Skip to main content
SpringerLink
Log in

Phylogeny and evolution of Baptistonia (Orchidaceae, Oncidiinae) based on molecular analyses, morphology and floral oil evidences

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Baptistonia is endemic to the Brazilian Atlantic Forest and is made up of 23 species, the phylogenetic relationships of which are analysed here. Three plastid regions (psbA-trnH, rpoB-trnC and trnS-G) were sequenced. Using seven inter-simple sequence repeat (ISSR) markers, we scored 183 loci of ISSR dominant markers. Trees were obtained by successively using (a) maximum parsimony and Bayesian analysis of individual and combined sequence data sets, (b) neighbour joining (NJ) and Dollo parsimony of the ISSR data and (c) NJ analysis of the floral oil composition. These approaches, as well as the results of a previous morphological analysis, were combined using a supertree method, yielding clearer relationships within the genus. Various results of our study indicate that the Baptistonia species are recently radiated. B. colorata and B. venusta (previously known as Oncidium trulliferum) are confirmed as being sister to the remaining species of the genus. Four taxonomic groups are clearly inferred. The evolution of some morphological traits and floral oil composition proved to be strongly linked to the phylogeny.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1a–d
Fig. 2a–f
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Archibald JK, Mort ME, Crawford DJ, Santos-Guerra A (2006) Evolutionary relationships within recently radiated taxa: comments on methodology and analysis of inter-simple sequence repeat data and other hypervariable, dominant markers. Taxon 55(3):747–756

    Google Scholar 

  • Barbosa Rodrigues J (1877) Genera et Species Orchidacearum Novarum, vol 1. p 95

  • Bininda-Emonds ORP, Bryant HN (1998) Properties of matrix representation with parsimony analyses. Syst Biol 47:497–508

    PubMed  CAS  Google Scholar 

  • Buchmann SL (1987) The ecology of oil flowers and their bees. Annu Rev Ecol Syst 18:343–369

    Article  Google Scholar 

  • Bussell JD, Waycott M, Chappill JA (2005) Arbitrarily amplified DNA markers as characters for phylogenetic inference. Perspect Plant Ecol Evol Syst 7:3–26

    Article  Google Scholar 

  • Castro Neto VP (2007) O gênero Baptistonia. In: Anais do 2° Congresso Oriental/Brasil Orquideas, 7–10 June 2007. Editora Brasil, São Paulo, pp 60–68

  • Chase MW (1986) A reappraisal of the oncidioid orchids. Syst Bot 11(3):477–491

    Article  Google Scholar 

  • Chase MW, Hanson L, Albert VA, Whitten WM, Williams NH (2005) Life history evolution and genome size in subtribe Oncidiinae (Orchidaceae). Ann Bot 95(1):191–199

    Article  PubMed  CAS  Google Scholar 

  • Chiron G (2007a) Note sur la distribution géographique de Baptistonia Barbosa Rodrigues (Orchidaceae, Oncidiinae). Richardiana 7(4):145–173

    Google Scholar 

  • Chiron G (2007b) Phylogenetic analyses of the genus Baptistonia (Orchidaceae: Oncidiinae) sensu lato based on morphological characters. J Bot Res Inst Tex 1(2):913–931

    Google Scholar 

  • Chiron G (2008) Baptistonia Barbosa Rodrigues (Orchidaceae, Oncidiinae)—Notes taxinomiques 2. Richardiana 8(3):111–125

    Google Scholar 

  • Chiron G, Castro Neto VP (2004) Contribution à la connaissance des orchidées du Brésil. III—Rétablissement du genre Baptistonia Barbosa Rodrigues. Richardiana 4(3):109–120

    Google Scholar 

  • Clements MA, Jones DL, Sharma IK, Nightingale ME, Garratt MJ, Fitzgerald KJ, Mackenzie AM, Molloy BPJ (2002) Phylogenetics of Diurideae (Orchidaceae) based on the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA. Lindleyana 17(3):135–171

    Google Scholar 

  • Cogniaux A (1906) Orchidaceae III. In: Martius CFP (ed) Flora brasiliensis, vol 3, part 6. Otto Koeltz Science Publishers, Koenigstein, p 339

    Google Scholar 

  • Creevey CJ, McInerney JO (2005) Clann: investigating phylogenetic information through supertree analyses. Bioinformatics 21(3):390–392

    Article  PubMed  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Dressler RL (1993) Phylogeny and classification of the orchid family. Dioscorides Press, Portland, OR, pp 177–181

    Google Scholar 

  • Endress PK (1994) Diversity and evolutionary biology of tropical flowers. Cambridge University Press, Cambridge, pp 385–386

    Google Scholar 

  • Erixon P, Svennblad B, Britton T, Oxelman B (2003) Reliability of Bayesian posterior probabilities and bootstrap frequencies in phylogenetics. Syst Biol 52(5):665–673

    Article  PubMed  Google Scholar 

  • Faria A (2004) Sistematica filogenetica e delimitação dos generos da subtribo Oncidiinae. PhD thesis, Universidad Estadual de Campinas, Campinas

  • Farris JS, Albert VA, Källersjö M, Lipscomb D, Kluge AG (1996) Parsimony jackknifing outperforms neighbor-joining. Cladistics 12:99–124

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Hammer O, Harper DAT, Ryan PD (2007) PAST, PAlaeontological STatistics, ver. 1.73. 4 September 2007

  • Hao G, Lee D-H, Lee JS, Lee NS (2002) A study of taxonomical relationships among species of Korean Allium sect. Sacculiferum (Alliaceae) and related species using inter-simple sequence repeat (ISSR) markers. Bot Bull Acad Sin 43:63–68

    CAS  Google Scholar 

  • Joshi SP, Gupta VS, Aggarwal RK, Ranjekar PK, Brar DS (2000) Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor Appl Genet 100:1311–1320

    Article  CAS  Google Scholar 

  • Kraenzlin F (1922) Orchidaceae-Monandrae. Tribus Oncidiinae-Odontoglosseae pars II. In: Engler A (ed) Das Pflanzenreich. Regni vegetabilis conspectus. W. Engelmann, Leipzig

    Google Scholar 

  • Kress WJ, Prince LM, Williams KJ (2002) The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. Am J Bot 89(11):1682–1696

    Article  CAS  Google Scholar 

  • Moore BR, Smith SA, Donoghue MJ (2006) Increasing data transparency and estimating phylogenetic uncertainty in supertrees: approaches using nonparametric bootstrapping. Syst Biol 55(4):662–676

    Article  PubMed  Google Scholar 

  • Mort ME, Archibald JK, Randle CP, Levsen ND, O’Leary TR, Topalov K, Wiegand CM, Crawford DJ (2007) Inferring phylogeny at low taxonomic levels: utility of rapidly evolving cpDNA and nuclear ITS loci. Am J Bot 94(2):173–183

    Article  Google Scholar 

  • Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University

  • Pabst GF, Dungs F (1977) Orchidaceae brasilienses II. Brücke-Verlag Kurt Schmersow, Hildesheim

    Google Scholar 

  • Ramalho M (2004) Stingless bees and mass flowering trees in the canopy of Atlantic Forest: a tight relationship. Acta Bot Bras 18(1):37–47

    Article  Google Scholar 

  • Reis MG (2005) Caracteres químicos em estudos de filogenia e biologia de polinização de espécies de Oncidiinae (Orchidaceae). Universidad Estadual de Campinas, Campinas

    Google Scholar 

  • Reis MG, de Faria AD, Bittrich V, Amaral M, Marsaioli AJ (2000) The chemistry of flower rewards—Oncidium (Orchidaceae). J Braz Chem Soc 11(6):600–608

    Article  CAS  Google Scholar 

  • Ronquist F (1996) Matrix representation of trees, redundancy, and weighting. Syst Biol 45:247–253

    Google Scholar 

  • Ronquist F, Huelsenbeck JP, van den Mark P (2005) MrBayes 3.1 Manual. Home page at: http://mrbayes.net

  • Roubik DW (1989) Ecology and natural history of tropical bees. Cambridge University Press, Cambridge

    Google Scholar 

  • Ryan A, Whitten WM, Johnson MAT, Chase MW (2000) A phylogenetic assessment of Lycaste and Anguloa (Orchidaceae: Maxillarieae). Lindleyana 15(1):33–45

    Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Salamin N, Hodkinson TR, Savolainen V (2002) Building supertrees: an empirical assessment using the grass family (Poaceae). Syst Biol 51(1):136–150

    Article  PubMed  Google Scholar 

  • Sanderson MJ, Purvis A, Henze C (1998) Phylogenetic supertrees: assembling the trees of life. Trends Ecol Evol 13:105–110

    Article  Google Scholar 

  • Senghas K (1997) In: Sonderabdruck aus Schlechter, Die Orchideen, 3 Auflage—76. Subtribus Oncidiinae. Blackwell Wissenschaftsverlag, Berlin

  • Shaw J, Lickey EB, Beck JT, Farmer SB, Liu W, Miller J, Siripun KC, Winder CT, Schilling EE, Small RL (2005) The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. Am J Bot 92(1):142–166

    Article  CAS  Google Scholar 

  • Simmons MP, Zhang L-B, Webb CT, Müller K (2006) A penalty of using anonymous dominant markers (AFLPs, ISSRs, and RAPDs) for phylogenetic inference. Mol Phylogenet Evol 42:528–542

    Article  PubMed  Google Scholar 

  • Singer RB, Cocucci AA (1999) Pollination mechanisms in four sympatric southern Brazilian Epidendroideae orchids. Lindleyana 14(1):47–56

    Google Scholar 

  • Staden R (1996) The Staden sequence analysis package. Mol Biotechnol 5:233–241

    Article  PubMed  CAS  Google Scholar 

  • Sudupak MA (2004) Inter and intra-species inter simple sequence repeat (ISSR) variations in the genus Cicer. Euphytica 135:229–238

    Article  CAS  Google Scholar 

  • Swofford DL (1998) PAUP*. Phylogenetic analysis using parsimony (*and other methods), version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • Toscano de Brito ALV (2001) Systematic review of the Ornithocephalus group (Oncidiinae; Orchidaceae) with comments on Hofmeisterella. Lindleyana 16(3):157–217

    Google Scholar 

  • Tremetsberger K, Stuessy TF, Kadlec G, Urtubey E, Baeza CM, Beck SG, Valdebenito HA, de Fatima Ruas C, Matzenbacher NI (2006) AFLP phylogeny of South American species of Hypochaeris (Asteraceae, Lactuceae). Syst Bot 31(3):610–626

    Google Scholar 

  • van den Berg C, Higgins WE, Dressler RL, Whitten WM, Soto Arenas MA, Culham A, Chase MW (2000) A phylogenetic analysis of Laeliinae (Orchidaceae) based on sequence data from internal transcribed spacers (ITS) of nuclear ribosomal DNA. Lindleyana 15(2):96–114

    Google Scholar 

  • van den Berg C, Ryan A, Cribb PJ, Chase MW (2002) Molecular phylogenetics of Cymbidium (Orchidaceae: Maxillarieae): sequence data from internal transcribed spacers (ITS) of nuclear ribosomal DNA and plastid matK. Lindleyana 17(2):102–111

    Google Scholar 

  • Vogel S (1969) Flowers offering fatty oil instead of nectar. In: Abstracts of the XI International Botany Congress, Seattle (abstract no. 229)

  • Williams NH (1978) A preliminary bibliography on euglossine bees and their relationship with orchids and other plants. Selbyana 2:345–355

    Google Scholar 

  • Williams NH, Chase MW, Fulcher T, Whitten WM (2001) Molecular systematics of the Oncidiinae based on evidence from four DNA sequence regions: expanded circumscriptions of Cyrtochilum, Erycina, Otoglossum, and Trichocentrum and a new genus (Orchidaceae). Lindleyana 16:113–139

    Google Scholar 

  • Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20:176–183

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are indebted to Vitorino P. Castro Neto, Eduardo Catharino, Thomas Adamski, Angelo Ebani, Savio Caliman, Sergio Gutfreund, Sidney Marçal, Paulo Uhl, Carlos Régent (all from Brazil), as well as Dominique Barthelemy, Michel Frey (France) and ‘Edgardo’ (Paraguay), for helping us with the collection of the materials.

Author information

Authors and Affiliations

  1. Herbiers, Université de Lyon, 69622, Villeurbanne Cedex, France

    Guy R. Chiron

  2. Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Av. Transnordestina s/n, 44036-900, Feira de Santana, Bahia, Brazil

    R. Patricia Oliveira, Tarciso M. Santos & Cassio van den Berg

  3. Centre d’Etude des Substances Naturelles, UMR 5557 Ecologie Microbienne, Université de Lyon, 69622, Villeurbanne Cedex, France

    Florian Bellvert & Cédric Bertrand

Authors
  1. Guy R. Chiron
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Patricia Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tarciso M. Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Florian Bellvert
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cédric Bertrand
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cassio van den Berg
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guy R. Chiron.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (XLS 106 kb)

Appendix: samples used in DNA sequencing, ISSR scoring and floral oils analyses (all vouchers deposited in LY except JBSP vouchers deposited in SP)

Appendix: samples used in DNA sequencing, ISSR scoring and floral oils analyses (all vouchers deposited in LY except JBSP vouchers deposited in SP)

Symbols: samples were used for psbA-trnH (P), rpoB-trnC (R), trnS-G (T) sequencing, ISSR scoring (I) and/or floral oil analysis (O).

The numbers following the letters P, R and T represent the GenBank numbers relating to sequences.

Baptistonia albinoi (Schltr.) Chiron & V.P. Castro: GC0084 (I, O), GC0085 (I, O), GC0086 (I), GC2578 (P: EU935673, R: EU935643, T: EU935705, I). Baptistonia brieniana (Rchb.f.) V.P. Castro & Chiron: GC0001 (P: EU935669, R: EU935640, T: EU935701, I), GC2676 (I, O). Baptistonia colorata (Königer & J.G. Weinmann) Chiron: GC0025 (P: EU935667, R: EU935639, T: EU935699, I), GC0026 (I), GC0062 (I). Baptistonia cornigera (Lindl.) Chiron & V.P. Castro: GC2459 (P: EU935680, R: EU935650, T: EU935712, O), GC2460 (I), GC2462 (O), GC2463 (I), GC3052 (I), GC3062 (I), GC3074 (I). Baptistonia cruciata (Rchb.f.) V.P. Castro & Chiron: GC0030 (I), GC0032 (I), GC0037 (I), GC2866 (I), GC2870 (P: EU935690, R: EU935658, T: EU935721, O), GC2876 (O), MF1058 (I). Baptistonia damacenoi Chiron & V.P. Castro: GC0007 (O), GC0008 (O), GC2273 (I), GC2586 (O), GC2768 (O), MF1047 (P: EU935674, R: EU935644, T: EU935706, I, O). Baptistonia aff damacenoi: GC0014 (P: EU935687, R: EU935655, T: EU935719, O). Baptistonia echinata Barb.Rodr.: GC0002 (O), GC0035 (I, O), GC0036 (O), GC0042 (T: EU935709, I), GC0043 (I), GC0073 (I), GC2582 (P: EU935677, R: EU935647), GC3045 (I), GC3055 (O). Baptistonia gutfreundiana (Chiron & V.P. Castro) Chiron & V.P. Castro: GC0047 (P: EU935678, R: EU935648, T: EU935710, O), GC2579 (I), GC2952 (I), GC2956 (I), GC2959 (I), GC3037 (O), GC3060 (I, O). Baptistonia kautskyi (Pabst) V.P. Castro & Chiron: GC2740 (I), GC2743 (I, O), GC3071 (I, O), MF741 (I), MF1079 (P: EU935686, R: EU935654, T: EU935718, I). Baptistonia leinigii (Pabst) V.P. Castro & Chiron: GC0038 (P: EU935684), GC0101 (R: EU935652, T: EU935716, I), GC3113 (I, O), GC3115 (I), GC3117 (I), GC3136 (I). Baptistonia lietzei (Regel) Chiron & V.P. Castro: GC0041 (P: EU935689, R: EU935657, T: EU935720), GC2283 (I), GC3067 (O), GC3068 (I, O), GC3069 (O), GC3073 (I), GC3085 (I), JBSP12275 (I). Baptistonia lietzei (Regel) Chiron & V.P. Castro subsp. guairensis Chiron: GC2656 (I, O), GC2657 (I), GC2693 (I), GC2703 (I, O), GC2675 (I). Baptistonia aff. lietzei (Regel) Chiron & V.P. Castro: JBSP6554 (I), GC0048 (P: EU935688, R: EU935656). Baptistonia nitida (Barb.Rodr.) V.P. Castro & Chiron: GC0021 (P: EU935685, R: EU935653, T: EU935717, I), GC0023 (I), GC0040 (O), GC2591A (I), GC2591B (O), GC2591E (I), GC2593 (O), GC3084 (I). Baptistonia pabstii (Campacci & C. Espejo) V.P. Castro & Chiron: GC3042 (R: EU935642, T: EU935703, I, O), GC3059 (P: EU935671, I, O). Baptistonia pubes (Lindl.) Chiron & V.P. Castro: GC0005 (O), GC2241 (I, O), GC2768 (I), GC3034 (I), GC3036 (I), GC3038 (P: EU935681, R: EU935651, T: EU935713), GC3048 (I), GC3070 (I, O). Baptistonia pulchella (Regel) Chiron & V.P. Castro: GC2861 (I), GC2882 (P: EU935692, R: EU935660, T: EU935723, I). Baptistonia riograndensis (Cogn.) V.P. Castro & Chiron: GC0105 (P: EU935672, I, O), GC0106 (I, O), GC3114 (I), GC3130 (T: EU935704), GC3134 (I), GC3137 (I). Baptistonia sarcodes (Lindl.) Chiron & V.P. Castro: GC0017 (P: EU935682, T: EU935714, I, O), GC2242 (I, O), GC2764 (I, O), GC2801 (I), JBSP.A492 (I). Baptistonia silvana (V.P. Castro & Campacci) V.P. Castro & Chiron: GC0022 (P: EU935676, R: EU935646, I, O), GC0057 (I), GC2942 (T: EU935708, O), GC3072 (I, O), VDB1573 (I), VDB1574 (I). Baptistonia truncata (Pabst) Chiron & V.P. Castro: GC2240 (I, O), GC2785 (I), GC2824 (I), GC2840 (O), GC2899A (P: EU935691, R: EU935659, T: EU935722, I), GC2899B (I). Baptistonia uhlii Chiron & V.P. Castro: GC2689 (P: EU935675, R: EU935645, T: EU935707, I, O), GC2689A (I), GC3094 (I), GC3095 (I). Baptistonia velteniana V.P. Castro & Chiron: GC0028 (R: EU935641, I), GC0029 (P: EU935670, T: EU935702, I). Baptistonia venusta (Drapiez) Chiron: GC0107 (I), GC0108 (O), GC0109 (O), GC2850 (P: EU935668, T: EU935700), JBSP3273 (I), JBSP7028 (I), JBSP11008 (I). Baptistonia widgrenii (Lindl.) V.P. Castro & Chiron: GC0112 (I), GC2574 (I), GC2577 (P: EU935679, R: EU935649, T: EU935711, I, O), GC2581 (I, O). Baptistonia lietzei × cornigera: GC0079 (P: EU935683, T: EU935715). Gomesa crispa Klotzsch & Rchb. f.: GC2803 (O). Gomesa laxiflora (Lindl.) Klotzsch & Rchb. f.: GC2747 (P: EU935661, T: EU935693, O). Oncidium barbatum Lindl.: GC0055 (P: EU935662, R: EU935635, T: EU935694). Oncidium concolor Hook.: GC2847 (P: EU935664, R: EU935637, T: EU935696), GC2849 (O). Oncidium croesus Rchb. f.: GC2239 (P: EU935663, R: EU935636, T: EU935695). Oncidium gardneri Lindl.: GC0054 (P: EU935666, R: EU935638, T: EU935697). Oncidium hydrophilum Barb.Rodr.: GC2978 (P: EU935665). Oncidium marshallianum Rchb. f.: GC2779 (O). Oncidium ouricanense V.P. Castro & Campacci: GC0083 (T: EU935698). Rodrigueziella handroi (Hoehne) Pabst: GC2891 (O).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chiron, G.R., Oliveira, R.P., Santos, T.M. et al. Phylogeny and evolution of Baptistonia (Orchidaceae, Oncidiinae) based on molecular analyses, morphology and floral oil evidences. Plant Syst Evol 281, 35–49 (2009). https://doi.org/10.1007/s00606-009-0181-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-009-0181-9

Keywords

Search

Navigation