Abstract
For the first time, suspension cultures produced tropane alkaloids and were obtained from root explants of Hyoscyamus muticus L. It was shown that to obtain a well-proliferating suspension culture from the plant under investigation, a root explant was used. It is advisable to add 6-benzyladenine (0.5 mg L-1) in addition to α-naphthaleneacetic acid (1.0 mg L-1) to the nutrient Murashige and Skoog medium. Histological studies have shown that suspension cultures of H. muticus L. have a complex structure and are characterized by the presence of nodular structures, which are apparently necessary for synthesis and the accumulation of tropane alkaloids. The use of methyl jasmonate (100 and 200 μМ) made it possible to increase the productivity of the suspension culture of H. muticus L., which forms atropine four times more than in plants and more than 5.5 times the root.
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31 May 2022
A Correction to this paper has been published: https://doi.org/10.1007/s11627-022-10273-w
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This paper has been supported by Kazan Federal University Strategic Academic Leadership Program (PRIORITY-2030) and the histological study of suspension culture was performed in Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS with financial support from the government assignment (N: AAAA-A18-11822790083-9) for the FRC Kazan Scientific Center of RAS.
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W. M. A. Performed the experiments, cytological studies, writing – original draft, K. Y. Cytological studies, K. L. Z. data curation, A. G. D. HPLC analysis A. G. R., execution of experiments and statistical analysis and corresponding, T. O. A. supervision.
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The original version of this article was revised: The name of coauthor Kostyukova Yulia Anatolievna was presented incorrectly in the author line of this article as originally published.
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Abdelazeez, W.M.A., Anatolievna, K.Y., Zavdetovna, K.L. et al. Enhanced productivity of atropine in cell suspension culture of Hyoscyamus muticus L.. In Vitro Cell.Dev.Biol.-Plant 58, 593–605 (2022). https://doi.org/10.1007/s11627-022-10262-z
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DOI: https://doi.org/10.1007/s11627-022-10262-z