Abstract
Cyclotides are small circular peptides carrying an array of interesting biological activities and also showing interesting features for storage and bioavailability. Here, an optimized method to isolate cyclotides from two species of Psychotria, P. brachyceras and P. leiocarpa, that can be integrally performed (to isolate cyclotides) or used in part (to obtain cyclotide-rich extracts), is described. In general this protocol can be applied for cyclotide isolation from any species, taking into account potential minor adaptations for the particularities of specific cases.
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Acknowledgements
The author is grateful to several members of the Institute for Molecular Bioscience (The University of Queensland): Prof. David Craik and Aaron Poth for advice and access to research facilities, as well as Alun Jones, Olivier Cheneval, and Yen-Hua Huang for technical assistance with analytical equipment. The author is thankful to Prof. Arthur Fett-Neto (Federal University of Rio Grande do Sul) for doctoral supervision. The author was supported by the Council for Scientific and Technological Development (CNPq) (grant 306079/2013-5) and the Committee for Improvement of Higher Education Personnel (CAPES) (scholarship registration 99999.009943/2014-05).
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Nitta Matsuura, H. (2022). Cyclotide Isolation from Psychotria brachyceras and Psychotria leiocarpa. In: Fett-Neto, A.G. (eds) Plant Secondary Metabolism Engineering. Methods in Molecular Biology, vol 2469. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2185-1_14
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DOI: https://doi.org/10.1007/978-1-0716-2185-1_14
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2184-4
Online ISBN: 978-1-0716-2185-1
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