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Investigation of antimicrobial activities and molecular characterization of the species belong to Origanum, Thymus and Thymbra genera by ISSR

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Abstract

Background:

The aim of this study is to investigate the antimicrobial activities of the species belonging to the genera Origanum L., Thymus L., and Thymbra L. in the Lamiaceae family and molecular characterization using ISSR markers and to determine the correlations between anti-microbial activities of the plant extracts and ISSR loci.

Methods and Results

Anti-microbial active extracts were obtained after 24-hours extraction using either of the three different solvents (ethanol, hexane, and chloroform) from the plants using the Soxhlet device. The effects of extracts on the bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis) were determined using the disc-diffusion method. The species Thymbra spicata var. spicata L., Thymus vulgaris L., Thymus citriodorus, Thymus cilicicus, Origanum syriacum L., and Origanum vulgare L. subsp. hirtum displayed significant anti-microbial activities, while the Origanum minutiflorum, Origanum onites L., Origanum saccatum and Origanum vulgare L. ssp. gracile displayed less activities on the bacterial strains. The plant species under study had a high level of genetic diversity. Significant correlations were determined between the anti-microbial activities of the plant species and the ISSR loci.

Conclusion

Staphylococcus aureus was the most sensitive and Pseudomonas aeruginosa was the least sensitive strain. The ethanol and chloroform extracts were the most effective solvents. ISSR markers were successful for determining high levels of genetic diversity and clustering the species belonging to the genera Origanum, Thymus, and Thymbra. Conducting molecular marker analyses facilitated in distinguishing the species correctly for molecular breeding studies. The studies identified the antimicrobial activities of the plants against the bacteria used in the study and suggested their potential role in the pharmaceutical industry.

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Data Availability

The datasets analyzed during the present study are available from the corresponding author on reasonable request.

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Funding

This project (number FEF19002.19.001) is Sibel Kerem’s Master of Science thesis and was supported by Scientific Research Projects Unit (BAP) of Hitit University.

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Authors and Affiliations

  1. Faculty of Science and Arts, Department of Biology, Hitit University, Çorum, Turkey

    Sibel Kerem & Özlem Özbek

  2. Faculty of Science and Arts, Department of Molecular Biology and Genetics, Hitit University, Çorum, Turkey

    Fetullah Tekin

  3. Republic of Turkey, Ministry of Agriculture and Forestry GAP International Agricultural Research and Training Center, Diyarbakır, Turkey

    Fetullah Tekin

  4. Faculty of Medicine, Department of Microbiology, Hitit University, Çorum, Turkey

    Nezahat Koşar & Ayşe Semra Güreser

Authors
  1. Sibel Kerem
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  2. Nezahat Koşar
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  3. Fetullah Tekin
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  4. Ayşe Semra Güreser
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  5. Özlem Özbek
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Contributions

ÖÖ and ASG designed the study. FT provided the plant samples, SK and NK performed the experiments and collected the data. ÖÖ wrote the manuscript and all the authors confirmed the final draft.

Corresponding author

Correspondence to Özlem Özbek.

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The authors declare that they have no conflict of interest.

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The study was not involving Human Participants and/or Animals.

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Kerem, S., Koşar, N., Tekin, F. et al. Investigation of antimicrobial activities and molecular characterization of the species belong to Origanum, Thymus and Thymbra genera by ISSR. Mol Biol Rep 50, 289–298 (2023). https://doi.org/10.1007/s11033-022-07923-y

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  • DOI: https://doi.org/10.1007/s11033-022-07923-y

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