Fig 1.
Halophila nipponica populations in the temperate waters of Korea and Japan.
Geographical locations for H. nipponica populations in Japanese waters were obtained from Uchimura et al. [15].
Table 1.
Collection information and GenBank accession numbers of Halophila nipponica specimens used for phylogenetic analysis from various geographical locations in the temperate coastal waters of northeast Asia.
Fig 2.
Phylogenetic tree of Halophila species inferred from maximum parsimony analysis using 655 base pairs of nrDNA including ITS1, 5.8S rDNA, and ITS2.
Bootstrap support values above 50% are shown on branches.
Table 2.
Similarities (%) and number of ITS sequence differences among five clades in section Halophila.
Fig 3.
Similarity of ITS sequences between Halophila nipponica and other Halophila species distributed in the Indo-Pacific and western Pacific regions.
Fig 4.
Relative divergence time estimates of five clades in the section Halophila (A), and the Halophila species (H. nipponica, H. okinawensis, and H. gaudichaudii) in Clade I and H. ovalis in Clade II (B).
Relative divergence time was estimated by ITS sequence diversity using the NETWORK 4.6 program. Values are mean ± SD.