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Local Adaptation of Frankia to Different Discaria (Rhamnaceae) Host Species Growing in Patagonia

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Abstract

Frankia BCU110601 (Da) and Frankia BCU110345 (Dc) were isolated from root nodules of Discaria articulata and Discaria chacaye, respectively; Frankia BCU110501 (Dt) was previously isolated from Discaria trinervis. The strains were identical at the 16S sequence and after analysis of RFLP of 16S and 23S rDNA intergenic region. Diversity was revealed at the molecular level after fingerprint analysis by BOX–polymerase chain reaction. The strains were infective and effective on the original host plants. A cross-inoculation assay intra Discaria genus, including D. trinervis, D. articulata, and D. chacaye, with each of these isolated Frankia strains caused effective symbioses with a similar dry weight in each plant species regardless of the inoculated strain. Nevertheless, a differential degree of recognition was revealed: Homologous symbiotic pairs in the case of D. chacaye–Frankia BCU110345 (Dc), D. articulata–Frankia BCU110601 (Da), and D. trinervis–Frankia BCU110501 (Dt) had faster nodulation rates than heterologous pairs. The differences in nodulation rate would suggest the existence of a subspecific level of recognition within a certain cross-inoculation group, pointing to subspecific adaptation occurring in this actinorhizal symbiosis.

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Acknowledgments

We thank G. Vobis, M.I. Messuti, and M.M. Bunge for valuable comments. This work was supported by Universidad Nacional de Quilmes (grant No. PPUNQ 0340/03), CONICET (grant No. PIP-5812), and Universidad Nacional del Comahue. LGW and CV are researchers at CONICET (Argentina).

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Authors and Affiliations

  1. Centro Regional Universitario Bariloche, Universidad Nacional del Comahue, Quintral 1250, 8400 S.C., Bariloche, Argentina

    Eugenia E. Chaia

  2. Programa de Investigación en Interacciones Biológicas, Universidad Nacional de Quilmes, R. Sáenz Peña 352, B1876BXD, Bernal, Argentina

    Claudio Valverde & Luis G. Wall

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  1. Eugenia E. Chaia
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  2. Claudio Valverde
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  3. Luis G. Wall
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Correspondence to Luis G. Wall.

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Chaia, E.E., Valverde, C. & Wall, L.G. Local Adaptation of Frankia to Different Discaria (Rhamnaceae) Host Species Growing in Patagonia. Curr Microbiol 53, 523–528 (2006). https://doi.org/10.1007/s00284-006-0306-0

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  • DOI: https://doi.org/10.1007/s00284-006-0306-0

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