Abstract
Chlorosis and mottling in red-fleshed pitaya (Selenicereus monacanthus) were observed in Bay, Laguna, Philippines. Cactus virus X (CVX) was detected in symptomatic red-fleshed pitaya stems using a genus- and species-specific reverse transcription-polymerase chain reaction (RT-PCR) assay. The PCR assay confirmed that the virus belongs to the genus Potexvirus and specifically to the CVX group. The identity of the virus was further validated through DNA sequencing, which revealed that the CVX isolate from symptomatic red-fleshed pitaya stems in Philippine (PHCVX01) had 96.98% and 96.59% similarities with the CVX isolate from Taiwan (AF308158) and Korea (LC128411), respectively. This is the first scientific report on the detection of CVX in dragon fruit in the Philippines. Indexing of commercial stem cuttings may be beneficial in controlling the spread of CVX in dragon fruit farms.
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Data is available upon reasonable request from the corresponding author.
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Acknowledgements
The authors would like to thank Fe Dela Cueva, Loida Pascual, Pamela Quintos, Joseph Lagman, Ryan Tiongco, Alben Manilay, Richard Sanchez for the technical assistance.
Funding
We thank the Department of Agriculture- Bureau of Agricultural Research (DA-BAR) for the financial support. In-kind support was received from the Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños and the Department of Science and Technology- Science Education Institute.
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Evallo, E., Taguiam, J.D., Tumolva, J.A. et al. First report of Cactus virus X in Selenicereus monacanthus in the Philippines. Indian Phytopathology 75, 1237–1239 (2022). https://doi.org/10.1007/s42360-022-00555-z
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DOI: https://doi.org/10.1007/s42360-022-00555-z