Table 1.
Updated table of chromosomal data for Lebiasinidae fishes, adapted from [42].
Fig 1.
Amazon River basin area (in green), with the red dot indicating the collection site (Tefé, Amazonas state).
Fig 2.
Karyotypes of Copeina guttata male (a, b, c) and female (d, e, f) arranged from Giemsa-stained (a, d), C-banded chromosomes (b, e) and those after dual-color FISH with 18S (green) and 5S (red) rDNA probes (c, f). Chromosomes were counterstained with DAPI (blue). Scale bar = 5 μm.
Fig 3.
Comparative genomic hybridization (CGH) for intra- and interspecific comparisons in the female metaphase plates of Copeina guttata.
(a-d) Male- and female-derived genomic probes from C. guttata mapped against female chromosomes of C. guttata (e-h) Female-derived genomic probes from both Pyrrhulina brevis and C. guttata mapped against female chromosomes of C. guttata. (i-l) female-derived genomic probes from Lebiasina melanoguttata and C. guttata hybridized together against female chromosomes of C. guttata. First column (a, e, i) DAPI images (blue); second column (b, f, j): hybridization patterns using female gDNA of C. guttata (b), female-derived gDNA of P. brevis (f), and female gDNA of C. guttata (j); third column (c, g, k): hybridization patterns using male gDNA of C. guttata (C), female-derived gDNA of C. guttata (g), and female gDNA of Lebiasina melanoguttata (k). Fourth column (d, h, l): merged images of both genomic probes and DAPI staining. The common genomic regions are depicted in yellow.