Biotechnological Studies on Nasturtium officinale (Watercress): an Endangered Species of Significant Relevance in Medicine, Cosmetic, and Food Industries

Klimek-Szczykutowicz, Marta; Ekiert, Halina; Szopa, Agnieszka

doi:10.1007/978-981-19-9936-9_22

Marta Klimek-Szczykutowicz^4,5,
Halina Ekiert⁶ &
Agnieszka Szopa⁶

Part of the book series: Sustainable Development and Biodiversity ((SDEB,volume 33))

353 Accesses

Abstract

The chapter discusses the importance of plant biotechnology research on Nasturtium officinale (watercress), an endangered species of valuable pharmaceutical, cosmetic, and nutritional properties.

This is a comprehensive study focusing on the development of N. officinale in vitro cultivation methods with the aim of stimulating the production of glucosinolates (GSLs) and polyphenols—compounds that are important from the perspective of plant utility.

In addition, optimization of N. officinale in vitro culture conditions is discussed in detail, e.g., influence of plant growth regulators (PGRs) (cytokinins and auxins), LED light wavelength spectrum, and duration of growth periods on the production of bioactive metabolites in agar and agitated N. officinale microshoot cultures.

Furthermore, the antioxidant profile of the tested in vitro culture extracts is evaluated using the following assays: cupric reducing antioxidant capacity (CUPRAC), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing ability of plasma (FRAP).

The highest total GSL content was achieved in the agar-grown microshoots over 20 days on the Murashige and Skoog (MS) medium containing 1 mg/L 6-benzyladenine (BA) and 1 mg/L 1-naphthylacetic acid (NAA) under a combination of 50% green, 35% red, and 15% blue lights (RBG). The highest total polyphenol content was detected in agar cultures cultivated over 10 days on the MS medium with 1 mg/L zeatin (Zea) and 1 mg/L NAA. The experimental cultures showed a high antioxidant potential. The optimal antioxidant activity was found for the extracts of N. officinale agitated microshoot cultures grown for 20 days on the MS medium containing 1 mg/L kinetin (KIN) and 1 mg/L 3-indolylacetic acid (IAA), as determined using the abovementioned methods.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Chapter: USD 29.95; Price excludes VAT (USA)

eBook: USD 189.00; Price excludes VAT (USA)

Hardcover Book: USD 249.99; Price excludes VAT (USA)

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Abbreviations

2,4-D:: 2,4-Dichlorophenoxyacetic acid
2iP:: 6-(γ,γ-Dimethylallylamino)purine
Ala:: Alanine
B:: Blue light (λ = 430 nm)
BA:: 6-Benzyladenine
CUPRAC:: Cupric reducing antioxidant capacity
D:: Darkness (no light)
DM:: Dry mass
DPPH:: 1,1-Diphenyl-2-picrylhydrazyl
DW:: Dry weight
EFSA:: European Food Safety Authority
F-C:: Folin-Ciocalteu
FRAP:: Ferric reducing ability of plasma
FW:: Fresh weight
GAL:: Gallic acid equivalent
GSLs:: Glucosinolates
IAA:: 3-Indolylacetic acid
IBA:: 3-Indolylbutyric acid
Ile:: Isoleucine
IPA:: 3-Indolylpyruvic acid
IUCN:: International Union for Conservation of Nature
KIN:: Kinetin
LED:: Light-emitting diodes
Leu:: Leucine
Met:: Methionine
MS:: Murashige and Skoog
NAA:: 1-Naphthylacetic acid
PGRs:: Plant growth regulators
Phe:: Phenylalanine
PPFD:: Photosynthetic photon flux density
R:: Red light (λ = 670 nm)
RB:: A combination of 70% red light and 30% blue light
RBfR:: A combination of 50% far red (λ = 730 nm), 35% red, and 15% blue
RBG:: A combination of 50% green (λ = 528 nm), 35% red, and 15% blue light
RBUV:: A combination of 50% UV-A (λ = 400 nm), 35% red, and 15% blue light
RBY:: A combination of 50% yellow (λ = 600, 630 nm), 35% red, and 15% blue light
SIN:: Sinigrin equivalent
TDZ:: Thidiazuron
TE:: Trolox equivalent
TIS:: Temporary immersion system
Trp:: Tryptophan
Tyr:: Tyrosine
Val:: Valine
WF:: White fluorescent light (λ = 390–760 nm)
WLED:: White LED light (33% warm color, 2700 K; 33% neutral color, 4500 K; 33% cold color, 5700 K)
Zea:: Zeatin

References

Afsharypuor S, Salehi M (2008) Volatile constituents of leaves and stems of Nasturtium officinale R. Br. J Essent Oil Res 20(6):517–518. https://doi.org/10.1080/10412905.2008.9700076
Article CAS Google Scholar
Ahmad A, Ahmad N, Anis M, Alatar AA, Abdel-Salam EM, Qahtan AA, Faisal M (2021) Gibberellic acid and thidiazuron promote micropropagation of an endangered woody tree (Pterocarpus marsupium Roxb.) using in vitro seedlings. Plant Cell Tissue Organ Cult 144(2):449–462. https://doi.org/10.1007/s11240-020-01969-1
Article CAS Google Scholar
Amiri H (2012) Volatile constituents and antioxidant activity of flowers, stems and leaves of Nasturtium officinale R. Br. Nat Prod Res 26(2):109–115. https://doi.org/10.1080/14786419.2010.534998
Article CAS PubMed Google Scholar
Arnold DL, Flegmann A, Clarkson JM (1995) Somaclonal variation in watercress for resistance to crook root disease. Plant Cell Rep 14(4):241–244. https://doi.org/10.1007/BF00233641
Article CAS PubMed Google Scholar
Arumugam A, Din Ibrahim M, Kntayya SB, Ain NM, Iori R, Galletti S, Ioannides C, Razis AFA (2020) Induction of apoptosis by gluconasturtiin-isothiocyanate (GNST-ITC) in human hepatocarcinoma HepG2 cells and human breast adenocarcinoma MCF-7 cells. Molecules 25(5):1240. https://doi.org/10.3390/molecules25051240
Article CAS PubMed PubMed Central Google Scholar
Asadi-Samani M, Moradi M-T, Mahmoodnia L, Alaei S, Asadi-Samani F, Luther T (2017) Traditional uses of medicinal plants to prevent and treat diabetes; an updated review of ethnobotanical studies in Iran. J Nephropathol 6(3):118–125. https://doi.org/10.15171/jnp.2017.20
Article PubMed PubMed Central Google Scholar
Ayuso M, García-Pérez P, Ramil-Rego P, Gallego PP, Barreal ME (2019) In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds. Plant Cell Tissue Organ Cult 138(3):427–435. https://doi.org/10.1007/s11240-019-01638-y
Article CAS Google Scholar
Bach A, Kapczyńska A, Dziurka K, Dziurka M (2015) Phenolic compounds and carbohydrates in relation to bulb formation in Lachenalia “Ronina” and “Rupert” in vitro cultures under different lighting environments. Sci Hortic 188:23–29. https://doi.org/10.1016/j.scienta.2015.02.038
Article CAS Google Scholar
Bahramikia S, Yazdanparast R (2008) Effect of hydroalcoholic extracts of Nasturtium officinale leaves on lipid profile in high-fat diet rats. J Ethnopharmacol 115(1):116–121. https://doi.org/10.1016/j.jep.2007.09.015
Article PubMed Google Scholar
Basch E, Bent S, Foppa I, Haskmi S, Kroll D, Mele M, Szapary P, Ulbricht C, Vora M, Yong S, Natural Standard Research C (2006) Marigold (Calendula officinalis L.): an evidence-based systematic review by the Natural Standard Research Collaboration. J Herb Pharmacother 6(3):135–159. https://doi.org/10.1300/J157v06n03
Article PubMed Google Scholar
Baskaran P, Jayabalan N (2009) In vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture. Acta Physiol Plant 31(6):1119–1127. https://doi.org/10.1007/s11738-009-0330-3
Article CAS Google Scholar
Baskaran P, Jayabalan N, van Staden J (2011) Production of psoralen by in vitro regenerated plants from callus cultures of Psoralea corylifolia L. Plant Growth Regul 65(1):47–54. https://doi.org/10.1007/s10725-011-9573-2
Article CAS Google Scholar
Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76
Article CAS PubMed Google Scholar
Blažević I, Montaut S, Burčul F, Olsen CE, Burow M, Rollin P, Agerbirk N (2020) Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants. Phytochemistry 169:112100. https://doi.org/10.1016/j.phytochem.2019.112100
Article CAS PubMed Google Scholar
Blios MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 26:1199–1200
Article Google Scholar
Blumenthal M, Busse WR, Goldberg A, Gruenwald J, Hall T, Riggins CW, Rister RS (1999) The complete German Commission E monographs. Therapeutic guide to herbal medicines. American Botanical Council and Integrative Medicine Communications, Austin
Google Scholar
Boligon AA, Janovik V, Boligon AA, Pivetta CR, Pereira RP, Da Rocha JBT, Athayde ML (2013) HPLC analysis of polyphenolic compounds and antioxidant activity in Nasturtium officinale. Int J Food Prop 16(1):61–69. https://doi.org/10.1080/10942912.2010.528111
Article CAS Google Scholar
Boyd LA, McCann MJ, Hashim Y, Bennett RN, Gill CIR, Rowland IR (2006) Assessment of the anti-genotoxic, anti-proliferative, and anti-metastatic potential of crude watercress extract in human colon cancer cells. Nutr Cancer 55(2):232–241. https://doi.org/10.1207/s15327914nc5502
Article CAS PubMed Google Scholar
Camponogara C, Silva CR, Brusco I, Piana M, Faccin H, de Carvalho LM, Schuch A, Trevisan G, Oliveira SM (2019) Nasturtium officinale R. Br. effectively reduces the skin inflammation induced by croton oil via glucocorticoid receptor-dependent and NF-κB pathways without causing toxicological effects in mice. J Ethnopharmacol 229:190–204. https://doi.org/10.1016/j.jep.2018.10.011
Article CAS PubMed Google Scholar
Casanova NA, Ariagno JI, López Nigro MM, Mendeluk GR, Gette de los MA, Petenatti E, Palaoro LA, Carballo MA (2013) In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage. J Appl Toxicol 33(9):880–885. https://doi.org/10.1002/jat.2746
Article CAS PubMed Google Scholar
Centers for Disease Control and Prevention (CDC). https://www.cdc.gov/. Accessed 31 Mar 2022
Claxton JR, Arnold DL, Clarkson JM, Blakesley D (1998) The regeneration and screening of watercress somaclones for resistance to Spongospora subterranea f. sp. Nasturtii and measurement of somaclonal variation. Plant Cell Tissue Organ Cult 52:155–164
Article CAS Google Scholar
Daneshvar Royandazagh S (2019) Efficient approaches to in vitro multiplication of Lilium candidum L. with consistent and safe access throughout year and acclimatization of plant under hot-summer Mediterranean (Csa Type) climate. Not Bot Horti Agrobot Cluj-Napoca 47(3):734–742. https://doi.org/10.15835/nbha47311486
Article CAS Google Scholar
De Souza DA, Costa PM, Ribeiro RIMA, Vidigal PVT, Pinto FCH (2016) Daily intake of watercress causes inhibition of experimental Ehrlich tumor growth. J Bras Patol Med Lab 52(6):393–399. https://doi.org/10.5935/1676-2444.20160063
Article CAS Google Scholar
Debnath M, Pandey M, Chikara SK (2011) Physiological and molecular characterization of in vitro cultures of an endemic medicinal herb, Chlorophytum borivilianum, under abiotic stress. Afr J Biotechnol 10(38):7356–7366. https://doi.org/10.4314/ajb.v10i38
Article CAS Google Scholar
Erişen S, Kurt-Gür G, Servi H (2020) In vitro propagation of Salvia sclarea L. by meta-topolin, and assessment of genetic stability and secondary metabolite profiling of micropropagated plants. Ind Crop Prod 157:112892. https://doi.org/10.1016/j.indcrop.2020.112892
Article CAS Google Scholar
European Food Safety Authority (EFSA). http://www.efsa.europa.eu/. Accessed 25 Jan 2022
Gallaher CM, Gallaher DD, Peterson S (2012) Development and validation of a spectrophotometric method for quantification of total glucosinolates in Cruciferous vegetables. J Agric Food Chem 60:1358–1362. https://doi.org/10.1021/jf2041142
Article CAS PubMed Google Scholar
Gallia MC, Echeverri Del Sarto J, Bongiovanni GA (2021) Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana. J Genet Eng Biotechnol 19(1):181. https://doi.org/10.1186/s43141-021-00280-6
Article PubMed PubMed Central Google Scholar
German Commission D (1989) Monographie BGA/BfArM, Kommission D, 146th ed.
Google Scholar
Giallourou NS, Rowland IR, Rothwell SD, Packham G, Commane DM, Swann JR (2019) Metabolic targets of watercress and PEITC in MCF-7 and MCF-10A cells explain differential sensitisation responses to ionising radiation. Eur J Nutr 58(6):2377–2391. https://doi.org/10.1007/s00394-018-1789-8
Article CAS PubMed Google Scholar
Gilby AC, Wainwright H (1989) Use of tissue culture in improvement of watercress (Rorippa Nasturtium aquaticum L. Hayek). Acta Hortic 244:1
Google Scholar
Głusiuk A (2018) Dbałość o urodę kobiet na podstawie traktatów Metrodory z Bizancjum i Trotuli z Salerno. Semin Dermatol 39(1):145–154. (In Polish). https://doi.org/10.21852/sem.2018.1.12
Article Google Scholar
González-Benito EE, Martín C (2011) In vitro preservation of Spanish biodiversity. In Vitro Cell Dev Biol Plant 47(1):46–54. https://doi.org/10.1007/s11627-010-9333-4
Article Google Scholar
González-Molina E, Domínguez-Perles R, Moreno DA, García-Viguera C (2010) Natural bioactive compounds of Citrus limon for food and health. J Pharm Biomed Anal 51(2):327–345. https://doi.org/10.1016/j.jpba.2009.07.027
Article CAS PubMed Google Scholar
Greene E (1896) Some fundamentals of nomenclature. Science 3(53):13–16
Article CAS PubMed Google Scholar
Grzegorczyk I, Wysokińska H (2008) Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; effects of triacontanol. Acta Soc Bot Pol 77:99–104. https://doi.org/10.5586/asbp.2008.013
Article CAS Google Scholar
Hayta S, Akgun IH, Ganzera M, Bedir E, Gurel A (2011) Shoot proliferation and HPLC-determination of iridoid glycosides in clones of Gentiana cruciata L. Plant Cell Tissue Organ Cult 107(1):175–180. https://doi.org/10.1007/s11240-011-9961-3
Article CAS Google Scholar
Hlatshwayo NA, Amoo SO, Olowoyo JO, Doležal K (2020) Efficient micropropagation protocol for the conservation of the endangered Aloe peglerae, an ornamental and medicinal species. Plan Theory 9(4):1–10. https://doi.org/10.3390/plants9040506
Article CAS Google Scholar
Holst B, Williamson G (2004) A critical review of the bioavailability of glucosinolates and related compounds. Nat Prod Rep 21(3):425–447. https://doi.org/10.1039/b204039p
Article CAS PubMed Google Scholar
Hoseini HF, Gohari AR, Saeidnia S, Majd NS, Hadjiakhoondi A (2009) The effect of Nasturtium officinale on blood glucose level in diabetic rats. Pharmacology Online 3:866–871
Google Scholar
IUCN (2021) The IUCN red list of threatened species. https://www.iucnredlist.org/. Accessed 22 Jan 2022
Javid A, Gampe N, Gelana F, György Z (2021) Enhancing the accumulation of rosavins in Rhodiola rosea l. Plants grown in vitro by precursor feeding. Agronomy 11(12):2531. https://doi.org/10.3390/agronomy11122531
Article CAS Google Scholar
Jeon J, Bong SJ, Park JS, Park YK, Arasu MV, Al-Dhabi NA, Park SU (2017) De novo transcriptome analysis and glucosinolate profiling in watercress (Nasturtium officinale R. Br.). BMC Genomics 18(1):1–14. https://doi.org/10.1186/s12864-017-3792-5
Article CAS Google Scholar
Jesionek A, Kokotkiewicz A, Wlodarska P, Zabiegala B, Bucinski A, Luczkiewicz M (2017) Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds. Plant Cell Tissue Organ Cult 131(1):51–64. https://doi.org/10.1007/s11240-017-1261-0
Article CAS Google Scholar
Jesionek A, Kokotkiewicz A, Krolicka A, Zabiegala B, Luczkiewicz M (2018) Elicitation strategies for the improvement of essential oil content in Rhododendron tomentosum (Ledum palustre) bioreactor-grown microshoots. Ind Crop Prod 123:461–469. https://doi.org/10.1016/j.indcrop.2018.07.013
Article CAS Google Scholar
Jiang W, Chen L, Pan Q, Qiu Y, Shen Y, Fu C (2012) An efficient regeneration system via direct and indirect organogenesis for the medicinal plant Dysosma versipellis (Hance) M. Cheng and its potential as a podophyllotoxin source. Acta Physiol Plant 34(2):631–639. https://doi.org/10.1007/s11738-011-0864-z
Article CAS Google Scholar
Karuppusamy S (2009) A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J Med Plant Res 3(13):1222–1239
CAS Google Scholar
Kikowska M, Thiem B, Szopa A, Ekiert H (2020) Accumulation of valuable secondary metabolites: phenolic acids and flavonoids in different in vitro systems of shoot cultures of the endangered plant species—Eryngium alpinum L. Plant Cell Tissue Organ Cult 141(2):381–391. https://doi.org/10.1007/s11240-020-01795-5
Article CAS Google Scholar
Kikowska M, Chanaj-Kaczmarek J, Derda M, Budzianowska A, Thiem B, Ekiert H, Szopa A (2022) The evaluation of phenolic acids and flavonoids content and antiprotozoal activity of Eryngium species biomass produced by biotechnological methods. Molecules 27(2):363. https://doi.org/10.3390/molecules27020363
Article CAS PubMed PubMed Central Google Scholar
Klimek-Szczykutowicz M, Szopa A, Ekiert H (2018a) Nasturtium officinale (rukiew wodna)—źródło surowców roślinnych o cennych walorach kosmetycznych, leczniczych i prozdrowotnych. Pol J Cosmetol 21(4):290–299. (In Polish)
Google Scholar
Klimek-Szczykutowicz M, Szopa A, Ekiert H (2018b) Chemical composition, traditional and professional use in medicine, application in environmental protection, position in food and cosmetics industries, and biotechnological studies of Nasturtium officinale (watercress)—a review. Fitoterapia 129(April):283–292. https://doi.org/10.1016/j.fitote.2018.05.031
Article CAS PubMed Google Scholar
Klimek-Szczykutowicz M, Szopa A, Dziurka M, Komsta Ł, Tomczyk M, Ekiert H (2020) The influence of Nasturtium officinale R. Br. agar and agitated microshoot culture media on glucosinolate and phenolic acid production, and antioxidant activity. Biomolecules 10(9):1216. https://doi.org/10.3390/biom10091216
Article CAS PubMed PubMed Central Google Scholar
Klimek-Szczykutowicz M, Prokopiuk B, Dziurka K, Pawłowska B, Ekiert H, Szopa A (2021) The influence of different wavelengths of LED light on the production of glucosinolates and phenolic compounds and the antioxidant potential in in vitro cultures of Nasturtium officinale (watercress). Plant Cell Tissue Organ Cult 149(22):113–122. https://doi.org/10.1007/s11240-021-02148-6
Article CAS Google Scholar
Krol A, Kokotkiewicz A, Szopa A, Ekiert H, Luczkiewicz M (2020) Bioreactor-grown shoot cultures for the secondary metabolite production. In Ramawat, K.G., Ekiert, H.M., Goyal, S. Plant cell and tissue differentiation and secondary metabolites; Springer: Cham., ISBN 9783030112530
Google Scholar
Li Q, Zhan M, Chen W, Zhao B, Yang K, Yang J, Yi J, Huang Q, Mohan M, Hou Z, Wang J (2016) Phenylethyl isothiocyanate reverses cisplatin resistance in biliary tract cancer cells via glutathionylation-dependent degradation of Mcl-1. Oncotarget 7(9):10271–10282. https://doi.org/10.18632/oncotarget.7171
Article PubMed PubMed Central Google Scholar
Linnaeli C (1753) Species Plantarum, Exhibentes Plantas Rite Cognitas, Ad Genera Relatas, Cum Differentiis Specipicis, Nominibus Trivialibus, Synonymis Selectis, Locis Natalibus, Secundum Systema Sexuale Digestas. Tomus II
Google Scholar
Lucchesini M, Bertoli A, Mensuali-Sodi A, Cappelli E, Noccioli C, Luciardi L, Pistelli L (2010) Cytisus aeolicus Guss. ex Lindl. in vitro cultures and genistin production. Cent Eur J Biol 5(1):111–120. https://doi.org/10.2478/s11535-009-0067-4
Article CAS Google Scholar
Mahdavi S, Kheyrollahi M, Sheikhloei H, Isazadeh A (2019) Antibacterial and antioxidant activities of Nasturtium officinale essential oil on food borne bacteria. Open Microbiol J 13(1):81–85. https://doi.org/10.2174/1874285801913010081
Article CAS Google Scholar
Manton I (1935) The cytological history of watercress (Nasturtium officinale R. Br). Z Indukt Abstamm Vererbungsl 69(1):132–157. https://doi.org/10.1007/BF01762872
Article Google Scholar
Martínez-Sánchez A, Gil-Izquierdo A, Gil MI, Ferreres F (2008) A comparative study of flavonoid compounds, vitamin C, and antioxidant properties of baby leaf Brassicaceae species. J Agric Food Chem 56(7):2330–2340. https://doi.org/10.1021/jf072975+
Article CAS PubMed Google Scholar
Mishra J, Bhandari H, Singh M, Rawat S, Agnihotri RK, Mishra S, Purohit S (2011) Hairy root culture of Picrorhiza kurroa Royle ex Benth.: a promising approach for the production of picrotin and picrotoxinin. Acta Physiol Plant 33(5):1841–1846. https://doi.org/10.1007/s11738-011-0724-x
Article CAS Google Scholar
Mohammadi J, Motlagh FT, Mohammadi N (2017) The effect of hydroalcoholic extract of watercress on parameters of reproductive and sex hormones on the diabetic rats. J Pharm Sci Res 9(8):1334–1338
CAS Google Scholar
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Article CAS Google Scholar
Nieto-Trujillo A, Cruz-Sosa F, Luria-Pérez R, Gutiérrez-Rebolledo GA, Román-Guerrero A, Burrola-Aguilar C, Zepeda-Gómez C, Estrada-Zúñiga ME (2021) Arnica montana cell culture establishment, and assessment of its cytotoxic, antibacterial, α-amylase inhibitor, and antioxidant in vitro bioactivities. Plan Theory 10(11):2300. https://doi.org/10.3390/plants10112300
Article CAS Google Scholar
Ogita S, Usui ÆM, Shibutani ÆN (2009) A simple shoot multiplication procedure using internode explants, and its application for particle bombardment and Agrobacterium-mediated transformation in watercress. J Plant Res 122:455–463. https://doi.org/10.1007/s10265-009-0226-8
Article CAS PubMed Google Scholar
Özyürek M, Güçlü K, Bektaşoğlu B, Apak R (2007) Spectrophotometric determination of ascorbic acid by the modified CUPRAC method with extractive separation of flavonoids–La(III) complexes. Anal Chim Acta 588:88–95. https://doi.org/10.1016/j.aca.2007.01.078
Article CAS PubMed Google Scholar
Pandey DK, Ayangla NW (2018) Biotechnological aspects of the production of natural sweetener glycyrrhizin from Glycyrrhiza sp. Phytochem Rev 17(2):397–430. https://doi.org/10.1007/s11101-017-9540-2
Article CAS Google Scholar
Petrova M, Zayova E, Vassilevska-Ivanova R, Vlahova M (2012) Biotechnological approaches for cultivation and enhancement of secondary metabolites in Arnica montana L. Acta Physiol Plant 34(5):1597–1606. https://doi.org/10.1007/s11738-012-0987-x
Article CAS Google Scholar
Pietrosiuk A, Furmanowa M (2006) Biotechnologia roślin w ochronie zdrowia człowieka. Biotechnologia 4(75):117–123. (In Polish)
Google Scholar
Priyadharshini S, Kannan N, Manokari M, Shekhawat MS (2020) In vitro regeneration using twin scales for restoration of critically endangered aquatic plant Crinum malabaricum Lekhak & Yadav: a promising source of galanthamine. Plant Cell Tissue Organ Cult 141(3):593–604. https://doi.org/10.1007/s11240-020-01818-1
Article CAS Google Scholar
Qaderi A, Mehrafarin A, Rezazadeh S, Zarinpanjeh N (2021) The efficient method for in vitro micropropagation of Ginkgo biloba L. J Med Plants 20(78):78–89. https://doi.org/10.52547/jmp.20.78.78
Article Google Scholar
Quezada-Lázaro R, Fernández-Zuñiga EA, García A, Garza-González E, Alvarez L, Camacho-Corona MR (2016) Antimycobacterial compounds from Nasturtium officinale. Afr Tradit Complement Altern Med 13(2):31–34
Article Google Scholar
Rameshkumar R, Satish L, Pandian S, Rathinapriya P, Rency AS, Shanmugaraj G, Pandian SK, Leung DWM, Ramesh M (2018) Production of squalene with promising antioxidant properties in callus cultures of Nilgirianthus ciliatus. Ind Crop Prod 126:357–367. https://doi.org/10.1016/j.indcrop.2018.10.031
Article CAS Google Scholar
Rawat JM, Rawat B, Agnihotri RK, Chandra A, Nautiyal S (2013) In vitro propagation, genetic and secondary metabolite analysis of Aconitum violaceum Jacq.: a threatened medicinal herb. Acta Physiol Plant 35(8):2589–2599. https://doi.org/10.1007/s11738-013-1294-x
Article CAS Google Scholar
Rubin E, Aziz ZA, Surugau N (2018a) Glucosinolates content of in vitro grown Nasturtium officinale (watercress). ASM Sci J 11(Special Issue 2):132–139
Google Scholar
Rubin E, Aziz ZA, Surugau N (2018b) Glucosinolates content in non-elicited plant culture, elicited plant culture and wild plant of watercress (Nasturtium officinale). Transact Sci Technol 5(1):40–45
Google Scholar
Sadeghi H, Mostafazadeh M, Sadeghi H, Naderian M, Barmak MJ, Talebianpoor MS, Mehraban F (2014) In vivo anti-inflammatory properties of aerial parts of Nasturtium officinale. Pharm Biol 52(2):169–174. https://doi.org/10.3109/13880209.2013.821138
Article PubMed Google Scholar
Satdive R, Shinde AN, Singh S, Kamble S, Singh S, Malpathak N, Fulzele DP (2015) Aggregate cell suspension cultures of Psoralea corylifolia improved phytoestrogens production. Biotechnol Bioprocess Eng 20(2):373–379. https://doi.org/10.1007/s12257-014-0536-0
Article CAS Google Scholar
Schuchardt JP, Hahn A, Greupner T, Wasserfurth P, Rosales-López M, Hornbacher J, Papenbrock J (2019) Watercress—cultivation methods and health effects. J Appl Bot Food Qual 92:232–239. https://doi.org/10.5073/JABFQ.2019.092.032
Article CAS Google Scholar
Shahani S, Behzadfar F, Jahani D, Ghasemi M, Shaki F (2017) Antioxidant and anti-inflammatory effects of Nasturtium officinale involved in attenuation of gentamicin-induced nephrotoxicity. Toxicol Mech Methods 27(2):107–114. https://doi.org/10.1080/15376516.2016.1258748
Article CAS PubMed Google Scholar
Shehzad MA, Khan MA, Ali A, Mohammad S, Noureldeen A, Darwish H, Ali A, Ahmad A, Khan T, Khan RS (2021) Interactive effects of zinc oxide nanoparticles and different light regimes on growth and silymarin biosynthesis in callus cultures of Silybum marianum L. Artif Cells Nanomed Biotechnol 49(1):523–535. https://doi.org/10.1080/21691401.2021.1946069
Article CAS PubMed Google Scholar
Shinde AN, Malpathak N, Fulzele DP (2009) Induced high frequency shoot regeneration and enhanced isoflavones production in Psoralea corylifolia. Rec Nat Prod 3(1):38–45
CAS Google Scholar
Singh T, Yadav R, Agrawal V (2020) Effective protocol for isolation and marked enhancement of psoralen, daidzein and genistein in the cotyledon callus cultures of Cullen corylifolium (L.) Medik. Ind Crop Prod 143:111905. https://doi.org/10.1016/j.indcrop.2019.111905
Article CAS Google Scholar
Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299:152–178. https://doi.org/10.1016/S0076-6879(99)99017-1
Article CAS Google Scholar
Sowmya M, Jinu U, Sarathikannan D, Geetha N, Girija S, Venkatachalam P (2020) Effect of silver nitrate and growth regulators on direct shoot organogenesis and in vitro flowering from internodal segment explants of Alternanthera sessilis L. Biocatal Agric Biotechnol 30:101855. https://doi.org/10.1016/j.bcab.2020.101855
Article Google Scholar
Steingroewer J, Bley T, Georgiev V, Ivanov I, Lenk F, Marchev A, Pavlov A (2013) Bioprocessing of differentiated plant in vitro systems. Eng Life Sci 13(1):26–38. https://doi.org/10.1002/elsc.201100226
Article CAS Google Scholar
Suroowan S, Mahomoodally MF (2016) A comparative ethnopharmacological analysis of traditional medicine used against respiratory tract diseases in Mauritius. J Ethnopharmacol 177:61–80. https://doi.org/10.1016/j.jep.2015.11.029
Article PubMed Google Scholar
Szewczyk A, Kwiecień I, Grabowski M, Rajek K, Cavò E, Taviano MF, Miceli N (2021) Phenylalanine increases the production of antioxidant phenolic acids in Ginkgo biloba cell cultures. Molecules 26(16):4965. https://doi.org/10.3390/molecules26164965
Article CAS PubMed PubMed Central Google Scholar
Szpitter A, Królicka A (2005) Stymulujący wpływ elicytorów biotycznych na produkcję farmakologicznie czynnych metabolitów wtórnych w roślinnych kulturach in vitro. Biotechnologia 4(71):82–108. (In Polish)
Google Scholar
Teixidor-Toneu I, Martin GJ, Ouhammou A, Puri RK, Hawkins JA (2016) An ethnomedicinal survey of a Tashelhit-speaking community in the High Atlas, Morocco. J Ethnopharmacol 188:96–110. https://doi.org/10.1016/j.jep.2016.05.009
Article PubMed Google Scholar
Thibane VS, Ndhlala AR, Finnie JF, Van Staden J (2019) Modulation of the enzyme activity of secretory phospholipase A₂, lipoxygenase and cyclooxygenase involved in inflammation and disease by extracts from some medicinal plants used for skincare and beauty. S Afr J Bot 120:198–203. https://doi.org/10.1016/j.sajb.2018.06.001
Article Google Scholar
USDA Food Composition Databases (USDA). https://ndb.nal.usda.gov/ndb/
Verpoorte R, Contin A, Memelink J (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1(1):13–25. https://doi.org/10.1023/A:1015871916833
Article CAS Google Scholar
Wainwright H, Marsh J (1986) The micropropagation of watercress (Rorippa nasturtium-aquatic um L.). J Hortic Sci 61(2):251–256. https://doi.org/10.1080/14620316.1986.11515698
Article Google Scholar
Wielanek M, Królicka A, Bergier K, Gajewska E, Skłodowska M (2009) Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production. Biotechnol Lett 31(6):917–921. https://doi.org/10.1007/s10529-009-9953-0
Article CAS PubMed Google Scholar
Winson KWS, Chew BL, Sathasivam K, Subramaniam S (2020) The establishment of callus and cell suspension cultures of Hylocereus costaricensis for the production of betalain pigments with antioxidant potential. Ind Crop Prod 155:112750. https://doi.org/10.1016/j.indcrop.2020.112750
Article CAS Google Scholar
Yancheva S, Georgieva L, Badjakov I, Dincheva I, Georgieva M, Georgiev V, Kondakova V (2019) Application of bioreactor technology in plant propagation and secondary metabolite production. J Cent Eur Agric 20(1):321–340. https://doi.org/10.5513/JCEA01/20.1.2224
Article Google Scholar
Yaricsha CA, Rissyelly R, Katrin K (2017) ACE Inhibitory activity, total phenolic and flavonoid content of watercress (Nasturtium officinale R. Br) extract. Pharmacogn J 9(2):249–251. https://doi.org/10.5530/pj.2017.2.42
Article CAS Google Scholar
Yazdanparast R, Bahramikia S, Ardestani A (2008) Nasturtium officinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic rats. Chem Biol Interact 172(3):176–184. https://doi.org/10.1016/j.cbi.2008.01.006
Article CAS PubMed Google Scholar
Yehuda H, Soroka Y, Zlotkin-Frušić M, Gilhar A, Milner Y, Tamir S (2012) Isothiocyanates inhibit psoriasis-related proinflammatory factors in human skin. Inflamm Res 61(7):735–742. https://doi.org/10.1007/s00011-012-0465-3
Article CAS PubMed Google Scholar
Yuan J-M, Stepanov I, Murphy SE, Wang R, Allen S, Jensen J, Strayer L, Adams-Haduch J, Upadhyaya P, Le C, Kurzer MS, Nelson HH, Yu MC, Hatsukami D, Hecht SS (2016) Clinical trial of 2-phenethyl isothiocyanate as an inhibitor of metabolic activation of a tobacco-specific lung carcinogen in cigarette smokers. Cancer Prev Res 9(5):396–405. https://doi.org/10.1158/1940-6207.CAPR-15-0380.Clinical
Article CAS Google Scholar
Zafar R, Zahoor M, Shah AB, Majid F (2017) Determination of antioxidants and antibacterial activities, total phenolic, polyphenol and pigment contents in Nasturtium officinale. Pharmacology Online 1:11–18
CAS Google Scholar
Zeb A (2015) Phenolic profile and antioxidant potential of wild watercress (Nasturtium officinale L.). Springerplus 4(1):1–7. https://doi.org/10.1186/s40064-015-1514-5
Article Google Scholar

Download references

Author information

Authors and Affiliations

Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, Kraków, Poland
Marta Klimek-Szczykutowicz
Department of Dermatology, Cosmetology and Aesthetic Surgery, Medical College, The Institute of Medical Sciences, Jan Kochanowski University, Kielce, Poland
Marta Klimek-Szczykutowicz
Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University, Medical College, Krakow, Poland
Halina Ekiert & Agnieszka Szopa

Authors

Marta Klimek-Szczykutowicz
View author publications
You can also search for this author in PubMed Google Scholar
Halina Ekiert
View author publications
You can also search for this author in PubMed Google Scholar
Agnieszka Szopa
View author publications
You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Agnieszka Szopa .

Editor information

Editors and Affiliations

Department of Botany, University of Calcutta, Kolkata, West Bengal, India
Sumita Jha
Department of Botany, Barasat Government College, Kolkata, West Bengal, India
Mihir Halder

Ethics declarations

The study was realized as a part of the research projects N/42/DBS/000122 and N42/DBS/000273 supported by the Polish Ministry of Science and Higher Education.

Author Contribution Statement

All the authors read and approved the manuscript in its final form. All authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

Copyright information

About this chapter

Cite this chapter

Klimek-Szczykutowicz, M., Ekiert, H., Szopa, A. (2023). Biotechnological Studies on Nasturtium officinale (Watercress): an Endangered Species of Significant Relevance in Medicine, Cosmetic, and Food Industries. In: Jha, S., Halder, M. (eds) Medicinal Plants: Biodiversity, Biotechnology and Conservation. Sustainable Development and Biodiversity, vol 33. Springer, Singapore. https://doi.org/10.1007/978-981-19-9936-9_22

Download citation

DOI: https://doi.org/10.1007/978-981-19-9936-9_22
Published: 08 July 2023
Publisher Name: Springer, Singapore
Print ISBN: 978-981-19-9935-2
Online ISBN: 978-981-19-9936-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)

Publish with us

Policies and ethics