2. Metarhizium anisopliae
• formerly known as Entomophthora anisopliae,
is a widely distributed soil-inhabiting fungus.
• Elie Metchnikoff ( Russian biologist) who first
experimentally tests it as microbial agent against
insects in 1879
• categorized as a green muscardine fungus due
to the green color of the sporulating colonies.
3. • has been reported to infect approximately
200 species of insects and arthropods
• is not infectious or toxic to mammals
• Beauveria bassiana and Metarhizium
anisopliae, for control of adult malaria
mosquitoes
M. anisopliae
5. CHARACTERISTICS
• the optimal temperature was 25 degrees C
• uses a combination of enzymes and
mechanical force to penetrate the host
cuticle
• has robust conidia that are easy to formulate
and store
M. anisopliae
6. HABITAT
• Metarhizium species are commonly
thought of as soil saprophytes and are
most frequently found in disturbed habitats
like agricultural fields as compared to forest
ecosystems (Meyling and Eilenberg 2007).
• these fungi form associations with plant
roots in the rhizosphere (Hu and St. Leger
2002)
M. anisopliae
7. LIFE CYCLE
The life cycle of species within this
genus will be restricted to their activity
as arthropod pathogens.
The development has 3 stages:
M. anisopliae
8.
9. Mode of action
• The spores of this fungus when come in
contact with the cuticle (skin) of
susceptible insects, they germinate and
grow directly through the cuticle
• proliferates throughout the insect’s body
and draining the insect of nutrients,
eventually killing it.
10. TOLERANCE
• They found that all of these isolates shared
similar conidial morphology but some
showed the ability to germinate at colder
temperatures (i.e. 5 C
M. anisopliae
11. Commercial Availability
• Faria and Wraight (2007) determined that
there were 47 different commercially-
available Metarhizium-based products
available around the world
• different products were listed as
either Metarhizium anisopliae or Metarhiziu
m anisopliae var. acridum, which Green
Muscle is based on.
M. anisopliae
12. USES
• used as a biological insecticide to control
a number of pests as Grasshoppers,
Termites,Thrips, catterpillers
..aphids/etc.
• use in the control of malaria-transmitting
mosquitos