Abstract
The aim of this research was to introduce and characterize a novel plant-based polysaccharide. Ornithogalum cuspidatum (OC) was used for mucilage extraction and chemical, physical, thermal and rheological properties of raw extracted mucilage (OMA), purified (OMB) and acid hydrolyzed mucilage (OMC) were compared. The purified mucilage (OMB) had the highest molecular weight (1.7 × 106 kDa). Nuclear magnetic resonance spectroscopy showed that the extracted mucilage is a member of arabinogalactan family. high performance liquid chromatography analysis showed that the mucilage is a hetropolysaccharide composed of galactose, glucose, arabinose and galacturonic acid, that galactose was the main monosaccharide in all samples. The functional groups and morphology of samples were characterized by Fourier transform infrared and Field emission scanning electron microscope analyses, respectively. Zeta potential was negative for three samples indicating the anionic nature of mucilage. The thermal analysis showed that the highest thermal stability was related to sample OMC. Water holding capacity of sample OMB was significantly higher than other two samples (p < 0.05). According to rheological studies, pseudoplastic behavior of three samples was confirmed. Sample OMB had the highest viscosity, and three samples followed the Herschel Balkley model. In the frequency sweep test, the loss modulus of all samples was greater than the storage modulus that indicates a weak gel structure of mucilage. In general, this research revealed that the OC mucilage as a liquid viscoelastic material can be introduced as a new thickener or stabilizer in food industry and purification and partial hydrolysis can affect its physicochemical and functional properties.
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Z. Gao, Y. Fang, Y. Cao, H. Liao, K. Nishinari, G. Phillips, Hydrocolloid-food component interactions. Food Hydrocoll. 68, 149–156 (2016)
M. Kakar, M. Naveed, M. Saeed, S. Zhao, M. Rasheed, S. Firdos, R. Manzoor, Y. Deng, R. Dai, A review on structure, extraction, and biological activities of polysaccharides isolated from Cyclocarya paliurus (Batalin) Iljinskaja. Int. J. Biol. Macromol. 156, 420–429 (2020)
N. Farhadi, Structural elucidation of a water-soluble polysaccharide isolated from Balangu shirazi (Lallemantia royleana) seeds. Food Hydrocoll. 72, 263–270 (2017)
M. Kaur, R. Kaur, S. Punia, Characterization of mucilages extracted from different flaxseed (Linum usitatissiumum L.) cultivars: a heteropolysaccharide with desirable functional and rheological properties. Int. J. Biol. Macromol. 117, 919–927 (2018)
V. Manhivi, S. Venter, A. Amonsou, T. Kudanga, Composition, thermal and rheological properties of polysaccharides from amadumbe (Colocasia esculenta) and cactus (Opuntia spp.). Carbohydr. Polym. 195, 163–169 (2018)
Y. Timilsena, R. Adhikar, S. Kasapi, B. Adhikar, Molecular and functional characteristics of purified gum from Australian chia seeds. Carbohydr. Polym. 136, 128–136 (2015)
F.F. Tosin, R. Barraza, J. Petkowicz Silveira, E. Sassaki Santos, P.A.J. Gorin, M. Iacomini, Rheological and structural characteristics of peach tree gum exudate. Food Hydrocoll. 24, 486–493 (2010)
M. El-Aziz, H. Haggag, M. Kaluoubi, L. Hassan, M. El-Sayed, A. Sayed, Characterization of ethanol precipitated cress seed and flaxseed mucilages compared with commercial guar gum. Am. J. Food Technol. 3, 84–91 (2016)
R. Vignesh, B. Nair, Extraction and characterization of mucilage from the leaves of Hibiscus Rosa-sinensisi linn. (Malvaceae). Int. J. Pharm. Sci. Res. 7, 2883–2890 (2018)
A. Muyideen, Comparative study on natural gum used in treatment of fecal impaction. J. Med. Care Res. Rev. 1, 44–49 (2018)
E. Vadivel, S. Naik, Isolation and characterization of natural mucillage from Cucumis melo L. Int. J. Pharm. Res. 8, 33–37 (2016)
A. Amini, S. Razavi, Dilute solution properties of Balangu (Lallemantia royleana) seed gum: effect of temperature, salt, and sugar. Int. J. Biol. Macromol. 51, 235–243 (2012)
F. Cuomo, S. Iacovino, G. Cinelli, M.C. Messia, E. Marconi, F. Lopez, Effect of additives on chia mucilage suspensions: a rheological approach. Food Hydrocoll. 109, 106118 (2020)
A.K.F.I. Câmara, P.K. Okuro, R.L. da Cunha, A.M. Herrero, C. Ruiz-Capillas, M.A.R. Pollonio, Chia (Salvia hispanica L.) mucilage as a new fat substitute in emulsified meat products: technological, physicochemical, and rheological characterization. LWT Food Sci. Technol. 125, 109193 (2020)
M.E. Morales-Tovar, E.G. Ramos-Ramírez, J.A. Salazar-Montoya, Modeling and optimization of the parameters affecting extraction of the chan seed mucilage (Hyptis suaveolens (L.) Poit) by mechanical agitation (MA) and ultrasound-assisted extraction (UAE) in a multiple variables system. Food Bioprod. Process. 120, 166–178 (2020)
G.S. Souza, R.C. Bergamasco, A.P. Stafussa, G.S. Madrona, Ultrasound-assisted extraction of Psyllium mucilage: evaluation of functional and technological properties. Emirates J. Food Agri. 32(4), 238–244 (2020)
D. Fortuna, S.S. Mardjan, T.C. Sunarti, E. Darmawati, S.M. Widayati, N. Purwanti, Extraction and characteristic of Dioscorea alata mucilage. IOP Conf. Ser.: Earth Environ. Sci. 542, 012016 (2020)
E. Nazifi, A. Delazar, A. Movafeghi, S. Hemmati, H. Nazemiyeh, L. Nahar, S. Sarkar, GC-MS analysis of the dichloromethane extract of the bulbs of Ornithogalum cuspidatum Bert. (Family: Liliaceae) from Iran. Rec. Nat. Prod. 3, 94–99 (2008)
H. Asadi, M. Orangi, D. Shanehbandi, Z. Babaloo, A. Delazar, L. Mohammadnejad, F. Shahneh, S. Valiyari, B. Baradaran, Methanolic fractions of Ornithogalum cuspidatum induce apoptosis in PC-3 prostate cance cell line and WEHI-164 fibrosarcoma cancer cell line. Adv. Pharm. Bull. 4, 455–458 (2014)
D. Dastan, A. Aliahmadi, Antioxidant and antibacterial studies on different extracts of Ornithogalum cuspidatum Bertol from Iran. Biol. Forum Int. J. 7, 1072–1075 (2015)
A. Delazar, E. Nazifi, A. Movafeghi, H. Nazemiyeh, S. Hemmati, L. Nahar, S. Sarker, Analyses of phytosterols and free radical scavengers in the bulbs of Ornithogalum cuspidatum Bertol. Bol. Latinoam. Caribe Plant. Med. Aromát. 9, 87–92 (2010)
G. Randa, G. Tosun, N. Yayl, SPME GC/MS analysis of three Ornithogalum L. species from Turkey. Rec. Nat. Prod. 4, 497–502 (2015)
R. Chen, F. Meng, Z. Liu, R. Chen, M. Zhang, Antitumor activities of different fractions of polysaccharide purified from Ornithogalum caudatum Ait. Carbohydr. Polym. 80, 845–851 (2010)
A. Guarda, M. Rosell, C. Benedito, M. Galotto, Different hydrocolloids as bread improvers and antisalting agents. Food Hydrocoll. 18, 241–247 (2004)
AOAC, Association of Official Analytical Chemists, Official Methods of Analysis, vol 534, 18th edn. (AOAC International, Washington, DC, 2006)
M. Khalaj, S. Abbasi, Solubilisation of Persian gum: chemical modification using acrylamide. Int. J. Biol. Macromol. 101, 187–195 (2017)
R. Seyfi, M. Kasaai, M. Chaichi, Isolation and structural characterization of a polysaccharide derived from a local gum: Zedo (Amygdalus scoparia Spach). Food Hydrocoll. 87, 915–924 (2018)
P. Kaushik, K. Dowling, R. Adhikari, C. Barrow, B. Adhikari, Effect of extraction temperature on composition, structure and functional properties of flaxseed gum. Food Chem. 215, 333–340 (2017)
M. Cerqueira, B. Souza, J. Simões, J. Teixeira, M. Domingues, M. Coimbra, A. Vicente, Structural and thermal characterization of galactomannans from non-conventional sources. Carbohydr. Polym. 83, 179–185 (2011)
J. Gomes, S. Rocha, M. Pereira, L. Peres, S. Moreno, J. Herrera, M. Coelho, Lipid/particle assemblies based on maltodextrin–gum arabic core as bio-carriers. Colloids Surf. B: Biointerfaces 76, 449–455 (2010)
A. Martin, R. Freitas, G. Sassaki, P. Evangelista, M. Sierakowski, Chemical structure and physical-chemical properties of mucilage from the leaves of Pereskia aculeate. Food Hydrocoll. 70, 20–28 (2017)
D. Betancur-Ancona, J. Lo´pez-Luna, L. Chel-Guerrero, Comparison of the chemical composition and functional properties of Phaseolus lunatus prime and tailing starches. Food Chem. 82, 217–225 (2003)
A. Ghadetaj, H. Almasi, L. Mehryar, Development and characterization of whey protein isolate active films containing nanoemulsions of Grammosciadium ptrocarpum Bioss. essential oil. Food Packag. Shelf Life 16, 31–40 (2018)
L. Piazza, S. Bertini, J. Milany, Extraction and structural characterization of the polysaccharide fraction of Launaea acanthodes gum. Carbohydr. Polym. 79, 449–454 (2010)
S. Razavi, H. Taheri, L. Quinchia, Steady shear flow properties of wild sage (Salvia macrosiphon) seed gum as a function of concentration and temperature. Food Hydrocoll. 25, 451–458 (2011)
W. Cui, G. Mazza, Physicochemical characteristics of flaxseed gum. Food Res. Int. 29, 397–402 (1996)
K. Jahanbin, S. Moini, A.R. Gohari, Z. Emam-Djomeh, P. Masi, Isolation, purification and characterization of a new gum from Acanthophyllum bracteatum roots. Food Hydrocoll. 27, 14–21 (2012)
M. Fathi, M. Mohebbi, A. Koocheki, Introducing Prunus cerasus gum exudates: chemical structure, molecular weight, and rheological properties. Food Hydrocoll. 61, 946–955 (2016)
L. Sciarini, F. Maldonado, P.D. Ribotta, G.T. Pe´rez, A.E. Leo´n, Chemical composition and functional properties of Gleditsia triacanthos gum. Food Hydrocoll. 23, 306–313 (2009)
S.M.A. Razavi, S. Cui, Q. Guo, H. Ding, Some physicochemical properties of sage (Salvia macrosiphon) seed gum. Food Hydrocoll. 35, 453–462 (2013)
M.A. Hesarinejad, S.M.A. Razavi, A. Koocheki, Alyssum homolocarpum seed gum: dilute solution and some physicochemical properties. Int. J. Biol. Macromol. 81, 418–426 (2015)
Y. Viturawong, P. Achayuthakan, M. Suphantharika, Gelatinization and rheological properties of rice starch/xanthan mixtures: effects of molecular weight of xanthan and different salts. Food Chem. 111, 106–114 (2008)
H.A. Khouryieh, T.J. Herald, F. Aramouni, S. Alav, Influence of mixing temperature on xanthan conformation and interaction of xanthan–guar gum in dilute aqueous solutions. Food Res. Int. 39, 964–973 (2006)
G. Sworn, E. Kasapis, Effect of conformation and molecular weight of co-solute on the mechanical properties of gellan gum gels. Food Hydrocoll. 12, 283–290 (1998)
K. Jahanbin, A. Gohari, S. Moini, Z. Emam-Djome, P. Masi, Isolation, structural characterization and antioxidant activity of a new water-soluble polysaccharide from Acanthophyllum bracteatum roots. Int. J. Biol. Macromol. 49, 567–572 (2011)
C. Cao, Y. Li, C. Wang, N. Zhang, X. Zhu, R. Wu, J. Wu, Purification, characterization and antitumor activity of an exopolysaccharide produced by Bacillus velezensis SN-1. Int. J. Biol. Macromol. 156, 54–361 (2020)
L. Andrade, C. Nunes, J. Pereira, Relationship between the chemical components of taro rhizome mucilage and its emulsifying property. Food Chem. 178, 331–338 (2015)
J. Kang, S. Cui, J. Chen, G. Phillips, Y. Wu, Q. Wang, New studies on gum ghatti (Anogeissus latifolia) part I. Fractionation, chemical and physical characterization of the gum. Food Hydrocoll. 25, 1984–1990 (2011)
B. Alizadeh Behbahani, F. Tabatabaei, F. Shahidi, M.A. Hesarinejad, S.A. Mortazavi, M. Mohebbi, Plantago major seed mucilage: optimization of extraction and some physicochemical and rheological aspects. Carbohydr. Polym. 155, 68–77 (2017)
M. Jouki, S.A. Mortazavi, F. Tabatabaei Yazdi, A. Koocheki, Optimization of extraction, antioxidant activity and functional properties of quince seed mucilage by RSM. Int. J. Biol. Macromol. 66, 113–124 (2014)
M.J. Zohuriaan, F. Shokrolahi, Thermal studies on natural and modified gums. Polym. Test. 23, 575–579 (2004)
C. Vendruscolo, C. Ferrero, E. Pineda, J. Silveira, R. Freitas, M. Jiménez-Castellanos, T. Bresolin, Physicochemical and mechanical characterization of galactomannan from Mimosa scabrella: effect of drying method. Carbohydr. Polym. 76, 86–93 (2009)
F. Ma, D. Wang, Y. Zhang, Y. Li, M.W. Qing, C. Tikkanen-Kaukanen, X. Liu, A. Bell, Characterisation of the mucilage polysaccharides from Dioscorea opposite Thunb. with enzymatic hydrolysis. Food Chem. 245, 13–21 (2018)
S.H. Keshani Dokht, Z. Emam Djomeh, M.S. Yarmand, M. Fathi, Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage. Int. J. Biol. Macromol. 118, 485–493 (2018)
S. Bhattacharjee, DLS and zeta potential—What they are and what they are not? J. Controlled Release 235, 337–351 (2016)
F. Ma, Y. Zhang, Y. Yao, Y. Wen, W. Hu, J. Zhang, X. Liu, A. Bell, C. Tikkanen-Kaukanen, Chemical components and emulsification properties of mucilage from Dioscorea opposita Thunb. Food Chem. 228, 315 (2017)
J. Huang, L. Zhou, Peach gum polysaccharide polyelectrolyte: preparation, propertiesand application in layer-by-layer self-assembly. Carbohydr. Polym. 113, 373–379 (2014)
R. Thanatcha, A. Pranee, Extraction and characterization of mucilage in Ziziphus mauritiana Lam. Food Res. Int. 18, 201–212 (2011)
A. Nussinovitch, Plant Gum Exudates of the World: Sources, Distribution, Properties, and Applications (CRC Press/Taylor & Francis Group, 2009), pp. 4–5
C. Sun, S. Gunasekaran, Effects of protein concentration and oil-phase volume fraction on the stability and rheology of menhaden oil-in-water emulsions stabilized by whey protein isolate with xanthan gum. Food Hydrocoll. 23, 165–174 (2009)
G. Sébastien, B. Christoph, A. Mario, L. Pascal, P. Michel, R. Aurore, Impact of purification and fractionation process on the chemical structure and physical properties of locust bean gum. Carbohydr. Polym. 108, 159–168 (2014)
S.H. Hosseini-Parvar, L. Matia-Merino, K. Goh, S.M.A. Razavi, S.A. Mortazav, Steady shear flow behavior of gum extracted from Ocimum basilicum L. seed: effect of concentration and temperature. J. Food Eng. 101, 236–243 (2010)
K. Song, Y. Kim, G. Chan, Rheology of concentrated xanthan gum solutions: steady shear flow behavior. Fibers Polym. 7, 129–138 (2006)
A. Yemenicioğlu, S. Farris, M. Turkyilmaz, S. Gulec, A review of current and future food applications of natural hydrocolloids. Int. J. Food Sci. Technol. 55(4), 1389–1406 (2020)
S. Naji, S.M.A. Razavi, H. Karazhiyan, Effect of thermal treatments on functional properties of cress seed (Lepidium sativum) and xanthan gums: a comparative study. Food Hydrocoll. 28, 75–81 (2012)
J.F. Steffe, Rheological Methods in Food Process Engineering, 2nd edn. (Freeman Press, East Lansing, MI, 1996)
E. Erçelebi, E. Ibanoflu, Rheological properties of whey protein isolate stabilized emulsions with pectin and guar gum. Eur. Food Res. Technol. 229, 281–286 (2009)
L.G. Mandala, T.P. Savvas, A.E. Kostaropoulos, Xanthan and locust bean gum influence on the rheology and structure of a white model-sauce. J. Food Eng. 64, 335–342 (2004)
B. Ghanbarzadeh, Principles of Food and Food Biopolymer Rheology (University of Tehran Press, Tehran, 2009), pp. 37–75
P.A. Williams, G.O. Phillips, Handbook of Hydrocolloid, 2nd edn. (CRC Press, Cambridge, 2009), pp. 1–50
D. Everett, R. McLeod, Interactions of polysaccharide stabilizers with casein aggregates in stirred skim-milk yoghurt. Int. Dairy J. 15, 1175–1183 (2005)
N.G. Diftis, C.G. Biliaderis, V.D. Kiosseoglou, Rheological properties and stability of model salad dressing emulsions prepared with a dry-heated soybean protein isolate–dextran mixture. Food Hydrocoll. 19, 1025–1031 (2005)
A. Taherian, P. Fustier, H. Ramaswamy, Effect of added oil and modified starch on rheological properties, droplet size distribution, opacity and stability of beverage cloud emulsions. J. Food Eng. 77, 687–696 (2006)
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Gheybi, N., Pirouzifard, M.K. & Almasi, H. Ornithogalum cuspidatum mucilage as a new source of plant-based polysaccharide: Physicochemical and rheological characterization. Food Measure 15, 2184–2201 (2021). https://doi.org/10.1007/s11694-021-00814-z
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DOI: https://doi.org/10.1007/s11694-021-00814-z