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Fig 1.

Trollius ranunculoide flower with measured traits.

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Fig 2.

Location of the 12 studied populations of T. ranunculoides along an altitudinal gradient of southwest Gansu Province on the eastern Qinghai-Tibet Plateau.

The map was generated in ArcGIS 9.0 (ESRI, Environmental Systems Research Institute, Inc.).

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Fig 3.

Clinal variation in the floral traits and seed set of T. ranunculoides populations located along the altitudinal gradient of Gansu Province on the eastern Qinghai-Tibet Plateau.

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Fig 4.

Clinal variation of the visitation rate (visits plant-1 h-1) of all pollinators and main assemblages (flies and bees) across T. ranunculoides populations located along the altitudinal gradient.

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Table 1.

ANOVAs to test the variation of plant traits and visitation frequency of pollinators among populations of T. ranunculoides.

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Table 2.

Pearson’s correlation coefficients (r) for the floral traits measured in 12 populations of T. ranunculoides.

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Fig 5.

Selection differentials on sepal length T. ranunculoides along the altitudinal gradient.

Mean selection differential ± SE, each point represents a population. * indicates statistical significance.

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Fig 6.

Selection gradients on PC1 along the altitudinal gradient.

Mean selection gradient ± SE, each point indicates a population. PC1 represents the sepal length and width, which was from principal component analysis with varimax rotation. * indicates statistical significance.

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