4795 ft., 10.08.2007, T.Dirmenci 3504 & T. Arabacı. B7 Erzincan: Kemaliye (Egin) Sarıkonaklar village, 1800 m, 10.07.1982, M.Koyuncu et al., (AEF-23266). Kemaliye (Egin) around of Sarıkonaklar village, 1400 m, 09.07.1982, M.Koyuncu (5780) et al., (AEF-10532). Kemaliye (Egin), Ariki Da., 07.07.1977, O.Soner (AEF-6166). Kemaliye, Aşağı Umutlu, Gökseki Da., 1500-2400 m, M.Tanker & M.Çoşkun, 26.09.1984 (AEF-14675). Kemaliye (Egin), Kotachan, in dedivil. Lapidosis, 05.07.1890, Sintenis 2901 (K). Kemaliye, around of Sandıkbağı, 900 m, 17.11.1980, Ş.Yıldırımlı 4168 (HUB). Kemaliye, Başpınar, Buğdaypınar, 1000-1200 m, Ş.Yıldırımlı 4225 (HUB). Adıyaman: Gölbaşı, Hamzaköy, 1060 m, 15.09.2001, A.Dönmez 10127 (HUB). 169 Morphological, karyological and phylogenetic evaluation of Cyclotrichium: a piece in the tribe Mentheae puzzle Cyclotrichium origanifolium: Turkey C2 Denizli: d. Acıpayam, Bozdağ, 1670 m, 16.07.1947, Davis 13404 (E). C3 Isparta: Selçuklu, Çimenova, W side of Sang Da., 18.07.1949, Davis 15591 (E). Isparta: Sütçüler, Dedegöl Da., 2200 m, 02.08.1949, Davis 15993 (E). Antalya, Kemer, Akdağ, 1600 m, 10.07.1949, Davis 15111 (E). C3 Antalya: Akdağ, nr. Karabuynus Y., 2000 m, 31.08.1947, Davis 14533 (E). C3 Antalya: Tahtalı Da., 2100 m, 16.08.1947, Davis 14144 (E). C4 Antalya: Gebiz, west side of Bozburun Mountain, 1800-1900 m, 12.07.2002, Dirmenci 1949*. C5 İçel: Arslanköy, Bolkar Da., Gökkol Yayla, 2400 m, calcareous, 07.08.2002, Dirmenci 2178 & Yıldız. C5 Niğde: Çamardı, Aladağ, road of Yedigöller, c. 2000-2200 m, 04.08.2006, Yıldız 16443* & Dirmenci. C4 Antalya: Akdağ, S. of Geyik Da., above Gözübüyük yayla, 2200 m, 28.08.1947, Davis 14327 (E). C4 Antalya: distr. Elmalı, Üçkuyular, 1750 m, 28.07.1960, Khan et al. 274 (E). C5 Mersin: Anamur, Olucak, between Ermenek and Anamur, 18.08.1949, Davis 16334B (E). C5 Adana: Karaisalı, Bulgar Da., between Pozantı and Meydan, 1500 m, 01.09.1949, Davis 16585 (E). C5 Niğde: Regione alpine du Taurus, Pres de GülekMaden, 16.08.1855, Balansa 500 (E). C5 Niğde: Bulgar Maden, 1500 m, 07.1912, Shie 283 (E), Bulgar maden, 1400-2000 m, Shie 1896:586 (E). C5 Niğde: S valley of Maden, 30.07.1969, Darrah 348 (E). C6 Osmaniye: Amonos, Düldül, 1500-2000 m, 07.1911, Haradj. 3846 (E). 170 Cyclotrichium stamineum: Iraq in rupestribus umbrosis montis Gara, Kotschy 1841:311 (holotype G, isotype K). in rupestribus m. Gara, regione alpinam, 24.07.1841, Hohanacker s.n. (K). Sarsang, Gara Dagh, 1050-1250 m, 12.06.1958, E.Chapman 26409 (K). Mosul: Zawita, 21 km a Dohuk orientem versus in pinetis (P. brutia) saxosis, 800 m, 1012.07.1957, Rechinger 11546 (G, K, W). 25 km a Dohuk c. 800 m, Rechinger 11541 (G, W). Inter Dohuk et Amadiye, Amadiye 3 km occidentem versus, 1000 m, 12.07.1957, Rechinger 11618 (G, W). Galli Ali beg, 500 m, 20.08.1958, Ali Rawi 26795 (K). Bekhal, mountain slope, 07.07.1971, S.Omar et al., 38408 (K). Mosul, Zawita gorge, rocky crevices in Pinus brutia forest, 23.07.1961, Agnew 733 (E). Sirsang, 4000 ft, 06.07.1955, R.H.Heines 447 (E). Mosul: Sirsang, Gara mt. 10.07.1961, Agnew 648 (E). Mosul: ad confinae Turciae prov. Hakkari, inter Dohuk et Amadiya, Zawita, 21 km a Dohuk orientem versus, in fissirus dopium dolomit, c. 800 m, 12.07.1952, K.H.Rechinger 11541* (E, K). Amadiya, Zawita gorge, 23.07.1961, Agnew 733 (E). Turkey: C9 Siirt: Eruh, Yassıdağ (Serikur Da.,) Meşindağ pass (Birini pass), 1640 m, 18.07.1981, E.Tuzlacı (ISTO-47340). Hakkari: 2 km from Çukurca to Narlı village, 1200 m, 09.06.2006, T.Dirmenci 3357a. ibid., 17.08.2008, Yıldız 16935, Dirmenci & Fırat. 5 km W of Çukurca, c. 1000 km, 09.06.2006. T.Dirmenci 3358. Çukurca, 1200 m, rocky slopes in open Quercus sp. forest, 2.06.1966, Davis 44745 (ISTO-11473, E). C10 Hakkari: Cilo Da. in Diz dere, 5700 ft, gravel terraces, 06.08.1954, Davis 23924 (ANK, E).Yüksekova: nr. Oramar (S. of Cilo Da.) 1830 m, Trelawny 1845 (E). Cyclotrichium straussii: Iran: Persia occident., in ditione oppidi Sulatanabad, Strauss s.n. (isotype K, BM). Luristan, left bank of Kashgan Rud: above Pul-i-Khalor, 60 km W. of Khoramabad: dryish ledges NW of facing limestone cliffs, c. 1130 m, 11.07.1966, J.C.Archibald 2645* (E). Lorestan, Shrab-Tak, 2300-2500 m, JuneJuly, M.S.Mossadegh 44 (E). Chaharmahal-e Bakhtiari, Lordegan, Kuh-e Karkunji, 1500-2300 m, Mozaffarian 54939 (TARI). Clinopodium betulifolium: Turkey C5 İçel: above Gözne, calcareous, c. 1200 m, N 37º 00´ 26´´, E 34º 34´ 70´´, 07.08.2007, Dirmenci 3487*, Arabacı & Brauchler. Clinopodium grandiflorum: Turkey A8 Rize: c. 15 km from İkizdere to Başköy, 1260 m, 01.09.2008, Dirmenci 3600* & Akçiçek. Clinopodium tauricolum: Turkey C5 İçel: between Gülnar and Ermenek, around Güneşli village, Cedrus libani frost, 1200 m, 07.08.2007, Dirmenci 3483*, Arabacı & Brauchler