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In vitro Evaluation of Selective Cytotoxic Activity of Chaerophyllum macropodum Boiss. on Cultured Human SH-SY5Y Neuroblastoma Cells

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Abstract

Neuroblastoma is the most common solid tumor in children. New treatment approaches are needed because of the harmful side effects and costs of the methods used in the treatment of neuroblastoma. Medicinal and aromatic plants are important for new treatment approaches due to their minimal side effects and economic advantages. Therefore, the present study was carried out to examine the cytotoxic effect of Chaerophyllum macropodum extract on human neuroblastoma (SH-SY5Y) and fibroblast (HDFa) cell lines. 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase release (LDH) assays were used to determine the cytotoxic effect of C. macropodum. The extracts were analyzed for their phenolic content by HPLC–PDA. Major components were determined as 63.600% o-coumaric acid, 15.606% catechine hydrate, 8.713% rosmarinic acid, 4.376% clorogenic acid, and 3.972% salicylic acid. The obtained results from cytotoxicity testing revealed that C. macropodum exerted a significant cytotoxic effect on human neuroblastoma cells at all tested concentrations (p < 0.05). But it did not lead to any cytotoxic potential on human fibroblasts. As a result, the obtained data clearly revealed C. macropodum exerted a selective cytotoxic action on neuroblastoma cells for the first time.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Letter, Bitlis Eren University, Bitlis, Turkey

    Fatih Çağlar Çelikezen

  2. Department of Medical Biology, Faculty of Medicine, Atatürk University, Erzurum, Turkey

    Hasan Türkez

  3. Department of Biology, Faculty of Education, Van Yuzuncu Yil University, Van, Turkey

    Mehmet Firat

  4. Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey

    Mehmet Enes Arslan & Sena Öner

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  1. Fatih Çağlar Çelikezen
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FÇÇ and HT designed the study, commented on the results, and wrote the manuscript. MF collected plants and made his scientific diagnosis. MEA and SÖ performed the analysis and wrote the manuscript.

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Correspondence to Fatih Çağlar Çelikezen.

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Çelikezen, F.Ç., Türkez, H., Firat, M. et al. In vitro Evaluation of Selective Cytotoxic Activity of Chaerophyllum macropodum Boiss. on Cultured Human SH-SY5Y Neuroblastoma Cells. Neurotox Res 40, 1360–1368 (2022). https://doi.org/10.1007/s12640-022-00537-z

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