Abstract
Genetic diversity is an important resource to improve new wheat cultivars in the breeding program. In this study, fluorescence in situ hybridization (FISH) and genotyping by sequencing (GBS) were used to evaluate the genetic diversity and population structure of the Timopheevii group (AtAtGG, 2n = 4x = 28) including 15 Triticum timopheevii Zhuk. accessions and 35 Triticum araraticum Jakubz. accessions. FISH analysis showed that there were 14 and 19 FISH signal variations in the At and G genome of T. araraticum, respectively. But there were only two signal variations in the G genome of T. timopheevii. In this study, 190,402 SNP markers were obtained from GBS, in which the lowest and highest frequencies of SNPs were found in the G and At genomes, respectively. Genetic diversity analysis of the 50 accessions indicated that the mean GD and PIC were 0.30 and 0.26, with the ranges of 0.1–0.5 and 0.1–0.4, respectively. The lowest and highest numbers of SNPs identified on the chromosomes 4G and 2At were 7748 and 17,527, respectively. Structure and cluster analyses divided 50 accessions into two subpopulations (POP1 and POP2). POP1 consisted of most T. araraticum accessions, and POP2 comprised all accessions of T. timopheevii and one T. araraticum accession AS273. In addition, the genetic variance showed that genetic variation was greater within populations (97%) than between populations (3%). The present results provided important information for the improvement of cultivated durum and hexaploid wheat production in future breeding programs of China and other countries.
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The data that support the findings of this study are available in ‘figshare’ with the identifier data DOIs, including two datasets (Tetraploid wheat-GBS.vcf, https://doi.org/10.6084/m9.figshare.16692490.v1 and https://doi.org/10.6084/m9.figshare.17032322.v1).
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This work was supported by the Key Research and Development Program of Sichuan Province, China (2021YFYZ0002) and the National Natural Science Foundation of China (91935303 and 31671682).
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TP and LQZ designed the research and wrote the manuscript. TP, XMJ, DHW, MHZ and XL performed the experiments. MH and XC helped with data analysis, and WL, BJ, LH, SZN, ZWY, DCL, BHW, ZHY and XJC supervised the study. All authors read and approved the final manuscript.
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Peng, T., Jiang, X., Wang, D. et al. Population structure and genetic diversity of Triticum araraticum Jakubz. and Triticum timopheevii Zhuk.. Genet Resour Crop Evol 70, 1799–1815 (2023). https://doi.org/10.1007/s10722-023-01537-4
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DOI: https://doi.org/10.1007/s10722-023-01537-4