Skip to main content
SpringerLink
Log in

Possible intergeneric hybridization between Cancrinia maximowiczii C. Winkl. and Chrysanthemum naktongense (Nakai) Tzvel. × C. ×morifolium Ramat. ‘Aifen’

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Two possible intergeneric putative hybrids between Cancrinia maximowiczii and an interspecific hybrid (Chrysanthemum naktongense × Chrysanthemum ×morifolium ‘Aifen’) were produced without emasculation. The putative hybrids could be identified by ITS sequences and morphological characteristics. The distinct trait of the putative hybrids presented itself by 7–13 white tubular florets bursting out in the outer capitula. Results of flow cytometry analysis and chromosome counting did not support the nature of the putative hybrids. It was found that the ploidy levels of the putative hybrids and parents are diploid, hexaploid and diploid respectively. Whether the paternal parent is a donor of the genome or not, need to be analyzed further by GISH. We propose that C. maximowiczii may be transferred to the subtribe Artemisiinae based on ITS phylogenetic analysis, although the position of the species and the genus Cancrinia could not be confirmed here.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abd El-Twab MH, Kondo K (2001a) Molecular cytogenetic identification of the parental genomes in the intergeneric hybrid between Leucanthemella linearis and Nipponanthemum nipponicum during meiosis and mitosis. Caryologia 54:109–114

    Article  Google Scholar 

  • Abd El-Twab MH, Kondo K (2001b) Genome territories of Dendranthema horaimontana in mitotic nuclei of F1 hybrid between Dendranthema horaimontana and Tanacetum parthenium. Chromosome Sci 5:63–71

    Google Scholar 

  • Abd El-Twab MH, Kondo K (2006) Fluorescence in situ hybridization and genomic in situ hybridization to identify the parental genomes in the intergeneric hybrid between Chrysanthemum japonicum and Nipponanthemum nipponicum. Chromosome Bot 1:7–11

    Article  Google Scholar 

  • Abd El-Twab MH, Kondo K (2007a) FISH physical mapping of 5s rDNA and telomere sequence repeats identified a peculiar chromosome mapping and mutation in Leuchanthemella linearis and Nipponanthemum nipponicum in Chrysanthemum sensu lato. Chromosom Bot 2:11–17

    Article  Google Scholar 

  • Abd El-Twab MH, Kondo K (2007b) Identification of parental chromosomes, intra-chromosomal changes and relationship of the artificial intergeneric hybrid between Chrysanthemum horaimontanum and Tanacetum vulgare by single color and simultaneous bicolor of FISH and GISH. Chromosom Bot 2:113–119

    Article  Google Scholar 

  • Bremer K, Humphries CJ (1993) Generic monograph of the Asteraceae-Anthemideae. Bull Nat Hist Mus Lond Bot 23:71–177

    Google Scholar 

  • Chen JY, Wang CY, Zhao HE, Zhou J (2012) The origin of garden chrysanthemum. Anhui Science and Technology Press, Hefei

    Google Scholar 

  • Deng YM, Chen SM, Lu AM, Chen FD, Tang FP, Guan ZY, Teng NJ (2010a) Production and characterisation of the intergeneric hybrids between Dendranthema morifolium and Artemisia vulgaris exhibiting enhanced resistance to chrysanthemum aphid (Macrosiphoniella sanbourni). Planta 231:693–703

    Article  CAS  PubMed  Google Scholar 

  • Deng YM, Teng NJ, Chen SM, Chen FD, Guan ZY, Song AP, Chang QS (2010b) Reproductive barriers in the intergeneric hybridization between Chrysanthemum grandiflorum (Ramat.) Kitam. and Ajania przewalskii Poljak. (Asteraceae). Euphytica 174:41–50

    Article  Google Scholar 

  • Deng YM, Chen SM, Chen FD, Cheng X, Zhang F (2011) The embryo rescue derived intergeneric hybrid between chrysanthemum and Ajania przewalskii shows enhanced cold tolerance. Plant Cell Rep 30:2177–2186

    Article  CAS  PubMed  Google Scholar 

  • Deng YM, Jiang JF, Chen SM, Teng NJ, Song A, Guan ZY, Fang WM, Chen FD (2012) Combination of multiple resistance traits from wild relative species in Chrysanthemum via trigeneric hybridization. PLoS One 7(8):e44337. doi:10.1371/journal.pone.0044337

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Filatova NS (2007) Plants of Central Asia: plant collections from China and Mongolia. In: Grubov VI (ed) Volume 14a Compositae (Anthemideae). Science Publishers, USA

    Google Scholar 

  • Francisco-Ortega J, Santos-Guerra A, Hines A, Jansen R (1997) Molecular evidence for a Mediterranean origin of the Macaronesian endemic genus Argyranthemum (Asteraceae). Am J Bot 84:1595–1613

    Article  CAS  PubMed  Google Scholar 

  • Gemeinholzer B, Oberprieler C, Bachmann K (2006) Screening the applicability of molecular markers for plant identification using the ITS 1 sequences of Asteraceae species belonging to the tribes Lactuceae and Anthemideae. Unpublished. http://www.ncbi.nlm.nih.gov/nuccore/AJ880341

  • Guo X, Luo C, Wu Z, Zhang X, Cheng X, Huang C (2012) Polyploidy levels of Chinese large-flower chrysanthemum determined by flow cytometry. Afr J Biotechnol 11(31):7789–7794

    Google Scholar 

  • Jeffrey C (2001) Compositae. In: Hanelt P, Institute of Plant Genetics and Crop Plant Research (eds) Mansfeld’s encyclopedia of agricultural and horticultural crops. Springer, Berlin

    Google Scholar 

  • Kondo K, Abd El-Twab MH, Tanaka R (1999) Fluorescence in situ hybridization identifies reciprocal translocation of somatic chromosomes and origin of extra chromosome by an artificial, intergeneric hybrid between Chrysanthemum japonica × Tanacetum vulgare. Chromosom Sci 3:15–19

    Google Scholar 

  • Kondo K, Abd El-Twab MH, Idesawa R, Kimura S, Tanaka R (2003) Genome phylogenetics in Chrysanthemum sensu lato. In: Sharma AK, Sharma A (eds) Plant genome biodiversity and evolution, vol 1, Part A. CRC Press, pp 117–200

  • Li HJ (1993) Chrysanthemums in China. Jiangsu Scientific and Technical Press, Nanjing

    Google Scholar 

  • Li J, Wan Q, Abbott R, Rao G (2013) Geographical distribution of cytotypes in the Chrysanthemum indicum complex as evidenced by ploidy level and genome-size variation. J Syst Evol 51(2):196–204

    Article  Google Scholar 

  • Liesebach H, Naujoks G, Ewald D (2011) Successful hybridisation of normally incompatible hybrid aspen (Populus tremula × P. tremuloides) and eastern cottonwood (P. deltoides). Sex Plant Reprod 24:189–198

    Article  PubMed  Google Scholar 

  • Mitsuoka S, Ehrendorfer F (1972) Cytogenetics and evolution of Matricaria and related genera (Asteraeae-Anthemideae). Österr Bot Z 120:155–200

    Article  Google Scholar 

  • Oberprieler C (2002) A phylogenetic analysis of Chamaemelum Mill. (Compositae: anthemideae) and related genera based upon nrDNA ITS and cpDNA trnL/trnF IGS sequence variation. Bot J Linn Soc 138:255–273

    Article  Google Scholar 

  • Oberprieler C (2004a) On the taxonomic status and the phylogenetic relationships of some unispecific Mediterranean genera of Compositae-Anthemideae I. Brocchia, Endopappus and Heliocauta. Willdenowia 34:39–57

    Article  Google Scholar 

  • Oberprieler C (2004b) On the taxonomic status and the phylogenetic relationships of some unispecific Mediterranean genera of Compositae-Anthemideae II. Daveaua, Leucocyclus and Nananthea. Willdenowia 34:341–350

    Article  Google Scholar 

  • Oberprieler C (2005) Temporal and spatial diversification of circum-Mediterranean Compositae-Anthemideae. Taxon 54:951–966

    Article  Google Scholar 

  • Oberprieler C, Vogt R (2000) The position of Castrilanthemum Vogt & Oberprieler and the phylogeny of Mediterranean Anthemideae (Compositae) as inferred from nrDNA ITS and cpDNA trnL/trnF IGS sequence variation. Plant Syst Evol 225:145–170

    Article  CAS  Google Scholar 

  • Oberprieler C, Vogt R, Watson LE (2006) XVI. Tribe Anthemideae Cass (‘2007’). In: Kadereit JW, Jeffrey C (eds) The families and genera of vascular plants. Flowering plants, eudicots, asterales, vol VIII. Springer Verlag, pp 342–374

  • Oberprieler C, Himmelreich S, Vogt R (2007) A new subtribal classification of the tribe Anthemideae (Compositae). Willdenowia 37:89–114

    Article  Google Scholar 

  • Oberprieler C, Himmelreich S, Kallersjo M, Valles J, Vogt R (2009) Anthemideae. In: Funk V, Susanna A, Steussy TF, Bayer R (eds) Systematics, evolution, and biogeography of the Compositae. IAPT, Vienna, pp 631–666

    Google Scholar 

  • Ronald WG (1982) Intersectional hybridization of Populus sections, Leuce-Aigeiros and Leuce-Tacamahaca. Silv Genet 31:94–99

    Google Scholar 

  • Sanz M, Vilatersana R, Hidalgo O, Garcia-Jacas N, Susanna A, Schneeweiss GM, Vallès J (2008) Molecular phylogeny and evolution of floral characters of Artemisia and allies (Anthemideae, Asteraceae): evidence from nrDNA ETS and ITS sequences. Taxon 57:66–78

  • Shi Z, Chen Y, Chen Y, Lin Y, Liu S, Ge X, Gao T, Zhu S, Liu Y, Yang Q, Humphries CJ, Raab-Straube E, Von Gilbert MG, Nordenstam B, Kilian N, Brouillet L, Illarionova ID, Hind DJN, Jeffrey C, Bayer RJ, Kirschner J, Greuter W, Anderberg AA, Semple JC, Štěpánek J, Freire SE, Martins L, Koyama H, Kawahara T, Vincent L, Sukhorukov AP, Mavrodiev EV, Günter Gottschlich G (2011) Asteraceae (Compositae) [family introduction, glossary, systematic list, and key to tribes]. In: Wu ZY et al (eds) Flora of China, vol 20–21 (Asteraceae). Science Press, Beijing & Missouri Botanical Garden Press, St. Louis

  • Sonboli A, Oberprieler C (2010) Phylogenetic relationship and taxonomic position of Xylanthemum tianschanicum (Krasch.) Muradyan (Compositae, Anthemideae) as inferred from nrDNA ITS data. Biochem Syst Ecol 38:702–707

    Article  CAS  Google Scholar 

  • Sonboli A, Oberprieler C (2012) Insights into the phylogenetic and taxonomic position of Tanacetum semenovii Herder (Compositae, Anthemideae) based on nrDNA ITS sequences data. Biochem Syst Ecol 45:166–170

    Article  CAS  Google Scholar 

  • Tanaka R, Shimotomai N (1961) Cytogenetic studies on the F1 hybrid of Chrysanthemum lineare (now Leucanthemella lineare) × Ch. nipponicum (now Nipponanthemum nipponicum). Zeitschrift fűr Vererbungslehre 92:190–196

    CAS  PubMed  Google Scholar 

  • Tanaka R, Shimotomai N (1968) A Cytogenetic Study on the F1 Hybrid of Chrysanthemum makinoi × Ch. vulgare (now Tanacetum vulgare). Cytologia 33:241–245

    Article  Google Scholar 

  • Tang FP, Chen FD, Chen SM, Teng NJ, Fang WM (2009) Intergeneric hybridization and relationship of genera within the tribe Anthemideae Cass. (Chrysanthemum crassum (Kitam.) Kitam. x Crossostephium chinense (L.) Makino). Euphytica 169:133–140

    Article  CAS  Google Scholar 

  • Tang FP, Chen FD, Chen SM, Wang X, Zhao HB (2010a) Molecular cytogenetic identification and relationship of the artificial intergeneric hybrid between Dendranthema indica and Crossostephium chinense by GISH. Plant Syst Evol 289:91–99

    Article  Google Scholar 

  • Tang FP, Chen SM, Deng YM, Chen FD (2010b) Intergeneric hybridization between Dendranthema crassum and Ajania myriantha. Acta Hortic 855:267–272

    Google Scholar 

  • Tang FP, Wang H, Chen SM, Chen FD, Liu Z, Fang WM (2011) Intergeneric hybridization between Dendranthema nankingense and Tanacetum vulgare. Sci Hortic 132:1–6

    Article  CAS  Google Scholar 

  • Vallès J, Torrell M, Garnatje T, Garcia-Jacas N, Vilatersana R, Susanna A (2003) Genus Artemisia and its allies, phylogeny of the subtribe Artemisinae (Asteraceae, Anthemideae) based on nucleotide sequences of nuclear ribosomal DNA internal transcribed spacers (ITS). Plant Biol 5:274–284

  • Wagstaff SJ, Breitwieser I (2002) Phylogenetic relationships of New Zealand Asteraceae inferred from ITS sequences. Plant Syst Evol 231(1–4):203–224

    Article  CAS  Google Scholar 

  • Wang HY, Chai Y, Chu X, Zhao Y, Wu Y, Zhao J, Ngezahayo F, Xu C, Liu B (2009) Molecular characterization of a rice mutator-phenotype derived from an incompatible cross-pollination reveals transgenerational mobilization of multiple transposable elements and extensive epigenetic instability. BMC Plant Biol 9:63. doi:10.1186/1471-2229-9-63

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Watson LE, Evans TM, Boluarte T (2000) Molecular phylogeny and biogeography of tribe Anthemideae (Asteraceae), based on chloroplast gene ndhF. Mol Phylogenet Evol 15:59–69

    Article  CAS  PubMed  Google Scholar 

  • Watson LE, Bates PL, Evans TM, Unwin MM, Estes JR (2002) Molecular phylogeny of Subtribe Artemisiinae (Asteraceae), including Artemisia and its allied and segregate genera. BMC Evol Biol 2:17–29

    Article  PubMed Central  PubMed  Google Scholar 

  • Yang D, Hu X, Liu Z, Zhao H (2010) Intergeneric hybridizations between Opisthopappus taihangensis and Chrysanthemum lavandulifolium. Sci Hortic 125:718–723

    Article  CAS  Google Scholar 

  • Zhao HB, Chen FD, Chen SM, Wu GS, Guo WM (2010) Molecular phylogeny of Chrysanthemum, Ajania and its allies (Anthemideae, Asteraceae) as inferred from nuclear ribosomal ITS and chloroplast trnL-F IGS sequences. Plant Syst Evol 284:153–169

    Article  CAS  Google Scholar 

  • Zheng Y, Shen J, An Y, Zhang J, Rao G, Zhang Q, Zhao H (2013) Intergeneric hybridization between Elachanthemum intricatum (Franch.) Ling et Ling and Opisthopappus taihangensis (Y. Ling) C. Shih. Genet Resour Crop Evol 60:473–482

    Article  Google Scholar 

  • Zhu WY, Jiang JF, Chen SM, Wang L, Xu L, Wang H, Guan ZY, Chen FD (2013) Intergeneric hybrid between Chrysanthemum ×morifolium and Artemisia japonica achieved via embryo rescue shows salt tolerance. Euphytica 191:109–119

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by National “Twelfth Five-Year” Plan for Science & Technology Support (Grant No. 2012BAD01B07). We are thankful to Prof. Guangyuan Rao, Prof. Conglin Huang, Dr. Fusheng Yang and Dr. Chang Luo for their excellent technical support. We would like to express our sincere thank to Mr. Jaap Spaargaren and anonymous reviewers for their revising on this paper.

Author information

Authors and Affiliations

  1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, Haidian, Beijing, 100083, China

    Xiaobo Wu, Ge Hong, Fei Xie & Huien Zhao

  2. National Engineering Research Center for Floriculture, Haidian, Beijing, 100083, China

    Xiaobo Wu, Ge Hong, Fei Xie & Huien Zhao

  3. College of Landscape Architecture, Beijing Forestry University, Haidian, Beijing, China

    Xiaobo Wu, Ge Hong, Fei Xie & Huien Zhao

  4. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Haidian, Beijing, 100093, China

    Yanyan Liu

  5. College of Biological Sciences, China Agricultural University, Haidian, Beijing, 100193, China

    Zhaohui Liu

  6. Institute of Breeding of Beijing Summer Chrysanthemum, Shunyi, Beijing, 101300, China

    Wenchao Liu

Authors
  1. Xiaobo Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ge Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanyan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fei Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhaohui Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wenchao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Huien Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Huien Zhao.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wu, X., Hong, G., Liu, Y. et al. Possible intergeneric hybridization between Cancrinia maximowiczii C. Winkl. and Chrysanthemum naktongense (Nakai) Tzvel. × C. ×morifolium Ramat. ‘Aifen’. Genet Resour Crop Evol 62, 293–301 (2015). https://doi.org/10.1007/s10722-014-0153-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-014-0153-y

Keywords

Search

Navigation