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Species identification of the psammophilous tenebrionid beetles Phaleria acuminata Küster, 1852 and Phaleria bimaculata (Linnaeus, 1767) from central Mediterranean beaches: geometric morphometrics and molecular insights from species to population level

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Abstract

Dominating global arid environments, from desert to coastal dunes, most Tenebrionidae are highly specific in their habitat preferences and display limited dispersal potential, thus exhibiting a remarkable degree of regional genetic and morphological differentiation. The tenebrionid genus Phaleria is speciose and widely distributed, with P. acuminata and P. bimaculata having a wide Mediterranean distribution, with numerous morphological differentiations at population level, often described as different taxa of doubtful taxonomical significance. In order to investigate the variability of the central Mediterranean populations of P. bimaculata and P. acuminata and to compare the results obtained with different identification techniques, these species were sampled on sandy beaches in Sicily (southern Italy) and on circum-Sicilian and Maltese islands. Collected samples were studied through the application of geometric morphometrics and the sequencing of a fragment of the mitochondrial COII gene. Geometric morphometrics and molecular analyses gave congruent results, allowing a sound separation of the two species. At the population level, the two species showed different patterns. P. acuminata showed a remarkable morphological and molecular homogeneity throughout the sampled area. Conversely, two well-characterized sub-clades were detected within P. bimaculata, and within the two lineages, a low-to-absent inter-populations differentiation was observed, in spite of the physical isolation of the sampled sandy beaches and of their geographical distance. These two P. bimaculata lineages, hereby named “Tyrrhenian sub-clade” and “Southern sub-clade,” might be compatible with the hypothesis of subspecific status already proposed for the populations from the Aeolian archipelago (as P. bimaculata marcuzzii Aliquò).

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References

  • Adams DC, Rohlf FJ, Slice D (2004) Geometric morphometrics: ten years of progress following the ‘revolution’. Ital J Zool 71:5–16

    Article  Google Scholar 

  • Aliquò V(1993) Dati nuovi e riassuntivi sui coleotteri Tenebrionidi delle isole circumsiciliane (Coleoptera: Tenebrionidae). Naturalista sicil, Palermo 17:p 111–125

    Google Scholar 

  • Aliquò V, Leo P (1997–1998) I coleotteri tenebrionidi della regione iblea (Sicilia sudorientale). Atti e Memorie dell’Ente Fauna Siciliana vol.V: 49–84

  • AliquòV Soldati F (2010) Coleotteri Tenebrionidi di Sicilia (Insecta: Coleoptera Tenebrionidae). Monografie Naturalistiche, 1. Edizioni Danaus, Palermo, p 176

    Google Scholar 

  • Bookstein FL (1989) Principal warps: thin-plate splines and the decomposition of deformations. IEEE Trans Pattern Anal Mach Intell 11:567–585

    Article  Google Scholar 

  • Bookstein FL (1991) Morphometric tools for Landmark data: geometry and biology. Biology Cambridge University Press, Cambridge

    Google Scholar 

  • Canzoneri S (1968) Materiali per una monografia delle Phaleria del sottogenere Phaleria Latr. Memorie della Società Entomologica Italiana 47:117–167

    Google Scholar 

  • Canzoneri S (1970) I Tenebrionidi delle Isole Egadi. Boll Museo civ St nat Verona 18:55–89

    Google Scholar 

  • ClarkinE Maggs CA, Arnott A, Briggs S, Houghton JDR (2012) The colonization of macroalgal rafts by the genus Idotea (sub-phylum Crustacea; order Isopoda): an active or passive process? J Mar Biol Ass UK 92:1273–1282

    Article  Google Scholar 

  • Colombini I, Chelazzi L, Fallaci M, Palesse L (1994) Zonation and surface activity of some Tenebrionid beetles living on a Mediterranean sandy beach. J Arid Environ 28:215–230

    Article  Google Scholar 

  • Contreras-Diaz HG, Moya O, Oromi P, Juan C (2003) Phylogeography of the endangered darkling beetle species of Pimelia endemic to Gran Canaria (Canary Islands). Mol Ecol 12:2131–2143

    Article  CAS  Google Scholar 

  • Dajoz R (1984) Tenebrionidae européens noveaux ou peu connus. Cahiers du Naturaliste 39:89–92

    Google Scholar 

  • de Jong YSDM (ed) (2010) Fauna Europaea version 2.3. Web Service available online at http://www.faunaeur.org

  • Deidun A, Schembri PJ (2008) Assessing inter-beach differences in semi-terrestrial arthropod assemblages on Maltese pocket sandy beaches (Central Mediterranean). Mar Ecol 29:108–117

    Article  Google Scholar 

  • Deidun A, Azzopardi M, Saliba S, Schembri PJ (2003) Low faunal diversity on Maltese sandy beaches: fact or artefact? Est Coast Shelf Sci 58:83–92

    Article  Google Scholar 

  • Deidun A, Saliba S, Schembri PJ (2007) Banquette faunal assemblages from groomed and ungroomed beaches in the Maltese Islands. In: Proceedings of the CIESM 38th congress, Istanbul, p 456

  • Deidun A, Saliba S, Schembri PJ (2009) Considerations on the ecological role of wrack accumulations on sandy beaches in the Maltese Islands and recommendations for their conservation management. J Coast Res (SI). In: Proceedings of the 10th international coastal symposium, vol 56. Portugal, pp 410–414

  • Deidun A, Pawley A, Schembri PJ (2010) Distribution and biology of two sympatric Phaleria (Coleoptera: Tenebrionidae) species on Maltese sandy beaches. In: Proceedings of the 39th CIESM Congress, Venice, Italy, p. 737

  • Deidun A, Marrone F, Sparacio I, Arculeo M, Curatolo T, Sciberras A, Sciberras J, Lo Brutto S (2011) New morphological, morphometric and distribution data on Phaleria bimaculata (Linnaeus, 1767) (Coleoptera: Tenebrionidae) from the islands and archipelagos of the Central Mediterranean area. Bull Insect 64:215–222

    Google Scholar 

  • Doyen JT, Matthews EG, Lawrence JF (1989) Classification and annotated checklist of the Australian genera of Tenebrionidae (Coleoptera). Invertebr Syst 3(3):229–260

    Article  Google Scholar 

  • Fallaci M, Colombini I, Palesse L, Chelazzi L (1997) Spatial and temporal strategies in relation to environmental constraints of four tenebrionids inhabiting a Mediterranean coastal dune system. J Arid Environ 37:45–64

    Article  Google Scholar 

  • Fattorini S (2002) A comparison of relict versus dynamic models for tenebrionid beetles (Coleoptera: Tenebrionidae) of Aegean Islands (Greece). Belgian J Zool 132:55–64

    Google Scholar 

  • Fattorini S, Leo P (2000a) Levels of endemism in the Aegean tenebrionids (Coleoptera, Tenebrionidae). Biogeographia Lav Soc ital Biogeogr 21:429–440

    Google Scholar 

  • Fattorini S, Leo P (2000b) Darkling beetles from Mediterranean minor islands: new records and biogeographical notes. Boll Soc Entom ital 132:205–217

    Google Scholar 

  • Gardini G (1975) Materiali per lo studio dei Tenebrionidi dell’Arcipelago Toscano (Col. Heteromera). Lav Soc ital Biogeogr 5:637–723

    Google Scholar 

  • Gauci M, Deidun A, Schembri PJ (2005) Faunistic diversity of Maltese pocket sandy and shingle beaches: are these of conservation value? Oceanologia 27:219–241

    Google Scholar 

  • Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of phyml 3.0. Syst Bio 59:307–21

    Google Scholar 

  • Huelsenbeck JE, Ronquist F (2001) MrBayes: BAYESIAN inference of phylogeny. Biometrics 17:754–755

    CAS  Google Scholar 

  • Klingenberg CP (2011) MORPHOJ: an integrated software package for geometric Morphometrics. Mol Ecol Resour 11:353–357

    Article  PubMed  Google Scholar 

  • Lo Brutto S, Arculeo M, Grant WS (2011) Climate change and population genetic structure of marine species. Chem Ecol 27:107–119

    Article  Google Scholar 

  • Löbl I, Smetana A (eds) (2008) Catalogue of Palaearctic Coleoptera, vol 5. Tenebrionoidea. Apollo Books, Stenstrup, p 669

    Google Scholar 

  • Malanotte-Rizzoli P, Manca BB, D’Alcala MR, Theocharis A, Bergamasco A, Bregant D, Budillon G, Civitarese G, Georgopoulos D, Michelato A, Sansone E, Scarazzato P, Souvermezoglou E (1997) A synthesis of the Ionian hydrography, circulation and water mass pathways during POEM-phase I. Progr Oceanogr 39:153–204

    Article  Google Scholar 

  • Marcuzzi G (1996) Il melanismo nel genere Phaleria Latreille (Insecta, Coleoptera, Tenebrionidae). Bollettino della Accademia Gioenia di Scienze naturali, Catania 351:9–13

    Google Scholar 

  • Marrone F, Lo Brutto S, Arculeo M (2011) Cryptic invasion in Southern Europe: the case of Ferrissia fragilis (Pulmonata: Ancylidae) Mediterranean populations. Biologia 66:484–490

    Article  Google Scholar 

  • Mifsud D, Scupola A (1998) The Tenebrionidae (Coleoptera) of the Maltese Islands (Central Mediterranean). Annali del Museo Civico di Storia Naturale “G. Doria” 9:191–229

  • Millot C (1999) Circulation in the Western Mediterranean Sea. J Mar Syst 20:423–442

    Article  Google Scholar 

  • Minelli A, Chemini C, Argano R, Ruffo S (2002) La fauna in Italia. Touring Editore. Milano e Ministero dell’Ambiente e della Tutela del Territorio, Roma, p 448

    Google Scholar 

  • Nylander JAA (2004) MrModeltest v2. Evolutionary Biology Centre, Uppsala University, Program distributed by the author

    Google Scholar 

  • Pavesi L, Deidun A, De Matthaeis E, Tiedemann R, Ketmaier V (2012) Mitochondrial DNA and microsatellites reveal significant divergence in the beachflea Orchestia montagui (Talitridae: Amphipoda). Aquat Sci 74:587–596

    Article  CAS  Google Scholar 

  • Perić-Mataruga V, ProlićZ Nenadović V, Vlahović M, Mrdaković M (2008) The effect of a static magnetic field on the morphometric characteristics of neurosecretory neurons and corpora allata in the pupae of yellow mealworm Tenebrio molitor(Tenebrionidae). Int J RadiatBiol 84:1–8

    Article  Google Scholar 

  • Pinardi N, Arneri E, Crise A, Ravaioli M, Zavatarelli M (2005) The physical, sedimentary and ecological structure and variability of shelf areas in the Mediterranean Sea. In: Robinson AR, Brink KH (eds) The Sea vol. 14, chapter 32. Cambridge, Harvard University Press, pp 1243–1330

    Google Scholar 

  • Rohlf FJ (1993) Relative warp analysis and an example of its application to mosquito wings. In Contribution to Morphometrics. In: Marcus F, Bello E, García-Valdecasas A (eds) Contributions to morphometrics. Monografías del Museo Nacional de Ciencias Naturales, 8, Madrid, p 132–159

  • Rohlf FJ (1996) Morphometric spaces, shape components, and the effects of linear transformations. In:Marcus LF, Corti M, Loy A, Naylor GJP, Slice DE (eds)Advances in Morphometrics Nato ASI Series. Plenum Press, New York, pp 117–129

    Google Scholar 

  • Rohlf FJ (2004) tpsDIG 2, software available at http://life.bio.sunysb.edu/morph/

  • Rohlf FJ(2008) tpsUtil, software available at http://life.bio.sunysb.edu/morph/

  • Rohlf FJ, Marcus LF (1993) A revolution in Morphometrics. Trends Ecol Evol 8:129–132

    Article  Google Scholar 

  • Rohlf FJ, Slice DE (1990) Estensioni del metodo di Procuste per la sovrapposizione ottimale dei punti di riferimento. Sistematica Zoologica 39:40–59

    Google Scholar 

  • Sarà G, Milanese M, Prusina I, Sarà A, Angel DL, Glamuzina B, Nitzan T, Freeman S, Rinaldi A, Palmeri V, Montalto V, Lo Martire M, Gianguzza P, Arizza V, Lo Brutto S, De Pirro M, Helmuth B, Murray J, De Cantis S, Williams GA (2012) The impact of climate change on Mediterranean intertidal communities: losses in coastal ecosystem integrity and services. Regional Environmental Change (in press) DOI 10.1007/s10113-012-0360-z

  • Sheets HD (2003) IMP- Integrated Morphometrics Package. Department of Physics, Canisius College, Buffalo, New York. Available at:http://www2.canisius.edu/sheets/morphsoft.html

  • Taravati S, Darvish J, Mirshamsi O (2009) Geometric morphometric study of two species of the psammophilous genus Erodiontes (Coleoptera: Tenebrionidae) from the Lute desert, Central Iran. Iran J Anim Biosyst 5:81–89

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Viscosi V, Cardini A (2011) Leaves, taxonomy and geometric morphometrics: a simplified protocol for beginners. PLoS ONE 6(10):e25630

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zelditch M, Swiderski D, Sheets H, Fink W (2004) Geometric morphometrics for biologists: a primer. Elsevier Academic Press, London

    Google Scholar 

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Authors and Affiliations

  1. Department STeBICeF, Sezione di Biologia animale e Antropologia Biologica, University of Palermo, via Archirafi 18, 90123, Palermo, Italy

    Federico Marrone, Tiziana Curatolo, Marco Arculeo & Sabrina Lo Brutto

  2. IOI-MOC, University of Malta, Msida, MSD 2080, Malta

    Alan Deidun

Authors
  1. Federico Marrone
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  2. Alan Deidun
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  3. Tiziana Curatolo
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  4. Marco Arculeo
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  5. Sabrina Lo Brutto
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Correspondence to Sabrina Lo Brutto.

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Communicated by A. Schmidt-Rhaesa.

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Marrone, F., Deidun, A., Curatolo, T. et al. Species identification of the psammophilous tenebrionid beetles Phaleria acuminata Küster, 1852 and Phaleria bimaculata (Linnaeus, 1767) from central Mediterranean beaches: geometric morphometrics and molecular insights from species to population level. Zoomorphology 133, 71–82 (2014). https://doi.org/10.1007/s00435-013-0205-4

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