Abstract
Chemical composition, antimicrobial, antioxidant, and cytotoxic properties of Actinidia arguta essential oil (AEO) were evaluated. Gas chromatography–mass spectrometry analysis identified 56 chemical compounds, with the most abundant being Squalene (23.08%), γ-sitrostorol (8.10%), and β-Tocopherol (7.01%). Whereas the AEO had significant antimicrobial activity against Staphylococcus aureus and Saccharomyces cerevisiae, it showed mild efficacy against Bacillus subtilis and Microsporum canis. On the contrary, the Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, were not susceptible to the AEO pressure. On the other hand, the AEO exhibited strong antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), β-carotene, and hydroxyl radicals, with IC50 values of 117.60, 73.60, and 35.15 μg/mL, respectively. Additionally, compared to the PC-3 or HT-29 cell lines, the A549 cells were more susceptible to the AEO (IC50; 6.067 mg/mL). Besides, the confocal laser scanning microscopy imaging showed that 16 mg/mL of the AEO-induced apoptosis in the A549 cell lines. Our data indicated that the AEO might be useful in the food and pharmaceutical industry.
Graphical abstract
Preparation of Actinidia arguta essential oil (a) and schematic overview of the experiment (b)
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Abbreviations
- AEO:
-
A. arguta essential oil
- GC–MS:
-
Gas chromatography–mass spectrometry
- CLSM:
-
Confocal laser scanning microscopy
- DMEM:
-
Dulbecco’s Modified Eagle’s Medium
- FBS:
-
Fetal bovine serum
- RI:
-
Retention indices
- MIC:
-
Minimum inhibitory concentration
- MBC:
-
Minimum bactericidal concentration
- MFC:
-
Minimum fungicidal concentration
- DPPH:
-
1,1-diphenyl-2-picrylhydrazyl
- BHT:
-
Butylated hydroxytoluene
- MTT:
-
3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide
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We thank LetPub (https://www.letpub.com) for its linguistic assistance during the preparation of this manuscript.
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This work was supported by Natural Science Foundation Project of Liaoning province (Grant No. 2021-NLTS-14-02).
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HW and YY conceived and designed the experiments; HQ and ST performed the experiments; ZW and YY analyzed the experiment data; HW wrote the paper. All authors have read and agreed to the published version of the manuscript.
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Wang, H., Quan, H., Sun, T. et al. Chemical composition, antimicrobial, and antioxidant cytotoxic activities of essential oil from Actinidia arguta. Arch Microbiol 204, 239 (2022). https://doi.org/10.1007/s00203-022-02775-3
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DOI: https://doi.org/10.1007/s00203-022-02775-3