Figure 1.
Pimelodella lateristriga. The asterisks indicate the cranial and caudal sections.
Figure 2.
(A) Light micrograph of the tegument showing an epithelial tissue (ET) supported by a connective tissue (CT) and a skeletal muscle tissue (MT). (B) Light micrograph of the stratified epithelial tissue composed by epidermal (EC) and club cells (CC). (C) Scanning electron micrograph showing the epithelial tissue with epidermal (EC) and club cells (CC), the connective tissue (CT) and the skeletal muscle tissue (MT). Scale bars: 25 µm (A); 15 µm (B) and 20 µm (C).
Figure 3.
Transmission electron micrographs of epidermis with emphasis in the epidermal cells.
The epidermis is composed by distinct cell types: club cell (CC), superficial epidermal (SEC), dense epidermal (DEC) and abundant epidermal (AEC). Scale bars: 10 µm (A) and 5 µm (B, C).
Figure 4.
Transmission electron micrographs of epidermis with emphasis in the club cells.
The epidermis is organized with conspicuous club cells (CC) and numerous small epidermal cell, as superficial epidermal (SEC), dense epidermal (DEC) and abundant epidermal (AEC) one. Note the connective tissue (CT) with melanophore (MP). Scale bars: 10 µm (A, B).
Figure 5.
Light (A), transmission (B) and scanning (C) micrographs of club cells.
The club cells (CC) possess a central nucleus (N) and are surrounded by epidermal cells (EC). In (C), presence of invaginations (arrows) of the club cell membrane. Connective tissue (CT). Scale bars: 24 µm (A); 10 µm (B) and 5 µm (C).
Figure 6.
Transmission electron micrographs of club cells.
(A) Club cell (CC) with central nucleus (N), membrane invaginations (arrows) and vacuoles (VA). Dense (DEC) and abundant (AEC) epidermal cells. (B) to (D) Perinuclear regions evidencing the presence of two nuclei in each club cell. The organelles are located next to the nuclei: mitochondria (mi), polirribossome (pr) and Golgi complex. Nucleoli (Nu). (E) Detail of the fibrillar cytoplasm (FC) with no vesicular secretion. Scale bars: 0.2 µm (E); 0.5 µm (A); 1 µm (C); 2 µm (B, D).
Table 1.
Club cells in the Pimelodella lateristriga skin (n.s. = not significant).
Figure 7.
(A) PAS method; (B) Bromophenol Blue method and (C) Mallory trichrome method. Epidermal cells (EC), club cells (CC), connective tissue (CT) and skeletal muscle tissue (MT). Scale bars: 15 µm.