Comparative genomic hybridization (CGH) for intra- and interspecific comparisons in the female metaphase plates of Copeina guttata.
(a-d) Male- and female-derived genomic probes from C. guttata mapped against female chromosomes of C. guttata (e-h) Female-derived genomic probes from both Pyrrhulina brevis and C. guttata mapped against female chromosomes of C. guttata. (i-l) female-derived genomic probes from Lebiasina melanoguttata and C. guttata hybridized together against female chromosomes of C. guttata. First column (a, e, i) DAPI images (blue); second column (b, f, j): hybridization patterns using female gDNA of C. guttata (b), female-derived gDNA of P. brevis (f), and female gDNA of C. guttata (j); third column (c, g, k): hybridization patterns using male gDNA of C. guttata (C), female-derived gDNA of C. guttata (g), and female gDNA of Lebiasina melanoguttata (k). Fourth column (d, h, l): merged images of both genomic probes and DAPI staining. The common genomic regions are depicted in yellow.
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