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Obtusifoliaceae, a new family of leafy liverworts to accommodate Konstantinovia, newly described from the Hengduan Mts. (South China) and Obtusifolium (Cephaloziineae, Marchantiophyta)

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Abstract

The study considers the phylogenetic affinities and taxonomical placement of the previously unknown hepatic from Hengduan Mts. in Yunnan Province of China. This area is known to house a vast assemblage of peculiar and taxonomically secluded taxa of hepatics, serving as kind of storehouse of missing links in the evolution. The newly recorded taxon combines lophozioid appearance with prominent tooth in the antical side of the leaf—the feature previously observed in Cephaloziineae very rarely and in bracts only. Molecular phylogenetic reconstruction, inferred from the nuclear ITS region and the chloroplast rps4 gene, trnL-F region and trnG-intron resolved Konstantinovia gen. nov. as the closest relative of highly phylogenetically isolated monospecific genus Obtusifolium. Their joint clade is sister to the Anastrophyllaceae clade in the tree inferred from combined ITS and trnL sequences, while topology inferred from combined trnG and rps4 sequences fails to resolve its affinity within the Scapaniaceae s.l. clade. Therefore, the new family Obtusifoliaceae is introduced to accommodate these genera, and the familial classification of Cephaloziineae is discussed.

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Availability of data and materials (data transparency)

The material was legally collected by the permission from Botanical Garden in Kunming and parts of collected material was transferred abroad (to Russian Federation) with corresponding permissions. The studied material is now available in VBGI, KUN and MW.

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Acknowledgements

The work was partially supported by the Russian Foundation for Basic Research (grant 20-04-00278 for VB). The work of VF was supported by MSU research project no. AAAA-A16-116021660039-1, the work of AF was partly supported by the institutional research project no. 118021490111–5. The line drawings were provided by Mr. Matvei Bakalin to whom authors are sincerely grateful.

Funding

Russian Foundation for Basic Research (grant 20-04-00278 for VB), Moscow State University (project no. AAAA-A16-116021660039-1 for VF), Main Botanical Garden institutional research project (no. 118021490111–5 for AF) and Botanical Garden-Institute research project (no. АААА-А20-120031990012-4 for VB).

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Authors and Affiliations

  1. Laboratory of Cryptogamic Biota, Botanical Garden-Institute FEB RAS, Makovskogo Street 142, Vladivostok, Russia, 690024

    Vadim A. Bakalin & Vladimir E. Fedosov

  2. Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory Street 1–12, Moscow, Russia, 119234

    Vladimir E. Fedosov

  3. Tsitsin Main Botanical Garden , RAS, Botanicheskaya Street 4, Moscow, Russia, 127276

    Alina V. Fedorova

  4. Herbarium, Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China

    Wen Zhang Ma

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  1. Vadim A. Bakalin
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  2. Vladimir E. Fedosov
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  3. Alina V. Fedorova
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  4. Wen Zhang Ma
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Contributions

VB and VF contributed to general concept manuscript writing, VF and AF contributed to molecular analysis, VB and VF contributed to morphological implications, and VB and MWZ contributed to plant collecting.

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Correspondence to Vadim A. Bakalin.

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Online resource 1. a Specimens and GenBank accession numbers used in reconstruction based on rps4 + trnG sequences (newly obtained sequences are provided in bold). b Specimens and GenBank accession numbers used in reconstruction based on ITS + trnLF sequences (newly obtained sequences are provided in bold). c Voucher data of the specimens originally studied for molecular phylogenetic reconstructions.

Online resource 2. The combined alignment ITS & trnL-F dataset.

Online resource 3. The combined alignment rps4 & trnG dataset.

Online resource 4. h ML tree inferred from rps4 & trnG dataset, indels are coded using simple indel coding technique. i ML tree inferred from rps4 & trnG dataset, indels are not considered. j ML tree inferred from ITS & trnL-F dataset, indels are coded using simple indel coding technique. k ML tree inferred from ITS & trnL-F dataset, indels are not considered. l ML tree inferred from ITS dataset, indels are not considered.

Online resource 5. Konstantinovia pulchra Bakalin & Fedosov: a the cushion in dry condition (holotype, VBGI); b plant habit, vital (holotype, VBGI); c plant habit, vital (holotype, VBGI); d plant habit, vital (holotype, VBGI); e gemmae (holotype, VBGI); f gemmae (holotype, VBGI); g midleaf cells showing oil bodies, vital (holotype, VBGI).

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Bakalin, V.A., Fedosov, V.E., Fedorova, A.V. et al. Obtusifoliaceae, a new family of leafy liverworts to accommodate Konstantinovia, newly described from the Hengduan Mts. (South China) and Obtusifolium (Cephaloziineae, Marchantiophyta). Plant Syst Evol 307, 62 (2021). https://doi.org/10.1007/s00606-021-01779-8

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