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Development of microsatellite markers for Adenophora palustris (Campanulaceae), a critically endangered wetland plant species in Japan

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Abstract

Adenophora palustris is an endangered wetland plant species in Japan. The population sizes of this species are extremely small and currently only five extant populations are known. Consequently, A. palustris is classified as “critically endangered” in the Japanese Red Data Book. We isolated and characterized nine microsatellite loci in this species. The number of alleles ranged from 2 to 8, observed heterozygosity from 0.26 to 0.81, and expected heterozygosity from 0.34 to 0.79. The markers described here will be useful for investigating the genetic diversity, genetic structure, and gene flow of A. palustris, and also in planning for the conservation of this species.

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References

  • Goudet J (1995) FSTAT (V. 1.2): a computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • Lian C, Hogetsu T (2002) Development of microsatellite markers in black locust (Robinia pseudoacasia) using a dual-suppression-PCR technique. Mol Ecol Notes 2:211–213

    CAS  Google Scholar 

  • Lian C, Wadud MA, Geng Q, Shimatani K, Hougetsu T (2006) An improved technique for isolating codominant compound microsatellite markers. J Plant Res 119:415–417

    Article  CAS  PubMed  Google Scholar 

  • Milligan B (1992) Plant DNA isolation. In: Hoelzel AR (ed) Molecular genetic analysis of populations: a practical approach. IRL Press, Oxford, pp 59–88

    Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386

    Google Scholar 

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Acknowledgments

This work was supported by the Global Environment Research Fund (D-0903) of the Ministry of the Environment, Japan.

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Authors and Affiliations

  1. Chuden Engineering Consultants CO., LTD., 2-3-30, Deshio, Minami-Ku, Hiroshima, Hiroshima Prefecture, 734-8510, Japan

    Ikuko Masumoto & Kuniaki Otake

  2. Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Shingo Kaneko & Yuji Isagi

Authors
  1. Ikuko Masumoto
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  2. Shingo Kaneko
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  3. Kuniaki Otake
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  4. Yuji Isagi
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Correspondence to Ikuko Masumoto.

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Masumoto, I., Kaneko, S., Otake, K. et al. Development of microsatellite markers for Adenophora palustris (Campanulaceae), a critically endangered wetland plant species in Japan. Conservation Genet Resour 3, 163–165 (2011). https://doi.org/10.1007/s12686-010-9314-2

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  • DOI: https://doi.org/10.1007/s12686-010-9314-2

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