Anti-inflammatory active ingredients of Hainan dischidia chinensis water extract and application

[0025] Embodiment 1: water extraction

[0026] 1 material

[0027] 1.1 Drugs and reagents Dischidiachinensis Champ was collected from the crater of Haikou City, Hainan Province, and was identified by Dai Haofu, a researcher at the Institute of Biology, Chinese Academy of Tropical Agricultural Sciences. .exBenth) whole plant. Aspirin enteric-coated tablets (Jiangsu Pingguang Pharmaceutical Co., Ltd., batch number: 140801); Evans blue (BS, 5g, Aladdin Reagent Shanghai Co., Ltd.); 0.9% sodium chloride injection (pharmaceutical grade, Hunan Jinjian Pharmaceutical Industry limited liability company); carboxymethylcellulose (AR, 500g / bottle, Sinopharm Chemical Reagent Factory); xylene (AR, 500mL / bottle, Tianjin Damao Pharmaceutical Chemical Reagent Factory); n-butanol, ethyl acetate and Petroleum ether was analytically pure. 1.2 Animals Kunming mice, clean grade, weighing 18-22 g, provided by Changsha Tianqin Biotechnology Co., Ltd., experimental animal use license number: SCXK (...

[0055] Take 5kg of dry Sapia praecox, crush it and pass it through a 40-mesh sieve, soak it in 30 times the amount of water overnight, then extract it with ultrasonic waves for 2 hours, the ultrasonic frequency is 40-50KHz, and the temperature is 70-80°C. After ultrasonic extraction for 1.5 hours, turn on at the same time Decoct in water for a total of 30 minutes, repeat the above-mentioned soaking, ultrasonication, and decoction 3 times, cool, filter, combine the filtrates, and concentrate under reduced pressure to an extract to obtain 229.5 g of water extract with a recovery rate of 4.59%. Take an appropriate amount of water extract and add 10 times of water to suspend to obtain a suspension, which is sequentially extracted with ethyl acetate, n-butanol, and petroleum ether. The volume ratios of ethyl acetate, n-butanol, petroleum ether and the suspension are 1:1, respectively. The extraction time was 10 min, respectively, and concentrated under reduced pressure to obtain eth...

[0057] Take 5kg of dry Sapia praecox, crush it and pass it through a 40-mesh sieve, soak it in 30 times the amount of water overnight, then extract it with ultrasonic waves for 2 hours, the ultrasonic frequency is 40-50KHz, and the temperature is 70-80°C. After ultrasonic extraction for 1.5 hours, turn on at the same time Decocting for a total of 30 minutes, repeating the above-mentioned soaking, ultrasonication, and decocting 3 times, cooling, filtering, combining the filtrates, and concentrating under reduced pressure to an extract form to obtain 229.5 g of water extract with a recovery rate of 4.59%. Take an appropriate amount of water extract and add 8 times of water to suspend to obtain a suspension, and then extract it with ethyl acetate, n-butanol, and petroleum ether. The volume ratios of ethyl acetate, n-butanol, petroleum ether and the suspension are 1:2, respectively. The extraction time was 6 minutes respectively, and concentrated under reduced pressure to obtain et...